Efficacy of <i>Stenocereus queretaroensis</i>-Derived Silver Nanoparticles Against Methicillin-Resistant <i>Staphylococcus aureus</i> Biofilms: Synthesis and Antibiofilm Activity
Methicillin-resistant <i>Staphylococcus aureus</i> (MRSA) is responsible for many infections, primarily due to its ability to form biofilms. Nanotechnology has recently been proposed as an alternative for controlling MRSA. In the present work, we evaluated the antimicrobial and antibiofi...
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2025-05-01
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| author | Angélica Sofía González-Garibay Iván Moisés Sánchez-Hernández Ariadna Abigail Villarreal-Amézquita Omar Ricardo Torres-González Eduardo Padilla-Camberos |
| author_facet | Angélica Sofía González-Garibay Iván Moisés Sánchez-Hernández Ariadna Abigail Villarreal-Amézquita Omar Ricardo Torres-González Eduardo Padilla-Camberos |
| author_sort | Angélica Sofía González-Garibay |
| collection | DOAJ |
| description | Methicillin-resistant <i>Staphylococcus aureus</i> (MRSA) is responsible for many infections, primarily due to its ability to form biofilms. Nanotechnology has recently been proposed as an alternative for controlling MRSA. In the present work, we evaluated the antimicrobial and antibiofilm activities of silver nanoparticles synthesized with <i>Stenocereus queretaroensis</i> peel extract (SAgNPs). The biosynthesis process was optimized using a response surface design. The results showed antimicrobial activity against MRSA bacteria, with a minimum inhibitory concentration and minimum bactericidal concentration of 0.15 and 0.31 µg/mL, respectively. SAgNPs inhibited biofilm formation in multi-well plates and Congo red agar. Molecular docking analysis revealed that the presence of quercetin, one of the chemical components of <i>S. queretaroensis</i> peel, forms hydrogen bonds with six interacting amino acids. This suggests that quercetin presents a stable binding to this site, which in turn suggests that the mechanism of action of SAgNPs is related to their binding to PBP2a. Therefore, these findings suggest a promising, environmentally friendly approach to combating antibiotic-resistant infections, potentially reducing the reliance on traditional antibiotics. |
| format | Article |
| id | doaj-art-12837608f3da47c0834d01f372fe345c |
| institution | Kabale University |
| issn | 2304-6740 |
| language | English |
| publishDate | 2025-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Inorganics |
| spelling | doaj-art-12837608f3da47c0834d01f372fe345c2025-08-20T03:27:29ZengMDPI AGInorganics2304-67402025-05-0113617810.3390/inorganics13060178Efficacy of <i>Stenocereus queretaroensis</i>-Derived Silver Nanoparticles Against Methicillin-Resistant <i>Staphylococcus aureus</i> Biofilms: Synthesis and Antibiofilm ActivityAngélica Sofía González-Garibay0Iván Moisés Sánchez-Hernández1Ariadna Abigail Villarreal-Amézquita2Omar Ricardo Torres-González3Eduardo Padilla-Camberos4Unit of Medical and Pharmaceutical Biotechnology, Center for Research and Assistance in Technology and Design of the State of Jalisco, A.C. (CIATEJ), Guadalajara 44270, Jalisco, MexicoUnit of Medical and Pharmaceutical Biotechnology, Center for Research and Assistance in Technology and Design of the State of Jalisco, A.C. (CIATEJ), Guadalajara 44270, Jalisco, MexicoUnit of Medical and Pharmaceutical Biotechnology, Center for Research and Assistance in Technology and Design of the State of Jalisco, A.C. (CIATEJ), Guadalajara 44270, Jalisco, MexicoUnit of Medical and Pharmaceutical Biotechnology, Center for Research and Assistance in Technology and Design of the State of Jalisco, A.C. (CIATEJ), Guadalajara 44270, Jalisco, MexicoUnit of Medical and Pharmaceutical Biotechnology, Center for Research and Assistance in Technology and Design of the State of Jalisco, A.C. (CIATEJ), Guadalajara 44270, Jalisco, MexicoMethicillin-resistant <i>Staphylococcus aureus</i> (MRSA) is responsible for many infections, primarily due to its ability to form biofilms. Nanotechnology has recently been proposed as an alternative for controlling MRSA. In the present work, we evaluated the antimicrobial and antibiofilm activities of silver nanoparticles synthesized with <i>Stenocereus queretaroensis</i> peel extract (SAgNPs). The biosynthesis process was optimized using a response surface design. The results showed antimicrobial activity against MRSA bacteria, with a minimum inhibitory concentration and minimum bactericidal concentration of 0.15 and 0.31 µg/mL, respectively. SAgNPs inhibited biofilm formation in multi-well plates and Congo red agar. Molecular docking analysis revealed that the presence of quercetin, one of the chemical components of <i>S. queretaroensis</i> peel, forms hydrogen bonds with six interacting amino acids. This suggests that quercetin presents a stable binding to this site, which in turn suggests that the mechanism of action of SAgNPs is related to their binding to PBP2a. Therefore, these findings suggest a promising, environmentally friendly approach to combating antibiotic-resistant infections, potentially reducing the reliance on traditional antibiotics.https://www.mdpi.com/2304-6740/13/6/178antibiofilmmetallic nanoparticlesantibiotic resistance |
| spellingShingle | Angélica Sofía González-Garibay Iván Moisés Sánchez-Hernández Ariadna Abigail Villarreal-Amézquita Omar Ricardo Torres-González Eduardo Padilla-Camberos Efficacy of <i>Stenocereus queretaroensis</i>-Derived Silver Nanoparticles Against Methicillin-Resistant <i>Staphylococcus aureus</i> Biofilms: Synthesis and Antibiofilm Activity Inorganics antibiofilm metallic nanoparticles antibiotic resistance |
| title | Efficacy of <i>Stenocereus queretaroensis</i>-Derived Silver Nanoparticles Against Methicillin-Resistant <i>Staphylococcus aureus</i> Biofilms: Synthesis and Antibiofilm Activity |
| title_full | Efficacy of <i>Stenocereus queretaroensis</i>-Derived Silver Nanoparticles Against Methicillin-Resistant <i>Staphylococcus aureus</i> Biofilms: Synthesis and Antibiofilm Activity |
| title_fullStr | Efficacy of <i>Stenocereus queretaroensis</i>-Derived Silver Nanoparticles Against Methicillin-Resistant <i>Staphylococcus aureus</i> Biofilms: Synthesis and Antibiofilm Activity |
| title_full_unstemmed | Efficacy of <i>Stenocereus queretaroensis</i>-Derived Silver Nanoparticles Against Methicillin-Resistant <i>Staphylococcus aureus</i> Biofilms: Synthesis and Antibiofilm Activity |
| title_short | Efficacy of <i>Stenocereus queretaroensis</i>-Derived Silver Nanoparticles Against Methicillin-Resistant <i>Staphylococcus aureus</i> Biofilms: Synthesis and Antibiofilm Activity |
| title_sort | efficacy of i stenocereus queretaroensis i derived silver nanoparticles against methicillin resistant i staphylococcus aureus i biofilms synthesis and antibiofilm activity |
| topic | antibiofilm metallic nanoparticles antibiotic resistance |
| url | https://www.mdpi.com/2304-6740/13/6/178 |
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