Molecular features of head and neck squamous cell carcinoma
Relevance. To identify new markers of early diagnosis and prognosis of head and neck squamous cell carcinoma (HNSCC) it is necessary to study the molecular features of this disease.Purpose. The aim of the study was to analyze blood serum protein spectrum in patients with HNSCC and in healthy volunte...
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Siberian State Medical University (Tomsk)
2018-09-01
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| Series: | Бюллетень сибирской медицины |
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| Online Access: | https://bulletin.ssmu.ru/jour/article/view/1284 |
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| author | G. V. Kakurina E. S. Kolegova О. V. Cheremisina Е. L. Choinzonov |
| author_facet | G. V. Kakurina E. S. Kolegova О. V. Cheremisina Е. L. Choinzonov |
| author_sort | G. V. Kakurina |
| collection | DOAJ |
| description | Relevance. To identify new markers of early diagnosis and prognosis of head and neck squamous cell carcinoma (HNSCC) it is necessary to study the molecular features of this disease.Purpose. The aim of the study was to analyze blood serum protein spectrum in patients with HNSCC and in healthy volunteers using the methods of mass spectrometry and to evaluate the selected serum protein markers as candidates for early detection of HNSCC.Materials and Methods: The blood serum of HNSCC patients before therapy with metastases, without metastases and healthy volunteers was studied by proteomic methods. Validation of the results of proteomic analysis was carried out by ELISA in serum of 52 patients with HNSCC (T1-4N0-3M0), 10 patients with chronic hyperplastic laryngitis, dysplasia DII-DIII and 10 healthy volunteers. The statistical analysis was carried out using Statistica 6.0. Software package.Results. Blood serum proteome of HNSCC patients with metastases, without metastases and healthy volunteers are different and contain proteins of different classes. Adenylyl cyclase-associated protein 1 (CAP1) and protein phosphatase 1B (PPM1B) were selected to validate the obtained results. It was shown that the serum level of CAP1 and PPM1B differed in control and dysplasia groups and dysplasia and cancer groups (p ≤ 0,05). In patients with HNSCC (T1N0M0) the serum CAP1 and PPM1B levels were higher than in patients with dysplasia and healthy individuals (p ≤ 0,05). It was noted the positive correlation of the CAP1 level in the serum with the presence of metastases and the PPM1B level.Conclusion. Candidates for serum markers of HNSCC prognosis were identified. The difference in serum levels of CAP1 and PPM1B depending on the prevalence of primary tumors and the difference in serum level of CAP1 depending on the presence of regional metastases was shown. Determination of CAP1 level in the serum can be useful for early diagnosis and prognosis of HNSCC. |
| format | Article |
| id | doaj-art-129b2a8f154b44128b6958498f969bf7 |
| institution | DOAJ |
| issn | 1682-0363 1819-3684 |
| language | English |
| publishDate | 2018-09-01 |
| publisher | Siberian State Medical University (Tomsk) |
| record_format | Article |
| series | Бюллетень сибирской медицины |
| spelling | doaj-art-129b2a8f154b44128b6958498f969bf72025-08-20T03:02:05ZengSiberian State Medical University (Tomsk)Бюллетень сибирской медицины1682-03631819-36842018-09-01173616910.20538/1682-0363-2018-3-61-69808Molecular features of head and neck squamous cell carcinomaG. V. Kakurina0E. S. Kolegova1О. V. Cheremisina2Е. L. Choinzonov3Cancer Research Institute, Tomsk National Research Medical Center (TNRМС) of Russian Academy of Science (RAS)Cancer Research Institute, Tomsk National Research Medical Center (TNRМС) of Russian Academy of Science (RAS); Siberian State Medical University (SSMU)Cancer Research Institute, Tomsk National Research Medical Center (TNRМС) of Russian Academy of Science (RAS)Cancer Research Institute, Tomsk National Research Medical Center (TNRМС) of Russian Academy of Science (RAS); Siberian State Medical University (SSMU)Relevance. To identify new markers of early diagnosis and prognosis of head and neck squamous cell carcinoma (HNSCC) it is necessary to study the molecular features of this disease.Purpose. The aim of the study was to analyze blood serum protein spectrum in patients with HNSCC and in healthy volunteers using the methods of mass spectrometry and to evaluate the selected serum protein markers as candidates for early detection of HNSCC.Materials and Methods: The blood serum of HNSCC patients before therapy with metastases, without metastases and healthy volunteers was studied by proteomic methods. Validation of the results of proteomic analysis was carried out by ELISA in serum of 52 patients with HNSCC (T1-4N0-3M0), 10 patients with chronic hyperplastic laryngitis, dysplasia DII-DIII and 10 healthy volunteers. The statistical analysis was carried out using Statistica 6.0. Software package.Results. Blood serum proteome of HNSCC patients with metastases, without metastases and healthy volunteers are different and contain proteins of different classes. Adenylyl cyclase-associated protein 1 (CAP1) and protein phosphatase 1B (PPM1B) were selected to validate the obtained results. It was shown that the serum level of CAP1 and PPM1B differed in control and dysplasia groups and dysplasia and cancer groups (p ≤ 0,05). In patients with HNSCC (T1N0M0) the serum CAP1 and PPM1B levels were higher than in patients with dysplasia and healthy individuals (p ≤ 0,05). It was noted the positive correlation of the CAP1 level in the serum with the presence of metastases and the PPM1B level.Conclusion. Candidates for serum markers of HNSCC prognosis were identified. The difference in serum levels of CAP1 and PPM1B depending on the prevalence of primary tumors and the difference in serum level of CAP1 depending on the presence of regional metastases was shown. Determination of CAP1 level in the serum can be useful for early diagnosis and prognosis of HNSCC.https://bulletin.ssmu.ru/jour/article/view/1284chronic hyperplastic laryngitis and pharyngitisepithelial dysplasiasquamous cell carcinoma of head and neckadenylyl cyclase-associated protein 1рrotein phosphatase 1b |
| spellingShingle | G. V. Kakurina E. S. Kolegova О. V. Cheremisina Е. L. Choinzonov Molecular features of head and neck squamous cell carcinoma Бюллетень сибирской медицины chronic hyperplastic laryngitis and pharyngitis epithelial dysplasia squamous cell carcinoma of head and neck adenylyl cyclase-associated protein 1 рrotein phosphatase 1b |
| title | Molecular features of head and neck squamous cell carcinoma |
| title_full | Molecular features of head and neck squamous cell carcinoma |
| title_fullStr | Molecular features of head and neck squamous cell carcinoma |
| title_full_unstemmed | Molecular features of head and neck squamous cell carcinoma |
| title_short | Molecular features of head and neck squamous cell carcinoma |
| title_sort | molecular features of head and neck squamous cell carcinoma |
| topic | chronic hyperplastic laryngitis and pharyngitis epithelial dysplasia squamous cell carcinoma of head and neck adenylyl cyclase-associated protein 1 рrotein phosphatase 1b |
| url | https://bulletin.ssmu.ru/jour/article/view/1284 |
| work_keys_str_mv | AT gvkakurina molecularfeaturesofheadandnecksquamouscellcarcinoma AT eskolegova molecularfeaturesofheadandnecksquamouscellcarcinoma AT ovcheremisina molecularfeaturesofheadandnecksquamouscellcarcinoma AT elchoinzonov molecularfeaturesofheadandnecksquamouscellcarcinoma |