Human miscarriage and infection in Tunisia: Role of Mycoplasma hominis and high Waddlia seroprevalence
Introduction: Miscarriage is one of the most common adverse pregnancy outcomes. The aim of this study was to investigate the relationship between miscarriage in humans and infections caused by zoonotic bacteria and genital pathogens. Methodology: Cervicovaginal swabs and placenta samples from 13...
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The Journal of Infection in Developing Countries
2019-05-01
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| Series: | Journal of Infection in Developing Countries |
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| Online Access: | https://jidc.org/index.php/journal/article/view/9829 |
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| author | Mariem Smaoui Kebbi Carole Hanen Sellami Salma Kammoun Khaled Choura Leila Maazoun Houssem Mestiri Sebastien Aeby Doulira Louati Mohamed Derbel Kais Chaabene Adnene Hammami Gilbert Greub Abir Znazen |
| author_facet | Mariem Smaoui Kebbi Carole Hanen Sellami Salma Kammoun Khaled Choura Leila Maazoun Houssem Mestiri Sebastien Aeby Doulira Louati Mohamed Derbel Kais Chaabene Adnene Hammami Gilbert Greub Abir Znazen |
| author_sort | Mariem Smaoui |
| collection | DOAJ |
| description |
Introduction: Miscarriage is one of the most common adverse pregnancy outcomes. The aim of this study was to investigate the relationship between miscarriage in humans and infections caused by zoonotic bacteria and genital pathogens.
Methodology: Cervicovaginal swabs and placenta samples from 132 women with miscarriage (patient group: PG), and cervicovaginal swabs from 54 women with normal pregnancy (control group:CG), were subjected to bacteriological culture and real time PCRs detecting Coxiella burnetii, Brucella spp, Mycoplasma hominis, Mycoplasma genitalium, Ureaplasma urealyticum, Chlamydia trachomatis, Waddlia chondrophila and Parachlamydia acanthamoebeae DNA. Serology of C. burnetii, C. trachomatis and W. chondrophila was also performed.
Results: Placenta samples were positive for E. coli, S. agalactiae, U. urealyticum, M. hominis and C. trachomatis in 4.7%, 3.1%, 3.1%, 0.7% and 0.7% of cases, respectively. For cervicovaginal swabs, M. hominis was more frequently detected among PG than CG with a significant statistical difference (p = 0.02). C. trachomatis was detected in 3.3% and 5.5% among PG and CG, respectively. U. urealyticum DNA was detected with high percentages in the two groups. Samples from both groups showed negatives results for C. burnetii, Waddlia, and Brucella qPCRs. A high rate of W. chondrophila seroprevalence (42%) was noted with significant difference among women with early miscarriage.
Conclusions: C. trachomatis, S. agalactiae and M. hominis may play a role in miscarriage. However, the full characterization of the vaginal flora using other technologies such as NGS-based metagenomics is needed to clarify their role in miscarriage. Finally, further investigations should be performed to explain high W. chondrophila seroprevalence.
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| format | Article |
| id | doaj-art-12366c1873384dbc8ab4b5e356a8471e |
| institution | OA Journals |
| issn | 1972-2680 |
| language | English |
| publishDate | 2019-05-01 |
| publisher | The Journal of Infection in Developing Countries |
| record_format | Article |
| series | Journal of Infection in Developing Countries |
| spelling | doaj-art-12366c1873384dbc8ab4b5e356a8471e2025-08-20T02:27:09ZengThe Journal of Infection in Developing CountriesJournal of Infection in Developing Countries1972-26802019-05-01130510.3855/jidc.9829Human miscarriage and infection in Tunisia: Role of Mycoplasma hominis and high Waddlia seroprevalenceMariem Smaoui0Kebbi Carole1Hanen Sellami2Salma Kammoun3Khaled Choura4Leila Maazoun5Houssem Mestiri6Sebastien Aeby7Doulira Louati8Mohamed Derbel9Kais Chaabene10Adnene Hammami11Gilbert Greub12Abir Znazen13Laboratory of Microbiology, Research Laboratory “MPH”, Habib Bourguiba University Hopsital of Sfax, Sfax University, Sfax, TunisiaCenter for Research on Intracellular Bacteria (CRIB), Institute of Microbiology, University of Lausanne and University Hospital of Lausanne, Lausanne, SwitzerlandLaboratory of Microbiology, Research Laboratory “MPH”, Habib Bourguiba University Hopsital of Sfax, Sfax University ,Sfax, TunisiaMaternity Department, Hedi Chaker University hospital of Sfax, Sfax, TunisiaMaternity Department, Hedi Chaker University hospital of Sfax, Sfax, TunisiaFamily Planning Office, Sfax, TunisiaMaternity Department, Mahres Regional Hospital, Sfax, TunisiaCenter for Research on Intracellular Bacteria (CRIB), Institute of Microbiology, University of Lausanne and University Hospital of Lausanne, Lausanne, SwitzerlandMaternity Department, Hedi Chaker University hospital of Sfax, Sfax, TunisiaMaternity Department, Hedi Chaker University hospital of Sfax, Sfax, TunisiaMaternity Department, Hedi Chaker University hospital of Sfax, Sfax, TunisiaLaboratory of Microbiology, Research Laboratory “MPH”, Habib Bourguiba University Hopsital of Sfax, Sfax University, Tunisia AND Laboratory of treatment and valorization of wastewater (CERTE). Borj-Cedria technopark, Tunisia.Center for Research on Intracellular Bacteria (CRIB), Institute of Microbiology, University of Lausanne and University Hospital of Lausanne, Lausanne , SwitzerlandLaboratory of Microbiology, Research Laboratory “MPH”, Habib Bourguiba University Hopsital of Sfax, Sfax University ,Sfax, Tunisia Introduction: Miscarriage is one of the most common adverse pregnancy outcomes. The aim of this study was to investigate the relationship between miscarriage in humans and infections caused by zoonotic bacteria and genital pathogens. Methodology: Cervicovaginal swabs and placenta samples from 132 women with miscarriage (patient group: PG), and cervicovaginal swabs from 54 women with normal pregnancy (control group:CG), were subjected to bacteriological culture and real time PCRs detecting Coxiella burnetii, Brucella spp, Mycoplasma hominis, Mycoplasma genitalium, Ureaplasma urealyticum, Chlamydia trachomatis, Waddlia chondrophila and Parachlamydia acanthamoebeae DNA. Serology of C. burnetii, C. trachomatis and W. chondrophila was also performed. Results: Placenta samples were positive for E. coli, S. agalactiae, U. urealyticum, M. hominis and C. trachomatis in 4.7%, 3.1%, 3.1%, 0.7% and 0.7% of cases, respectively. For cervicovaginal swabs, M. hominis was more frequently detected among PG than CG with a significant statistical difference (p = 0.02). C. trachomatis was detected in 3.3% and 5.5% among PG and CG, respectively. U. urealyticum DNA was detected with high percentages in the two groups. Samples from both groups showed negatives results for C. burnetii, Waddlia, and Brucella qPCRs. A high rate of W. chondrophila seroprevalence (42%) was noted with significant difference among women with early miscarriage. Conclusions: C. trachomatis, S. agalactiae and M. hominis may play a role in miscarriage. However, the full characterization of the vaginal flora using other technologies such as NGS-based metagenomics is needed to clarify their role in miscarriage. Finally, further investigations should be performed to explain high W. chondrophila seroprevalence. https://jidc.org/index.php/journal/article/view/9829miscarriagegenital infectionzoonosisgenital mycoplasmasWaddlia |
| spellingShingle | Mariem Smaoui Kebbi Carole Hanen Sellami Salma Kammoun Khaled Choura Leila Maazoun Houssem Mestiri Sebastien Aeby Doulira Louati Mohamed Derbel Kais Chaabene Adnene Hammami Gilbert Greub Abir Znazen Human miscarriage and infection in Tunisia: Role of Mycoplasma hominis and high Waddlia seroprevalence Journal of Infection in Developing Countries miscarriage genital infection zoonosis genital mycoplasmas Waddlia |
| title | Human miscarriage and infection in Tunisia: Role of Mycoplasma hominis and high Waddlia seroprevalence |
| title_full | Human miscarriage and infection in Tunisia: Role of Mycoplasma hominis and high Waddlia seroprevalence |
| title_fullStr | Human miscarriage and infection in Tunisia: Role of Mycoplasma hominis and high Waddlia seroprevalence |
| title_full_unstemmed | Human miscarriage and infection in Tunisia: Role of Mycoplasma hominis and high Waddlia seroprevalence |
| title_short | Human miscarriage and infection in Tunisia: Role of Mycoplasma hominis and high Waddlia seroprevalence |
| title_sort | human miscarriage and infection in tunisia role of mycoplasma hominis and high waddlia seroprevalence |
| topic | miscarriage genital infection zoonosis genital mycoplasmas Waddlia |
| url | https://jidc.org/index.php/journal/article/view/9829 |
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