Cell free supernatants of Bifidobacterium adolescentis and Bifidobacterium longum suppress the tumor growth in colorectal cancer organoid model
Abstract The probiotic gut microbiome and its metabolites are pivotal in regulating host metabolism, inflammation, and immunity. Host genetics, colonization at birth, the host lifestyle, and exposure to diseases and drugs determine microbial composition. Dysbiosis and disruption of homeostasis in th...
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Nature Portfolio
2025-01-01
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| Series: | Scientific Reports |
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| Online Access: | https://doi.org/10.1038/s41598-024-83048-5 |
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| author | Min Jung Kim Myoung-Hyun Song Yo-Sep Ji Ji Won Park Young-Kyoung Shin Soon-Chan Kim Gihyeon Kim Beomki Cho Hansoo Park Ja-Lok Ku Seung-Yong Jeong |
| author_facet | Min Jung Kim Myoung-Hyun Song Yo-Sep Ji Ji Won Park Young-Kyoung Shin Soon-Chan Kim Gihyeon Kim Beomki Cho Hansoo Park Ja-Lok Ku Seung-Yong Jeong |
| author_sort | Min Jung Kim |
| collection | DOAJ |
| description | Abstract The probiotic gut microbiome and its metabolites are pivotal in regulating host metabolism, inflammation, and immunity. Host genetics, colonization at birth, the host lifestyle, and exposure to diseases and drugs determine microbial composition. Dysbiosis and disruption of homeostasis in the beneficial microbiome have been reported to be involved in the tumorigenesis and progression of colorectal cancer (CRC). However, the influence of bacteria-secreted metabolites on CRC growth is yet to be fully elucidated. In this study, we compared the microbial composition of CRC patients to healthy controls to identify distinct patterns of microbiota-derived metabolites in CRC patients. Metagenomic analysis demonstrated that beneficial bacteria strains; Blautia producta, Bifidobacterium adolescentis, and Bifidobacterium longum decreased, while Parabacteroides distasonis and Bacteroides ovatus were more prevalent in the CRC patient group. Treatment of cancer organoid lines with microbial culture supernatants from Blautia producta, Bifidobacterium adolescentis, and Bifidobacterium longum showed remarkable inhibition of cancer growth. This study demonstrates that the bacterial metabolites depleted in CRC patients may inhibit cancer growth and highlights the effects of microbiome-derived metabolites on CRC growth. |
| format | Article |
| id | doaj-art-121c2e17862f4d0b83a19f7d869eae72 |
| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj-art-121c2e17862f4d0b83a19f7d869eae722025-01-12T12:22:35ZengNature PortfolioScientific Reports2045-23222025-01-0115111810.1038/s41598-024-83048-5Cell free supernatants of Bifidobacterium adolescentis and Bifidobacterium longum suppress the tumor growth in colorectal cancer organoid modelMin Jung Kim0Myoung-Hyun Song1Yo-Sep Ji2Ji Won Park3Young-Kyoung Shin4Soon-Chan Kim5Gihyeon Kim6Beomki Cho7Hansoo Park8Ja-Lok Ku9Seung-Yong Jeong10Department of Surgery, Seoul National University College of MedicineCancer Research Institute, Seoul National UniversityHolzapfel Effective Microbes (HEM) Pharma, Handong Global UniversityDepartment of Surgery, Seoul National University College of MedicineCancer Research Institute, Seoul National UniversityCancer Research Institute, Seoul National UniversityDepartment of Biomedical Science and Engineering, Gwangju Institute of Science and Technology (GIST)Department of Biomedical Science and Engineering, Gwangju Institute of Science and Technology (GIST)Department of Biomedical Science and Engineering, Gwangju Institute of Science and Technology (GIST)Cancer Research Institute, Seoul National UniversityDepartment of Surgery, Seoul National University College of MedicineAbstract The probiotic gut microbiome and its metabolites are pivotal in regulating host metabolism, inflammation, and immunity. Host genetics, colonization at birth, the host lifestyle, and exposure to diseases and drugs determine microbial composition. Dysbiosis and disruption of homeostasis in the beneficial microbiome have been reported to be involved in the tumorigenesis and progression of colorectal cancer (CRC). However, the influence of bacteria-secreted metabolites on CRC growth is yet to be fully elucidated. In this study, we compared the microbial composition of CRC patients to healthy controls to identify distinct patterns of microbiota-derived metabolites in CRC patients. Metagenomic analysis demonstrated that beneficial bacteria strains; Blautia producta, Bifidobacterium adolescentis, and Bifidobacterium longum decreased, while Parabacteroides distasonis and Bacteroides ovatus were more prevalent in the CRC patient group. Treatment of cancer organoid lines with microbial culture supernatants from Blautia producta, Bifidobacterium adolescentis, and Bifidobacterium longum showed remarkable inhibition of cancer growth. This study demonstrates that the bacterial metabolites depleted in CRC patients may inhibit cancer growth and highlights the effects of microbiome-derived metabolites on CRC growth.https://doi.org/10.1038/s41598-024-83048-5Colorectal cancerMicrobiomeBifidobacteriumOrganoidMetabolite |
| spellingShingle | Min Jung Kim Myoung-Hyun Song Yo-Sep Ji Ji Won Park Young-Kyoung Shin Soon-Chan Kim Gihyeon Kim Beomki Cho Hansoo Park Ja-Lok Ku Seung-Yong Jeong Cell free supernatants of Bifidobacterium adolescentis and Bifidobacterium longum suppress the tumor growth in colorectal cancer organoid model Scientific Reports Colorectal cancer Microbiome Bifidobacterium Organoid Metabolite |
| title | Cell free supernatants of Bifidobacterium adolescentis and Bifidobacterium longum suppress the tumor growth in colorectal cancer organoid model |
| title_full | Cell free supernatants of Bifidobacterium adolescentis and Bifidobacterium longum suppress the tumor growth in colorectal cancer organoid model |
| title_fullStr | Cell free supernatants of Bifidobacterium adolescentis and Bifidobacterium longum suppress the tumor growth in colorectal cancer organoid model |
| title_full_unstemmed | Cell free supernatants of Bifidobacterium adolescentis and Bifidobacterium longum suppress the tumor growth in colorectal cancer organoid model |
| title_short | Cell free supernatants of Bifidobacterium adolescentis and Bifidobacterium longum suppress the tumor growth in colorectal cancer organoid model |
| title_sort | cell free supernatants of bifidobacterium adolescentis and bifidobacterium longum suppress the tumor growth in colorectal cancer organoid model |
| topic | Colorectal cancer Microbiome Bifidobacterium Organoid Metabolite |
| url | https://doi.org/10.1038/s41598-024-83048-5 |
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