A comparative study of the therapeutic effect of bone marrow mesenchymal stem cells versus insulin on mandibular dento-alveolar complex collagen formation and beta-catenin expression in experimentally induced type I diabetes
Objective: To assess and compare the therapeutic effect of bone marrow mesenchymal stem cells (BM-MSCs) versus insulin on mandibular dento-alveolar complex collagen formation and beta-catenin (β-catenin) expression in experimentally induced type I diabetes in albino rat. Design: Twenty-eight male al...
Saved in:
| Main Authors: | , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Springer
2023-09-01
|
| Series: | Saudi Dental Journal |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1013905223001025 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850245225349382144 |
|---|---|
| author | Rania Osama M. Mohsen Rabab Hassan |
| author_facet | Rania Osama M. Mohsen Rabab Hassan |
| author_sort | Rania Osama M. Mohsen |
| collection | DOAJ |
| description | Objective: To assess and compare the therapeutic effect of bone marrow mesenchymal stem cells (BM-MSCs) versus insulin on mandibular dento-alveolar complex collagen formation and beta-catenin (β-catenin) expression in experimentally induced type I diabetes in albino rat. Design: Twenty-eight male albino rats were equally divided as follows; Group I: was composed of rats which received no drug. The remaining rats were administrated a single streptozotocin (STZ) (40 mg/kg) intra-peritoneal injection. After affirmation of diabetes induction, the rats were divided into: Group II: Diabetic rats were given no treatment. Group III: Diabetic rats received a single BM-MSCs intravenous injection (1x106 cells). Group IV: Diabetic rats were given a daily insulin subcutaneous injection (5 IU/kg). After 28 days, mandibles were processed and stained by Hematoxylin & Eosin (H&E), Masson’s trichrome and anti-β-catenin antibody. A statistical analysis was performed to measure positive area% of Masson’s trichrome and β-catenin. Results: Dento-alveolar complex tissues and cells of Group II showed destructive changes histologically, while Groups III and IV demonstrated improved histological features. Group II presented almost old collagen in all dento-alveolar complex tissues, and nearly negative β-catenin expression. Groups III and IV revealed a newly formed collagen intermingled with very few areas of old collagen, and both groups showed positive β-catenin immunoreactivity. Statistically, Groups III and IV represented the highest mean values of Masson’s trichrome area% and β-catenin area%, while Group II reported the lowest mean. Conclusions: Streptozotocin has a destructive effect on the dento-alveolar complex structure and function. BM-MSCs and insulin show regenerative capacity in STZ-affected periodontal tissues, and statistically, they increase collagen formation and β-catenin expression. |
| format | Article |
| id | doaj-art-12294160be2c4159a37cfbd1bc0d66ff |
| institution | OA Journals |
| issn | 1013-9052 |
| language | English |
| publishDate | 2023-09-01 |
| publisher | Springer |
| record_format | Article |
| series | Saudi Dental Journal |
| spelling | doaj-art-12294160be2c4159a37cfbd1bc0d66ff2025-08-20T01:59:31ZengSpringerSaudi Dental Journal1013-90522023-09-0135666867710.1016/j.sdentj.2023.05.020A comparative study of the therapeutic effect of bone marrow mesenchymal stem cells versus insulin on mandibular dento-alveolar complex collagen formation and beta-catenin expression in experimentally induced type I diabetesRania Osama M. Mohsen0Rabab Hassan1Lecturer of Oral Biology, Faculty of Dentistry, Ain Shams University, Cairo, EgyptAssociate professor of Oral Biology, Faculty of Dentistry, Ain Shams University, Cairo, Egypt; Corresponding author at: Faculty of Dentistry, Ain-Shams University, Cairo, Egypt, Organization of African Unity St, Cairo, Egypt, Postal address: Inside Ain Shams University building, 11566, Cairo, Egypt.Objective: To assess and compare the therapeutic effect of bone marrow mesenchymal stem cells (BM-MSCs) versus insulin on mandibular dento-alveolar complex collagen formation and beta-catenin (β-catenin) expression in experimentally induced type I diabetes in albino rat. Design: Twenty-eight male albino rats were equally divided as follows; Group I: was composed of rats which received no drug. The remaining rats were administrated a single streptozotocin (STZ) (40 mg/kg) intra-peritoneal injection. After affirmation of diabetes induction, the rats were divided into: Group II: Diabetic rats were given no treatment. Group III: Diabetic rats received a single BM-MSCs intravenous injection (1x106 cells). Group IV: Diabetic rats were given a daily insulin subcutaneous injection (5 IU/kg). After 28 days, mandibles were processed and stained by Hematoxylin & Eosin (H&E), Masson’s trichrome and anti-β-catenin antibody. A statistical analysis was performed to measure positive area% of Masson’s trichrome and β-catenin. Results: Dento-alveolar complex tissues and cells of Group II showed destructive changes histologically, while Groups III and IV demonstrated improved histological features. Group II presented almost old collagen in all dento-alveolar complex tissues, and nearly negative β-catenin expression. Groups III and IV revealed a newly formed collagen intermingled with very few areas of old collagen, and both groups showed positive β-catenin immunoreactivity. Statistically, Groups III and IV represented the highest mean values of Masson’s trichrome area% and β-catenin area%, while Group II reported the lowest mean. Conclusions: Streptozotocin has a destructive effect on the dento-alveolar complex structure and function. BM-MSCs and insulin show regenerative capacity in STZ-affected periodontal tissues, and statistically, they increase collagen formation and β-catenin expression.http://www.sciencedirect.com/science/article/pii/S1013905223001025β-cateninBM-MSCsInsulinStreptozotocinDento-alveolar complex |
| spellingShingle | Rania Osama M. Mohsen Rabab Hassan A comparative study of the therapeutic effect of bone marrow mesenchymal stem cells versus insulin on mandibular dento-alveolar complex collagen formation and beta-catenin expression in experimentally induced type I diabetes Saudi Dental Journal β-catenin BM-MSCs Insulin Streptozotocin Dento-alveolar complex |
| title | A comparative study of the therapeutic effect of bone marrow mesenchymal stem cells versus insulin on mandibular dento-alveolar complex collagen formation and beta-catenin expression in experimentally induced type I diabetes |
| title_full | A comparative study of the therapeutic effect of bone marrow mesenchymal stem cells versus insulin on mandibular dento-alveolar complex collagen formation and beta-catenin expression in experimentally induced type I diabetes |
| title_fullStr | A comparative study of the therapeutic effect of bone marrow mesenchymal stem cells versus insulin on mandibular dento-alveolar complex collagen formation and beta-catenin expression in experimentally induced type I diabetes |
| title_full_unstemmed | A comparative study of the therapeutic effect of bone marrow mesenchymal stem cells versus insulin on mandibular dento-alveolar complex collagen formation and beta-catenin expression in experimentally induced type I diabetes |
| title_short | A comparative study of the therapeutic effect of bone marrow mesenchymal stem cells versus insulin on mandibular dento-alveolar complex collagen formation and beta-catenin expression in experimentally induced type I diabetes |
| title_sort | comparative study of the therapeutic effect of bone marrow mesenchymal stem cells versus insulin on mandibular dento alveolar complex collagen formation and beta catenin expression in experimentally induced type i diabetes |
| topic | β-catenin BM-MSCs Insulin Streptozotocin Dento-alveolar complex |
| url | http://www.sciencedirect.com/science/article/pii/S1013905223001025 |
| work_keys_str_mv | AT raniaosamammohsen acomparativestudyofthetherapeuticeffectofbonemarrowmesenchymalstemcellsversusinsulinonmandibulardentoalveolarcomplexcollagenformationandbetacateninexpressioninexperimentallyinducedtypeidiabetes AT rababhassan acomparativestudyofthetherapeuticeffectofbonemarrowmesenchymalstemcellsversusinsulinonmandibulardentoalveolarcomplexcollagenformationandbetacateninexpressioninexperimentallyinducedtypeidiabetes AT raniaosamammohsen comparativestudyofthetherapeuticeffectofbonemarrowmesenchymalstemcellsversusinsulinonmandibulardentoalveolarcomplexcollagenformationandbetacateninexpressioninexperimentallyinducedtypeidiabetes AT rababhassan comparativestudyofthetherapeuticeffectofbonemarrowmesenchymalstemcellsversusinsulinonmandibulardentoalveolarcomplexcollagenformationandbetacateninexpressioninexperimentallyinducedtypeidiabetes |