Heterogeneous Chromosomal Aberrations in Intraductal Breast Lesions Adjacent to Invasive Carcinoma
There is evidence that breast cancer is a heterogeneous disease phenotypically as well as molecular biologically. So far, heterogeneity on the molecular biological level has not been investigated in potential precursor lesions, such as ductal hyperplasia (DH) and ductal carcinoma in situ (DCIS). In...
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| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2000-01-01
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| Series: | Analytical Cellular Pathology |
| Online Access: | http://dx.doi.org/10.1155/2000/930246 |
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| _version_ | 1832555905426653184 |
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| author | Michaela Aubele Margaret Cummings Axel Walch Horst Zitzelsberger Jörg Nährig Heinz Höfler Martin Werner |
| author_facet | Michaela Aubele Margaret Cummings Axel Walch Horst Zitzelsberger Jörg Nährig Heinz Höfler Martin Werner |
| author_sort | Michaela Aubele |
| collection | DOAJ |
| description | There is evidence that breast cancer is a heterogeneous disease phenotypically as well as molecular biologically. So far, heterogeneity on the molecular biological level has not been investigated in potential precursor lesions, such as ductal hyperplasia (DH) and ductal carcinoma in situ (DCIS). In this study we applied comparative genomic hybridization (CGH) to formalin‐fixed, paraffin‐embedded breast tissue with DH and DCIS, adjacent to invasive ductal carcinoma (IDC), to screen these potential precursor lesions for whole genomic chromosomal imbalances. Laser‐microdissection was used to select pure cell populations from the sections. Isolated DNA was amplified by degenerate oligonucleotide primed PCR (DOP‐PCR) and further processed for CGH analysis. |
| format | Article |
| id | doaj-art-1261106808e74083809da99885fde117 |
| institution | Kabale University |
| issn | 0921-8912 1878-3651 |
| language | English |
| publishDate | 2000-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Analytical Cellular Pathology |
| spelling | doaj-art-1261106808e74083809da99885fde1172025-02-03T05:46:52ZengWileyAnalytical Cellular Pathology0921-89121878-36512000-01-01201172410.1155/2000/930246Heterogeneous Chromosomal Aberrations in Intraductal Breast Lesions Adjacent to Invasive CarcinomaMichaela Aubele0Margaret Cummings1Axel Walch2Horst Zitzelsberger3Jörg Nährig4Heinz Höfler5Martin Werner6GSF‐National Research Center for Environment and Health, Institute of Pathology, Neuherberg, GermanyDepartment of Pathology, University of Queensland, Medical School, Queensland, AustraliaGSF‐National Research Center for Environment and Health, Institute of Pathology, Neuherberg, GermanyGSF‐National Research Center for Environment and Health, Institute of Radiobiology, Neuherberg, GermanyTechnische Universität München, Institute of Pathology, Munich, GermanyGSF‐National Research Center for Environment and Health, Institute of Pathology, Neuherberg, GermanyTechnische Universität München, Institute of Pathology, Munich, GermanyThere is evidence that breast cancer is a heterogeneous disease phenotypically as well as molecular biologically. So far, heterogeneity on the molecular biological level has not been investigated in potential precursor lesions, such as ductal hyperplasia (DH) and ductal carcinoma in situ (DCIS). In this study we applied comparative genomic hybridization (CGH) to formalin‐fixed, paraffin‐embedded breast tissue with DH and DCIS, adjacent to invasive ductal carcinoma (IDC), to screen these potential precursor lesions for whole genomic chromosomal imbalances. Laser‐microdissection was used to select pure cell populations from the sections. Isolated DNA was amplified by degenerate oligonucleotide primed PCR (DOP‐PCR) and further processed for CGH analysis.http://dx.doi.org/10.1155/2000/930246 |
| spellingShingle | Michaela Aubele Margaret Cummings Axel Walch Horst Zitzelsberger Jörg Nährig Heinz Höfler Martin Werner Heterogeneous Chromosomal Aberrations in Intraductal Breast Lesions Adjacent to Invasive Carcinoma Analytical Cellular Pathology |
| title | Heterogeneous Chromosomal Aberrations in Intraductal Breast Lesions Adjacent to Invasive Carcinoma |
| title_full | Heterogeneous Chromosomal Aberrations in Intraductal Breast Lesions Adjacent to Invasive Carcinoma |
| title_fullStr | Heterogeneous Chromosomal Aberrations in Intraductal Breast Lesions Adjacent to Invasive Carcinoma |
| title_full_unstemmed | Heterogeneous Chromosomal Aberrations in Intraductal Breast Lesions Adjacent to Invasive Carcinoma |
| title_short | Heterogeneous Chromosomal Aberrations in Intraductal Breast Lesions Adjacent to Invasive Carcinoma |
| title_sort | heterogeneous chromosomal aberrations in intraductal breast lesions adjacent to invasive carcinoma |
| url | http://dx.doi.org/10.1155/2000/930246 |
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