Aspergillus Niger 129B promotes plant growth by inducing the interaction between SAUR32 and PP2C72
IntroductionPlant growth-promoting fungi (PGPF) play a fundamental role in plant development, such as nutrient acquisition and root growth. However, the growth promotion mechanisms regulated by Aspergillus niger are poorly characterized.MethodsWe examined the growth-promoting effects of Aspergillus...
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Frontiers Media S.A.
2025-08-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2025.1637235/full |
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| author | Juan He Li Yuan Gao Yong Ping Luo Min Chai Hua Feng Wang Lu Hong Yang Kang Zhao Yushanjiang Maimaiti Yuan Jun Nie Wei Chen |
| author_facet | Juan He Li Yuan Gao Yong Ping Luo Min Chai Hua Feng Wang Lu Hong Yang Kang Zhao Yushanjiang Maimaiti Yuan Jun Nie Wei Chen |
| author_sort | Juan He |
| collection | DOAJ |
| description | IntroductionPlant growth-promoting fungi (PGPF) play a fundamental role in plant development, such as nutrient acquisition and root growth. However, the growth promotion mechanisms regulated by Aspergillus niger are poorly characterized.MethodsWe examined the growth-promoting effects of Aspergillus niger 129B on tomato plant root, stem, and leaf development through a combination of phenotype analyses and plant biomass measurement. Subsequently, molecular and genetic experiments were conducted to reveal the mechanism promoting root, stem, and leaf development.ResultsIt demonstrated that 129B significantly promoted the growth of tomato plants. Plant transcriptome and metabolome analysis revealed that this effect was associated with the plant hormone signaling pathway, particularly the expression of SAUR32 and PP2C72 genes. In addition, 129B could promote the development of root, stem, and leaf tissues by downregulating the expression of SAUR32 and PP2C72 genes. Importantly, we found that the promotion of tissue development may be attributed to the interaction between SAUR32 and PP2C72; the expression of SAUR32 proteins, which act as inhibitors of PP2C72 phosphatases, triggered root H+ efflux.DiscussionOur findings concluded that 129B-induced plant promotion is dependent on the interaction between SAUR32 and PP2C72, providing novel insights into beneficial plant–microbiome interactions. |
| format | Article |
| id | doaj-art-124e3c7dc5b549ecaf8ec3f7443de10f |
| institution | Kabale University |
| issn | 1664-302X |
| language | English |
| publishDate | 2025-08-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Microbiology |
| spelling | doaj-art-124e3c7dc5b549ecaf8ec3f7443de10f2025-08-26T04:13:10ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2025-08-011610.3389/fmicb.2025.16372351637235Aspergillus Niger 129B promotes plant growth by inducing the interaction between SAUR32 and PP2C72Juan He0Li Yuan Gao1Yong Ping Luo2Min Chai3Hua Feng Wang4Lu Hong Yang5Kang Zhao6Yushanjiang Maimaiti7Yuan Jun Nie8Wei Chen9School of Life Science, Shanxi Normal University, Taiyuan, ChinaSchool of Life Science, Shanxi Normal University, Taiyuan, ChinaSchool of Life Science, Shanxi Normal University, Taiyuan, ChinaSchool of Life Science, Shanxi Normal University, Taiyuan, ChinaSchool of Life Science, Shanxi Normal University, Taiyuan, ChinaSchool of Life Science, Shanxi Normal University, Taiyuan, ChinaSchool of Life Science, Shanxi Normal University, Taiyuan, ChinaXinjiang Institute of Plant Protection, Ürümqi, ChinaCollege of Agricultural Economics and Management, Shanxi Agricultural University, Taiyuan, ChinaSchool of Life Science, Shanxi Normal University, Taiyuan, ChinaIntroductionPlant growth-promoting fungi (PGPF) play a fundamental role in plant development, such as nutrient acquisition and root growth. However, the growth promotion mechanisms regulated by Aspergillus niger are poorly characterized.MethodsWe examined the growth-promoting effects of Aspergillus niger 129B on tomato plant root, stem, and leaf development through a combination of phenotype analyses and plant biomass measurement. Subsequently, molecular and genetic experiments were conducted to reveal the mechanism promoting root, stem, and leaf development.ResultsIt demonstrated that 129B significantly promoted the growth of tomato plants. Plant transcriptome and metabolome analysis revealed that this effect was associated with the plant hormone signaling pathway, particularly the expression of SAUR32 and PP2C72 genes. In addition, 129B could promote the development of root, stem, and leaf tissues by downregulating the expression of SAUR32 and PP2C72 genes. Importantly, we found that the promotion of tissue development may be attributed to the interaction between SAUR32 and PP2C72; the expression of SAUR32 proteins, which act as inhibitors of PP2C72 phosphatases, triggered root H+ efflux.DiscussionOur findings concluded that 129B-induced plant promotion is dependent on the interaction between SAUR32 and PP2C72, providing novel insights into beneficial plant–microbiome interactions.https://www.frontiersin.org/articles/10.3389/fmicb.2025.1637235/fullPGPFA. nigerplant hormone signaling pathwayplant growthtomato |
| spellingShingle | Juan He Li Yuan Gao Yong Ping Luo Min Chai Hua Feng Wang Lu Hong Yang Kang Zhao Yushanjiang Maimaiti Yuan Jun Nie Wei Chen Aspergillus Niger 129B promotes plant growth by inducing the interaction between SAUR32 and PP2C72 Frontiers in Microbiology PGPF A. niger plant hormone signaling pathway plant growth tomato |
| title | Aspergillus Niger 129B promotes plant growth by inducing the interaction between SAUR32 and PP2C72 |
| title_full | Aspergillus Niger 129B promotes plant growth by inducing the interaction between SAUR32 and PP2C72 |
| title_fullStr | Aspergillus Niger 129B promotes plant growth by inducing the interaction between SAUR32 and PP2C72 |
| title_full_unstemmed | Aspergillus Niger 129B promotes plant growth by inducing the interaction between SAUR32 and PP2C72 |
| title_short | Aspergillus Niger 129B promotes plant growth by inducing the interaction between SAUR32 and PP2C72 |
| title_sort | aspergillus niger 129b promotes plant growth by inducing the interaction between saur32 and pp2c72 |
| topic | PGPF A. niger plant hormone signaling pathway plant growth tomato |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2025.1637235/full |
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