Carbon fluxes rewiring in engineered E. coli via reverse tricarboxylic acid cycle pathway under chemolithotrophic condition

Carbon fluxes rewiring in engineered E. coli via reverse tricarboxylic acid cycle pathway under chemolithotrophic condition

Abstract Background A transgenic strain of Escherichia coli has been engineered to directly assimilate gaseous CO2 into its biomass through hydrogen-powered anaerobic respiration. This was achieved by expressing key components of the reverse tricarboxylic acid (rTCA) cycle, including genes encoding...

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Main Authors: Jian-Hau Peng, Shou-Chen Lo, Yu-Ning Yu, Ya-Tang Yang, Yu-Chieh Chen, An-I Tsai, Dong-Yan Wu, Chu-Han Huang, Tien-Tsai Su, Chieh-Chen Huang, En-Pei Isabel Chiang
Format: Article
Language:English
Published: BMC 2025-02-01
Series:Journal of Biological Engineering
Subjects:
Escherichia coli
Reverse tricarboxylic acid cycle
Carbon flux rewiring
CO2 incorporation
Protein and nucleotide biosynthesis
Online Access:https://doi.org/10.1186/s13036-025-00489-w
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