Advancing antimicrobial susceptibility testing in body fluid-associated infections: a focus on direct testing for timely and targeted therapeutic interventions
Background: timely Antimicrobial Susceptibility Testing (AST) is critical in managing infections, particularly with the rise of multidrug-resistant organisms. This study evaluates the efficacy of Direct AST (D-AST) on body fluids in reducing turnaround time compared to Routine AST (R-AST). Materi...
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PAGEPress Publications
2025-08-01
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| Series: | Microbiologia Medica |
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| Online Access: | https://www.pagepressjournals.org/mm/article/view/13602 |
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| author | Nisha Goyal Vikas Saini Nimisha Jain Bandi Shravana Shukla Das Narendra Pal Singh Meenakshi Goswami |
| author_facet | Nisha Goyal Vikas Saini Nimisha Jain Bandi Shravana Shukla Das Narendra Pal Singh Meenakshi Goswami |
| author_sort | Nisha Goyal |
| collection | DOAJ |
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Background: timely Antimicrobial Susceptibility Testing (AST) is critical in managing infections, particularly with the rise of multidrug-resistant organisms. This study evaluates the efficacy of Direct AST (D-AST) on body fluids in reducing turnaround time compared to Routine AST (R-AST).
Materials and Methods: over six months, 43 monobacterial body fluid samples were analyzed using both D-AST and R-AST at a tertiary care hospital. D-AST was performed on the same day of sample receipt, while R-AST followed Clinical and Laboratory Standards Institute (CLSI) guidelines. Concordance between the methods was statistically assessed.
Results: D-AST showed high concordance with R-AST for most antibiotics (p>0.05), with a significant reduction in turnaround time (D-AST: 24-36 hours; R-AST: 48-72 hours). For Gram-negative organisms, discrepancies were noted for amikacin sensitivity in Escherichia coli (D-AST: 76.19%; R-AST: 90.4%) and ciprofloxacin in Klebsiella pneumoniae (D-AST: 80%; R-AST: 100%). Gram-positive organisms showed 100% concordance for key antibiotics like linezolid and teicoplanin.
Conclusions: D-AST is a reliable, rapid alternative to R-AST, enabling earlier therapeutic decisions and reducing empirical antibiotic use. It shows promise in improving outcomes and combating antimicrobial resistance, particularly in resource-limited settings. Further standardization is needed for broader application.
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| format | Article |
| id | doaj-art-12a227fb414c47caafe32269a150a1f7 |
| institution | Kabale University |
| issn | 2280-6423 |
| language | English |
| publishDate | 2025-08-01 |
| publisher | PAGEPress Publications |
| record_format | Article |
| series | Microbiologia Medica |
| spelling | doaj-art-12a227fb414c47caafe32269a150a1f72025-08-20T03:35:32ZengPAGEPress PublicationsMicrobiologia Medica2280-64232025-08-0140110.4081/mm.2025.13602Advancing antimicrobial susceptibility testing in body fluid-associated infections: a focus on direct testing for timely and targeted therapeutic interventionsNisha Goyal0Vikas Saini1Nimisha Jain2Bandi Shravana3Shukla Das4Narendra Pal Singh5Meenakshi Goswami6Department of Microbiology, University College of Medical Sciences and Guru Tag Bahadur Hospital, DelhiDepartment of Microbiology, Satyawadi Raja Harish Chand Hospital, DelhiUniversity College of Medical Sciences and Guru Tag Bahadur Hospital, DelhiDepartment of Microbiology, University College of Medical Sciences and Guru Tag Bahadur Hospital, DelhiDepartment of Microbiology, University College of Medical Sciences and Guru Tag Bahadur Hospital, DelhiDepartment of Microbiology, Super Speciality Paediatric Hospital & Post Graduate Teaching Institute, NoidaDepartment of Microbiology, University College of Medical Sciences and Guru Tag Bahadur Hospital, Delhi Background: timely Antimicrobial Susceptibility Testing (AST) is critical in managing infections, particularly with the rise of multidrug-resistant organisms. This study evaluates the efficacy of Direct AST (D-AST) on body fluids in reducing turnaround time compared to Routine AST (R-AST). Materials and Methods: over six months, 43 monobacterial body fluid samples were analyzed using both D-AST and R-AST at a tertiary care hospital. D-AST was performed on the same day of sample receipt, while R-AST followed Clinical and Laboratory Standards Institute (CLSI) guidelines. Concordance between the methods was statistically assessed. Results: D-AST showed high concordance with R-AST for most antibiotics (p>0.05), with a significant reduction in turnaround time (D-AST: 24-36 hours; R-AST: 48-72 hours). For Gram-negative organisms, discrepancies were noted for amikacin sensitivity in Escherichia coli (D-AST: 76.19%; R-AST: 90.4%) and ciprofloxacin in Klebsiella pneumoniae (D-AST: 80%; R-AST: 100%). Gram-positive organisms showed 100% concordance for key antibiotics like linezolid and teicoplanin. Conclusions: D-AST is a reliable, rapid alternative to R-AST, enabling earlier therapeutic decisions and reducing empirical antibiotic use. It shows promise in improving outcomes and combating antimicrobial resistance, particularly in resource-limited settings. Further standardization is needed for broader application. https://www.pagepressjournals.org/mm/article/view/13602Direct Antimicrobial Susceptibility Testing (D-AST)body fluid infectionsAntimicrobial Resistance (AMR)rapid diagnostic methodsempirical therapy reduction |
| spellingShingle | Nisha Goyal Vikas Saini Nimisha Jain Bandi Shravana Shukla Das Narendra Pal Singh Meenakshi Goswami Advancing antimicrobial susceptibility testing in body fluid-associated infections: a focus on direct testing for timely and targeted therapeutic interventions Microbiologia Medica Direct Antimicrobial Susceptibility Testing (D-AST) body fluid infections Antimicrobial Resistance (AMR) rapid diagnostic methods empirical therapy reduction |
| title | Advancing antimicrobial susceptibility testing in body fluid-associated infections: a focus on direct testing for timely and targeted therapeutic interventions |
| title_full | Advancing antimicrobial susceptibility testing in body fluid-associated infections: a focus on direct testing for timely and targeted therapeutic interventions |
| title_fullStr | Advancing antimicrobial susceptibility testing in body fluid-associated infections: a focus on direct testing for timely and targeted therapeutic interventions |
| title_full_unstemmed | Advancing antimicrobial susceptibility testing in body fluid-associated infections: a focus on direct testing for timely and targeted therapeutic interventions |
| title_short | Advancing antimicrobial susceptibility testing in body fluid-associated infections: a focus on direct testing for timely and targeted therapeutic interventions |
| title_sort | advancing antimicrobial susceptibility testing in body fluid associated infections a focus on direct testing for timely and targeted therapeutic interventions |
| topic | Direct Antimicrobial Susceptibility Testing (D-AST) body fluid infections Antimicrobial Resistance (AMR) rapid diagnostic methods empirical therapy reduction |
| url | https://www.pagepressjournals.org/mm/article/view/13602 |
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