Detection of OqxAB Efflux Pumps, a Multidrug-Resistant Agent in Bacterial Infection in Patients Referring to Teaching Hospitals in Ahvaz, Southwest of Iran

Antibiotic resistance mechanisms in Enterobacteriaceae are causative agents of global health problems. Bacterial infections due to multidrug resistance (MDR) may be mediated by the overexpression of efflux pumps. In this study, we investigated the prevalence of oqxA and oqxB genes as two encoding ag...

Full description

Saved in:
Bibliographic Details
Main Authors: Mojtaba Moosavian, Mahtab Khoshkholgh Sima, Nazanin Ahmad Khosravi, Effat Abbasi Montazeri
Format: Article
Language:English
Published: Wiley 2021-01-01
Series:International Journal of Microbiology
Online Access:http://dx.doi.org/10.1155/2021/2145176
Tags: Add Tag
No Tags, Be the first to tag this record!
_version_ 1832547060569604096
author Mojtaba Moosavian
Mahtab Khoshkholgh Sima
Nazanin Ahmad Khosravi
Effat Abbasi Montazeri
author_facet Mojtaba Moosavian
Mahtab Khoshkholgh Sima
Nazanin Ahmad Khosravi
Effat Abbasi Montazeri
author_sort Mojtaba Moosavian
collection DOAJ
description Antibiotic resistance mechanisms in Enterobacteriaceae are causative agents of global health problems. Bacterial infections due to multidrug resistance (MDR) may be mediated by the overexpression of efflux pumps. In this study, we investigated the prevalence of oqxA and oqxB genes as two encoding agents of efflux pumps and the determination of antibiotic resistance rate in clinical isolates of Enterobacteriaceae. In this study, 100 Enterobacteriaceae isolates collected from different clinical specimens of infectious patients, such as wounds, urine, blood, discharge, and abscesses except stool, were examined. Identification of the isolates was performed using standard biochemical tests such as TSI, citrate, urea, lysine, SIM, MR-VP, and gas production. The antimicrobial susceptibility test was carried out by the Kirby–Bauer disk diffusion method according to CLSI guidelines, and finally, the oqxA and oqxB genes were detected by the PCR method. Among 100 Enterobacteriaceae isolates, Escherichia coli and Enterobacter gergoviae were the most common isolates with 71% and 20%, respectively. Also, the lowest isolates belonged to Enterobacter cloacae (3%) and Klebsiella pneumoniae (1%). Out of 100 Enterobacteriaceae isolates, 37 isolates (37%) were positive for at least one of oqxA or oqxB genes, while both of these genes were detected among 12% of them. oqxAB genes were detected in 8 cases of 20 (40%) Enterobacter gergoviae and 4 cases of 71 (5.7%) E. coli isolates. The antimicrobial susceptibility test showed that all isolates (100%) were susceptible to imipenem, while the maximum resistance to piperacillin, ceftriaxone, and cefotaxime were 69%, 55%, and 55%, respectively. Also, the results of this study showed that antibiotic resistance in Enterobacteriaceae isolates caused by oqxAB genes is increasing among patients in Iran. Therefore, identification of resistant isolates and antibiotic monitoring programs are essential to prevent the spread of MDR isolates.
format Article
id doaj-art-126af391f235430cb20a41b61e4f4748
institution Kabale University
issn 1687-9198
language English
publishDate 2021-01-01
publisher Wiley
record_format Article
series International Journal of Microbiology
spelling doaj-art-126af391f235430cb20a41b61e4f47482025-02-03T06:46:01ZengWileyInternational Journal of Microbiology1687-91982021-01-01202110.1155/2021/2145176Detection of OqxAB Efflux Pumps, a Multidrug-Resistant Agent in Bacterial Infection in Patients Referring to Teaching Hospitals in Ahvaz, Southwest of IranMojtaba Moosavian0Mahtab Khoshkholgh Sima1Nazanin Ahmad Khosravi2Effat Abbasi Montazeri3Infectious and Tropical Diseases Research CenterDepartment of MicrobiologyDepartment of MicrobiologyDepartment of MicrobiologyAntibiotic resistance mechanisms in Enterobacteriaceae are causative agents of global health problems. Bacterial infections due to multidrug resistance (MDR) may be mediated by the overexpression of efflux pumps. In this study, we investigated the prevalence of oqxA and oqxB genes as two encoding agents of efflux pumps and the determination of antibiotic resistance rate in clinical isolates of Enterobacteriaceae. In this study, 100 Enterobacteriaceae isolates collected from different clinical specimens of infectious patients, such as wounds, urine, blood, discharge, and abscesses except stool, were examined. Identification of the isolates was performed using standard biochemical tests such as TSI, citrate, urea, lysine, SIM, MR-VP, and gas production. The antimicrobial susceptibility test was carried out by the Kirby–Bauer disk diffusion method according to CLSI guidelines, and finally, the oqxA and oqxB genes were detected by the PCR method. Among 100 Enterobacteriaceae isolates, Escherichia coli and Enterobacter gergoviae were the most common isolates with 71% and 20%, respectively. Also, the lowest isolates belonged to Enterobacter cloacae (3%) and Klebsiella pneumoniae (1%). Out of 100 Enterobacteriaceae isolates, 37 isolates (37%) were positive for at least one of oqxA or oqxB genes, while both of these genes were detected among 12% of them. oqxAB genes were detected in 8 cases of 20 (40%) Enterobacter gergoviae and 4 cases of 71 (5.7%) E. coli isolates. The antimicrobial susceptibility test showed that all isolates (100%) were susceptible to imipenem, while the maximum resistance to piperacillin, ceftriaxone, and cefotaxime were 69%, 55%, and 55%, respectively. Also, the results of this study showed that antibiotic resistance in Enterobacteriaceae isolates caused by oqxAB genes is increasing among patients in Iran. Therefore, identification of resistant isolates and antibiotic monitoring programs are essential to prevent the spread of MDR isolates.http://dx.doi.org/10.1155/2021/2145176
spellingShingle Mojtaba Moosavian
Mahtab Khoshkholgh Sima
Nazanin Ahmad Khosravi
Effat Abbasi Montazeri
Detection of OqxAB Efflux Pumps, a Multidrug-Resistant Agent in Bacterial Infection in Patients Referring to Teaching Hospitals in Ahvaz, Southwest of Iran
International Journal of Microbiology
title Detection of OqxAB Efflux Pumps, a Multidrug-Resistant Agent in Bacterial Infection in Patients Referring to Teaching Hospitals in Ahvaz, Southwest of Iran
title_full Detection of OqxAB Efflux Pumps, a Multidrug-Resistant Agent in Bacterial Infection in Patients Referring to Teaching Hospitals in Ahvaz, Southwest of Iran
title_fullStr Detection of OqxAB Efflux Pumps, a Multidrug-Resistant Agent in Bacterial Infection in Patients Referring to Teaching Hospitals in Ahvaz, Southwest of Iran
title_full_unstemmed Detection of OqxAB Efflux Pumps, a Multidrug-Resistant Agent in Bacterial Infection in Patients Referring to Teaching Hospitals in Ahvaz, Southwest of Iran
title_short Detection of OqxAB Efflux Pumps, a Multidrug-Resistant Agent in Bacterial Infection in Patients Referring to Teaching Hospitals in Ahvaz, Southwest of Iran
title_sort detection of oqxab efflux pumps a multidrug resistant agent in bacterial infection in patients referring to teaching hospitals in ahvaz southwest of iran
url http://dx.doi.org/10.1155/2021/2145176
work_keys_str_mv AT mojtabamoosavian detectionofoqxabeffluxpumpsamultidrugresistantagentinbacterialinfectioninpatientsreferringtoteachinghospitalsinahvazsouthwestofiran
AT mahtabkhoshkholghsima detectionofoqxabeffluxpumpsamultidrugresistantagentinbacterialinfectioninpatientsreferringtoteachinghospitalsinahvazsouthwestofiran
AT nazaninahmadkhosravi detectionofoqxabeffluxpumpsamultidrugresistantagentinbacterialinfectioninpatientsreferringtoteachinghospitalsinahvazsouthwestofiran
AT effatabbasimontazeri detectionofoqxabeffluxpumpsamultidrugresistantagentinbacterialinfectioninpatientsreferringtoteachinghospitalsinahvazsouthwestofiran