Evaluation of Serodiagnostic Assays for Mycobacterium bovis Infection in Elk, White-Tailed Deer, and Reindeer in the United States
In 2011, the United States Department of Agriculture conducted a project in which elk (Cervus elaphus spp.), white-tailed deer (WTD) (Odocoileus virginianus), and reindeer (Rangifer tarandus) were evaluated by the single cervical tuberculin test (SCT), comparative cervical tuberculin test (CCT), and...
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| Format: | Article |
| Language: | English |
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Wiley
2012-01-01
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| Series: | Veterinary Medicine International |
| Online Access: | http://dx.doi.org/10.1155/2012/563293 |
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| author | Jeffrey T. Nelson Kathleen A. Orloski Audra L. Lloyd Mark Camacho Mark A. Schoenbaum Suelee Robbe-Austerman Bruce V. Thomsen S. Mark Hall |
| author_facet | Jeffrey T. Nelson Kathleen A. Orloski Audra L. Lloyd Mark Camacho Mark A. Schoenbaum Suelee Robbe-Austerman Bruce V. Thomsen S. Mark Hall |
| author_sort | Jeffrey T. Nelson |
| collection | DOAJ |
| description | In 2011, the United States Department of Agriculture conducted a project in which elk (Cervus elaphus spp.), white-tailed deer (WTD) (Odocoileus virginianus), and reindeer (Rangifer tarandus) were evaluated by the single cervical tuberculin test (SCT), comparative cervical tuberculin test (CCT), and serologic tests. The rapid antibody detection tests evaluated were the CervidTB Stat-Pak (Stat-Pak), and the Dual Path Platform VetTB (DPP). Blood was collected from presumably uninfected animals prior to tuberculin injection for the SCT. A total of 1,783 animals were enrolled in the project. Of these, 1,752 (98.3%) were classified as presumably uninfected, based on originating from a captive cervid herd with no history of exposure to TB. Stat-Pak specificity estimates were 92.4% in reindeer, 96.7% in WTD, and 98.3% in elk and were not significantly different from SCT specificity estimates. Using the DPP in series on Stat-Pak antibody-positive samples improved specificity in the three species. Thirty one animals were classified as confirmed infected, based on necropsy and laboratory results, and 27/31 were antibody positive on Stat-Pak for an estimated sensitivity of 87.1%. The study findings indicate that rapid serologic tests used in series are comparable to the SCT and CCT and may have a greater ability to detect TB-infected cervids. |
| format | Article |
| id | doaj-art-1271d59992ac436bbc8d4839e270a9c6 |
| institution | Kabale University |
| issn | 2090-8113 2042-0048 |
| language | English |
| publishDate | 2012-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Veterinary Medicine International |
| spelling | doaj-art-1271d59992ac436bbc8d4839e270a9c62025-08-20T03:54:20ZengWileyVeterinary Medicine International2090-81132042-00482012-01-01201210.1155/2012/563293563293Evaluation of Serodiagnostic Assays for Mycobacterium bovis Infection in Elk, White-Tailed Deer, and Reindeer in the United StatesJeffrey T. Nelson0Kathleen A. Orloski1Audra L. Lloyd2Mark Camacho3Mark A. Schoenbaum4Suelee Robbe-Austerman5Bruce V. Thomsen6S. Mark Hall7National Veterinary Services Laboratories, Veterinary Services (VS), Animal and Plant Health Inspection Service (APHIS), United States Department of Agriculture (USDA), Ames, IA 50010, USAVS, APHIS, USDA, Fort Collins, CO 80526-8117, USANational Veterinary Services Laboratories, Veterinary Services (VS), Animal and Plant Health Inspection Service (APHIS), United States Department of Agriculture (USDA), Ames, IA 50010, USAVS, APHIS, USDA, Raleigh, NC 27606, USAVS, APHIS, USDA, Fort Collins, CO 80526-8117, USANational Veterinary Services Laboratories, Veterinary Services (VS), Animal and Plant Health Inspection Service (APHIS), United States Department of Agriculture (USDA), Ames, IA 50010, USANational Veterinary Services Laboratories, Veterinary Services (VS), Animal and Plant Health Inspection Service (APHIS), United States Department of Agriculture (USDA), Ames, IA 50010, USANational Veterinary Services Laboratories, Veterinary Services (VS), Animal and Plant Health Inspection Service (APHIS), United States Department of Agriculture (USDA), Ames, IA 50010, USAIn 2011, the United States Department of Agriculture conducted a project in which elk (Cervus elaphus spp.), white-tailed deer (WTD) (Odocoileus virginianus), and reindeer (Rangifer tarandus) were evaluated by the single cervical tuberculin test (SCT), comparative cervical tuberculin test (CCT), and serologic tests. The rapid antibody detection tests evaluated were the CervidTB Stat-Pak (Stat-Pak), and the Dual Path Platform VetTB (DPP). Blood was collected from presumably uninfected animals prior to tuberculin injection for the SCT. A total of 1,783 animals were enrolled in the project. Of these, 1,752 (98.3%) were classified as presumably uninfected, based on originating from a captive cervid herd with no history of exposure to TB. Stat-Pak specificity estimates were 92.4% in reindeer, 96.7% in WTD, and 98.3% in elk and were not significantly different from SCT specificity estimates. Using the DPP in series on Stat-Pak antibody-positive samples improved specificity in the three species. Thirty one animals were classified as confirmed infected, based on necropsy and laboratory results, and 27/31 were antibody positive on Stat-Pak for an estimated sensitivity of 87.1%. The study findings indicate that rapid serologic tests used in series are comparable to the SCT and CCT and may have a greater ability to detect TB-infected cervids.http://dx.doi.org/10.1155/2012/563293 |
| spellingShingle | Jeffrey T. Nelson Kathleen A. Orloski Audra L. Lloyd Mark Camacho Mark A. Schoenbaum Suelee Robbe-Austerman Bruce V. Thomsen S. Mark Hall Evaluation of Serodiagnostic Assays for Mycobacterium bovis Infection in Elk, White-Tailed Deer, and Reindeer in the United States Veterinary Medicine International |
| title | Evaluation of Serodiagnostic Assays for Mycobacterium bovis Infection in Elk, White-Tailed Deer, and Reindeer in the United States |
| title_full | Evaluation of Serodiagnostic Assays for Mycobacterium bovis Infection in Elk, White-Tailed Deer, and Reindeer in the United States |
| title_fullStr | Evaluation of Serodiagnostic Assays for Mycobacterium bovis Infection in Elk, White-Tailed Deer, and Reindeer in the United States |
| title_full_unstemmed | Evaluation of Serodiagnostic Assays for Mycobacterium bovis Infection in Elk, White-Tailed Deer, and Reindeer in the United States |
| title_short | Evaluation of Serodiagnostic Assays for Mycobacterium bovis Infection in Elk, White-Tailed Deer, and Reindeer in the United States |
| title_sort | evaluation of serodiagnostic assays for mycobacterium bovis infection in elk white tailed deer and reindeer in the united states |
| url | http://dx.doi.org/10.1155/2012/563293 |
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