Comprehensive landscape of small non‐coding RNAs in large and small extracellular vesicles from human serum and plasma
Abstract Small non‐coding RNAs (ncRNAs) are functional molecules contained within extracellular vesicles (EVs) that modulate various physiological and pathological processes. This study provides a comprehensive expression profile of seven types of small ncRNAs in serum‐ and plasma‐derived EVs under...
Saved in:
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley-VCH
2025-03-01
|
| Series: | Interdisciplinary Medicine |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/INMD.20240073 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849341184389939200 |
|---|---|
| author | Gui‐Yan Xie Yi‐Han Deng Chun‐Jie Liu An‐Yuan Guo Qian Lei |
| author_facet | Gui‐Yan Xie Yi‐Han Deng Chun‐Jie Liu An‐Yuan Guo Qian Lei |
| author_sort | Gui‐Yan Xie |
| collection | DOAJ |
| description | Abstract Small non‐coding RNAs (ncRNAs) are functional molecules contained within extracellular vesicles (EVs) that modulate various physiological and pathological processes. This study provides a comprehensive expression profile of seven types of small ncRNAs in serum‐ and plasma‐derived EVs under various conditions. Both large EVs (lEVs) and small EVs (sEVs) contain high proportions of miRNAs (∼28.2% in lEVs and ∼20.8% in sEVs) and ribosomal RNAs (∼24.0% in lEVs and ∼19.1% in sEVs). lEVs are enriched with more transfer RNA (∼38.8%) than sEVs, whereas sEVs have a greater abundance of Y RNA (∼22.5%). Notably, Y RNA is more abundant in sEVs obtained from aged samples (age ≥60 years), a pattern not observed in lEVs. Small ncRNAs are more diverse in serum‐derived EVs than plasma‐derived EVs. There is also a high degree of overlap (>50%) in the top 100 small ncRNAs identified in lEVs and sEVs. The miRNAs hsa‐miR‐16‐5p, hsa‐let‐7a‐5p, hsa‐miR‐142‐3p, and hsa‐miR‐103‐3p are consistently among the top 10 highly expressed small ncRNAs in plasma‐ and serum‐derived lEVs as well as in peripheral blood mononuclear cells. Serum‐derived sEVs in glioblastoma, breast cancer, prostate cancer, and gastric cancer contain specific, highly expressed miRNAs, snoRNAs, small nuclear RNAs, and piRNAs. These results elucidate the patterns of small ncRNA cargoes within lEVs and sEVs derived from serum and plasma under various physiological and pathological conditions and offer valuable insights for future diagnostic and therapeutic applications. |
| format | Article |
| id | doaj-art-128335a577314233beb5fb7e66332a4a |
| institution | Kabale University |
| issn | 2832-6245 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Wiley-VCH |
| record_format | Article |
| series | Interdisciplinary Medicine |
| spelling | doaj-art-128335a577314233beb5fb7e66332a4a2025-08-20T03:43:41ZengWiley-VCHInterdisciplinary Medicine2832-62452025-03-0132n/an/a10.1002/INMD.20240073Comprehensive landscape of small non‐coding RNAs in large and small extracellular vesicles from human serum and plasmaGui‐Yan Xie0Yi‐Han Deng1Chun‐Jie Liu2An‐Yuan Guo3Qian Lei4Department of Thoracic Surgery West China Biomedical Big Data Center West China Hospital Med‐X Center for Informatics Sichuan University Chengdu ChinaHubei Bioinformatics & Molecular Imaging Key Laboratory Huazhong University of Science and Technology Wuhan ChinaDepartment of Thoracic Surgery West China Biomedical Big Data Center West China Hospital Med‐X Center for Informatics Sichuan University Chengdu ChinaDepartment of Thoracic Surgery West China Biomedical Big Data Center West China Hospital Med‐X Center for Informatics Sichuan University Chengdu ChinaDepartment of Thoracic Surgery West China Biomedical Big Data Center West China Hospital Med‐X Center for Informatics Sichuan University Chengdu ChinaAbstract Small non‐coding RNAs (ncRNAs) are functional molecules contained within extracellular vesicles (EVs) that modulate various physiological and pathological processes. This study provides a comprehensive expression profile of seven types of small ncRNAs in serum‐ and plasma‐derived EVs under various conditions. Both large EVs (lEVs) and small EVs (sEVs) contain high proportions of miRNAs (∼28.2% in lEVs and ∼20.8% in sEVs) and ribosomal RNAs (∼24.0% in lEVs and ∼19.1% in sEVs). lEVs are enriched with more transfer RNA (∼38.8%) than sEVs, whereas sEVs have a greater abundance of Y RNA (∼22.5%). Notably, Y RNA is more abundant in sEVs obtained from aged samples (age ≥60 years), a pattern not observed in lEVs. Small ncRNAs are more diverse in serum‐derived EVs than plasma‐derived EVs. There is also a high degree of overlap (>50%) in the top 100 small ncRNAs identified in lEVs and sEVs. The miRNAs hsa‐miR‐16‐5p, hsa‐let‐7a‐5p, hsa‐miR‐142‐3p, and hsa‐miR‐103‐3p are consistently among the top 10 highly expressed small ncRNAs in plasma‐ and serum‐derived lEVs as well as in peripheral blood mononuclear cells. Serum‐derived sEVs in glioblastoma, breast cancer, prostate cancer, and gastric cancer contain specific, highly expressed miRNAs, snoRNAs, small nuclear RNAs, and piRNAs. These results elucidate the patterns of small ncRNA cargoes within lEVs and sEVs derived from serum and plasma under various physiological and pathological conditions and offer valuable insights for future diagnostic and therapeutic applications.https://doi.org/10.1002/INMD.20240073expression profileslEVsplasmaserumsEVssmall ncRNAs |
| spellingShingle | Gui‐Yan Xie Yi‐Han Deng Chun‐Jie Liu An‐Yuan Guo Qian Lei Comprehensive landscape of small non‐coding RNAs in large and small extracellular vesicles from human serum and plasma Interdisciplinary Medicine expression profiles lEVs plasma serum sEVs small ncRNAs |
| title | Comprehensive landscape of small non‐coding RNAs in large and small extracellular vesicles from human serum and plasma |
| title_full | Comprehensive landscape of small non‐coding RNAs in large and small extracellular vesicles from human serum and plasma |
| title_fullStr | Comprehensive landscape of small non‐coding RNAs in large and small extracellular vesicles from human serum and plasma |
| title_full_unstemmed | Comprehensive landscape of small non‐coding RNAs in large and small extracellular vesicles from human serum and plasma |
| title_short | Comprehensive landscape of small non‐coding RNAs in large and small extracellular vesicles from human serum and plasma |
| title_sort | comprehensive landscape of small non coding rnas in large and small extracellular vesicles from human serum and plasma |
| topic | expression profiles lEVs plasma serum sEVs small ncRNAs |
| url | https://doi.org/10.1002/INMD.20240073 |
| work_keys_str_mv | AT guiyanxie comprehensivelandscapeofsmallnoncodingrnasinlargeandsmallextracellularvesiclesfromhumanserumandplasma AT yihandeng comprehensivelandscapeofsmallnoncodingrnasinlargeandsmallextracellularvesiclesfromhumanserumandplasma AT chunjieliu comprehensivelandscapeofsmallnoncodingrnasinlargeandsmallextracellularvesiclesfromhumanserumandplasma AT anyuanguo comprehensivelandscapeofsmallnoncodingrnasinlargeandsmallextracellularvesiclesfromhumanserumandplasma AT qianlei comprehensivelandscapeofsmallnoncodingrnasinlargeandsmallextracellularvesiclesfromhumanserumandplasma |