Simulated gastrointestinal digestion of milk caseins containing A1 β-casein does not lead to increased production of inflammatory proteins in intestinal epithelial cells in vitro when compared to milk caseins containing A2 β-casein.
Gastrointestinal digestion of bovine milk protein β-casein releases a biologically active peptide β-casomorphin (BCM)-7, which has been associated with increased incidence of non-communicable diseases and negative effects on digestive comfort. In this study, casein isolates containing both β-casein...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-02-01
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| Series: | Journal of Functional Foods |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1756464625000118 |
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| author | Alan Aitchison Teagan S. Edwards Andrew S. Day |
| author_facet | Alan Aitchison Teagan S. Edwards Andrew S. Day |
| author_sort | Alan Aitchison |
| collection | DOAJ |
| description | Gastrointestinal digestion of bovine milk protein β-casein releases a biologically active peptide β-casomorphin (BCM)-7, which has been associated with increased incidence of non-communicable diseases and negative effects on digestive comfort. In this study, casein isolates containing both β-casein isoforms were enzymatically digested using a previously described method. The digestion products were incubated with intestinal epithelial cells with or without the presence of an inflammatory cytokine, interleukin (IL)-1β, and the effects on inflammatory markers studied. BCM-7 was released from digestion of both β-casein isoforms although higher concentrations were released from A1 β-casein. No difference was found in the effects of the digestion products of each β-casein on gene expression of inflammatory markers interleukin (IL)-8, carcinoembryonic antigen-related cell adhesion molecule (CEACAM)6 or intestinal alkaline phosphatase (IAP). Together, the results of this study do not provide insight for the reported health benefits of milk containing A2 β-casein over milk containing the A1 isoform. |
| format | Article |
| id | doaj-art-1256c07fa4f746d28f00725e04e7670c |
| institution | Kabale University |
| issn | 1756-4646 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Functional Foods |
| spelling | doaj-art-1256c07fa4f746d28f00725e04e7670c2025-02-09T04:59:54ZengElsevierJournal of Functional Foods1756-46462025-02-01125106669Simulated gastrointestinal digestion of milk caseins containing A1 β-casein does not lead to increased production of inflammatory proteins in intestinal epithelial cells in vitro when compared to milk caseins containing A2 β-casein.Alan Aitchison0Teagan S. Edwards1Andrew S. Day2Corresponding author.; Department of Paediatrics, University of Otago Christchurch, Christchurch, New ZealandDepartment of Paediatrics, University of Otago Christchurch, Christchurch, New ZealandDepartment of Paediatrics, University of Otago Christchurch, Christchurch, New ZealandGastrointestinal digestion of bovine milk protein β-casein releases a biologically active peptide β-casomorphin (BCM)-7, which has been associated with increased incidence of non-communicable diseases and negative effects on digestive comfort. In this study, casein isolates containing both β-casein isoforms were enzymatically digested using a previously described method. The digestion products were incubated with intestinal epithelial cells with or without the presence of an inflammatory cytokine, interleukin (IL)-1β, and the effects on inflammatory markers studied. BCM-7 was released from digestion of both β-casein isoforms although higher concentrations were released from A1 β-casein. No difference was found in the effects of the digestion products of each β-casein on gene expression of inflammatory markers interleukin (IL)-8, carcinoembryonic antigen-related cell adhesion molecule (CEACAM)6 or intestinal alkaline phosphatase (IAP). Together, the results of this study do not provide insight for the reported health benefits of milk containing A2 β-casein over milk containing the A1 isoform.http://www.sciencedirect.com/science/article/pii/S1756464625000118β-Caseinβ-Casomorphin-7 (BCM-7)Bovine milkInflammation |
| spellingShingle | Alan Aitchison Teagan S. Edwards Andrew S. Day Simulated gastrointestinal digestion of milk caseins containing A1 β-casein does not lead to increased production of inflammatory proteins in intestinal epithelial cells in vitro when compared to milk caseins containing A2 β-casein. Journal of Functional Foods β-Casein β-Casomorphin-7 (BCM-7) Bovine milk Inflammation |
| title | Simulated gastrointestinal digestion of milk caseins containing A1 β-casein does not lead to increased production of inflammatory proteins in intestinal epithelial cells in vitro when compared to milk caseins containing A2 β-casein. |
| title_full | Simulated gastrointestinal digestion of milk caseins containing A1 β-casein does not lead to increased production of inflammatory proteins in intestinal epithelial cells in vitro when compared to milk caseins containing A2 β-casein. |
| title_fullStr | Simulated gastrointestinal digestion of milk caseins containing A1 β-casein does not lead to increased production of inflammatory proteins in intestinal epithelial cells in vitro when compared to milk caseins containing A2 β-casein. |
| title_full_unstemmed | Simulated gastrointestinal digestion of milk caseins containing A1 β-casein does not lead to increased production of inflammatory proteins in intestinal epithelial cells in vitro when compared to milk caseins containing A2 β-casein. |
| title_short | Simulated gastrointestinal digestion of milk caseins containing A1 β-casein does not lead to increased production of inflammatory proteins in intestinal epithelial cells in vitro when compared to milk caseins containing A2 β-casein. |
| title_sort | simulated gastrointestinal digestion of milk caseins containing a1 β casein does not lead to increased production of inflammatory proteins in intestinal epithelial cells in vitro when compared to milk caseins containing a2 β casein |
| topic | β-Casein β-Casomorphin-7 (BCM-7) Bovine milk Inflammation |
| url | http://www.sciencedirect.com/science/article/pii/S1756464625000118 |
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