Dentinal tubule penetration of sodium hypochlorite in root canals with and without mechanical preparation and different irrigant activation methods

ObjectivesThis study evaluated the dentinal penetration depth of 2.5% sodium hypochlorite (NaOCl) in root canals with and without preparation and different irrigant activation protocols.Materials and MethodsSixty-three bovine mandibular incisors were randomly allocated to 6 groups (n = 10): G1, prep...

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Main Authors: Renata Aqel de Oliveira, Theodoro Weissheimer, Gabriel Barcelos Só, Ricardo Abreu da Rosa, Matheus Albino Souza, Rodrigo Gonçalves Ribeiro, Marcus Vinicius Reis Só
Format: Article
Language:English
Published: Korean Academy of Conservative Dentistry 2023-02-01
Series:Restorative Dentistry & Endodontics
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Online Access:http://www.rde.ac/upload/pdf/rde-48-e1.pdf
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author Renata Aqel de Oliveira
Theodoro Weissheimer
Gabriel Barcelos Só
Ricardo Abreu da Rosa
Matheus Albino Souza
Rodrigo Gonçalves Ribeiro
Marcus Vinicius Reis Só
author_facet Renata Aqel de Oliveira
Theodoro Weissheimer
Gabriel Barcelos Só
Ricardo Abreu da Rosa
Matheus Albino Souza
Rodrigo Gonçalves Ribeiro
Marcus Vinicius Reis Só
author_sort Renata Aqel de Oliveira
collection DOAJ
description ObjectivesThis study evaluated the dentinal penetration depth of 2.5% sodium hypochlorite (NaOCl) in root canals with and without preparation and different irrigant activation protocols.Materials and MethodsSixty-three bovine mandibular incisors were randomly allocated to 6 groups (n = 10): G1, preparation + conventional needle irrigation (CNI); G2, preparation + passive ultrasonic irrigation (PUI); G3, preparation + Odous Clean (OC); G4, no preparation + CNI; G5, no preparation + PUI; G6, no preparation + OC; and CG (negative control; n = 3). Samples were filled with crystal violet for 72 hours. Irrigant activation was performed. Samples were sectioned perpendicularly along the long axis, 3 mm and 7 mm from the apex. Images of the root thirds of each block were captured with a stereomicroscope and analyzed with an image analysis software. One-way analysis of variance, followed by the Tukey post hoc test, and the Student’s t-test were used for data analysis, with a significance level of 5%.ResultsThe NaOCl penetration depth was similar when preparation was performed, regardless of the method of irrigation activation (p > 0.05). In the groups without preparation, G6 showed greater NaOCl penetration depth (p < 0.05). The groups without preparation had a greater NaOCl penetration depth than those with preparation (p = 0.0019).ConclusionsThe NaOCl penetration depth was similar in groups with root canal preparation. Without root canal preparation, OC allowed deeper NaOCl penetration. The groups without preparation had greater NaOCl penetration than those undergoing root canal preparation.
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publishDate 2023-02-01
publisher Korean Academy of Conservative Dentistry
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spelling doaj-art-12198b3b277d4176a765f4fdcd679eda2025-08-20T03:15:03ZengKorean Academy of Conservative DentistryRestorative Dentistry & Endodontics2234-76582234-76662023-02-01481e110.5395/rde.2023.48.e11050Dentinal tubule penetration of sodium hypochlorite in root canals with and without mechanical preparation and different irrigant activation methodsRenata Aqel de Oliveira0Theodoro Weissheimer1Gabriel Barcelos Só2Ricardo Abreu da Rosa3Matheus Albino Souza4Rodrigo Gonçalves Ribeiro5Marcus Vinicius Reis Só6Department of Conservative Dentistry, School of Dentistry, Federal University of Rio Grande do Sul (UFRGS), Porto Alegre, RS, BrazilDepartment of Conservative Dentistry, School of Dentistry, Federal University of Rio Grande do Sul (UFRGS), Porto Alegre, RS, BrazilDepartment of Conservative Dentistry, School of Dentistry, Federal University of Rio Grande do Sul (UFRGS), Porto Alegre, RS, BrazilDepartment of Conservative Dentistry, School of Dentistry, Federal University of Rio Grande do Sul (UFRGS), Porto Alegre, RS, BrazilDepartment of Endodontics, School of Dentistry, University of Passo Fundo (UPF), Passo Fundo, RS, BrazilDepartment of Endodontics, School of Dentistry, State University of Western Paraná (UNIOESTE), Cascavel, PR, BrazilDepartment of Conservative Dentistry, School of Dentistry, Federal University of Rio Grande do Sul (UFRGS), Porto Alegre, RS, BrazilObjectivesThis study evaluated the dentinal penetration depth of 2.5% sodium hypochlorite (NaOCl) in root canals with and without preparation and different irrigant activation protocols.Materials and MethodsSixty-three bovine mandibular incisors were randomly allocated to 6 groups (n = 10): G1, preparation + conventional needle irrigation (CNI); G2, preparation + passive ultrasonic irrigation (PUI); G3, preparation + Odous Clean (OC); G4, no preparation + CNI; G5, no preparation + PUI; G6, no preparation + OC; and CG (negative control; n = 3). Samples were filled with crystal violet for 72 hours. Irrigant activation was performed. Samples were sectioned perpendicularly along the long axis, 3 mm and 7 mm from the apex. Images of the root thirds of each block were captured with a stereomicroscope and analyzed with an image analysis software. One-way analysis of variance, followed by the Tukey post hoc test, and the Student’s t-test were used for data analysis, with a significance level of 5%.ResultsThe NaOCl penetration depth was similar when preparation was performed, regardless of the method of irrigation activation (p > 0.05). In the groups without preparation, G6 showed greater NaOCl penetration depth (p < 0.05). The groups without preparation had a greater NaOCl penetration depth than those with preparation (p = 0.0019).ConclusionsThe NaOCl penetration depth was similar in groups with root canal preparation. Without root canal preparation, OC allowed deeper NaOCl penetration. The groups without preparation had greater NaOCl penetration than those undergoing root canal preparation.http://www.rde.ac/upload/pdf/rde-48-e1.pdfdentin penetrationendodonticregenerative endodonticsmear layersodium hypochlorite
spellingShingle Renata Aqel de Oliveira
Theodoro Weissheimer
Gabriel Barcelos Só
Ricardo Abreu da Rosa
Matheus Albino Souza
Rodrigo Gonçalves Ribeiro
Marcus Vinicius Reis Só
Dentinal tubule penetration of sodium hypochlorite in root canals with and without mechanical preparation and different irrigant activation methods
Restorative Dentistry & Endodontics
dentin penetration
endodontic
regenerative endodontic
smear layer
sodium hypochlorite
title Dentinal tubule penetration of sodium hypochlorite in root canals with and without mechanical preparation and different irrigant activation methods
title_full Dentinal tubule penetration of sodium hypochlorite in root canals with and without mechanical preparation and different irrigant activation methods
title_fullStr Dentinal tubule penetration of sodium hypochlorite in root canals with and without mechanical preparation and different irrigant activation methods
title_full_unstemmed Dentinal tubule penetration of sodium hypochlorite in root canals with and without mechanical preparation and different irrigant activation methods
title_short Dentinal tubule penetration of sodium hypochlorite in root canals with and without mechanical preparation and different irrigant activation methods
title_sort dentinal tubule penetration of sodium hypochlorite in root canals with and without mechanical preparation and different irrigant activation methods
topic dentin penetration
endodontic
regenerative endodontic
smear layer
sodium hypochlorite
url http://www.rde.ac/upload/pdf/rde-48-e1.pdf
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