Effect of fecal microbiota transplantation on ulcerative colitis model in rats: The gut–brain axis
Study objectives: The impact of fecal microbiota transplantation (FMT) on the TLR4/MYD88/NF-kB signaling pathway in the colon in the ulcerative colitis model, as well as the incidence of anxiety behaviors caused by the colitis model was investigated. Methods: Twenthy four ats were induced with ulcer...
Saved in:
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2025-02-01
|
| Series: | Heliyon |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405844025008102 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1825206846086971392 |
|---|---|
| author | Mahdis Morshedbak Kaveh Rahimi Mohammad Reza Tabandeh |
| author_facet | Mahdis Morshedbak Kaveh Rahimi Mohammad Reza Tabandeh |
| author_sort | Mahdis Morshedbak |
| collection | DOAJ |
| description | Study objectives: The impact of fecal microbiota transplantation (FMT) on the TLR4/MYD88/NF-kB signaling pathway in the colon in the ulcerative colitis model, as well as the incidence of anxiety behaviors caused by the colitis model was investigated. Methods: Twenthy four ats were induced with ulcerative colitis using a 4 % acetic acid solution administered intrarectally and were subsequently treated with prednisolone and FMT. The study examined several indicators, such as TLR4, MYD88, and NF-κB mRNA expression, along with oxidative stress factors. Additionally, it examined the relationship between anxiety-related behaviors and colitis and assessed the pro-inflammatory cytokines in the hippocampus. Results: FMT led to lower disease score index and improved colon tissue pathology findings. This was associated with reduced mRNA expression of TLR4, MYD88, and NF-κB, as well as lower levels of TOS, and higher levels of TAC, GSH, and GSSG in colon tissues. FMT was found to reduce anxiety in both the open field and elevated plus maze tests. Additionally, levels of IL-6 and TNF-a were decreased in the hippocampus. Conclusions: FMT suppressed acetic acid-induced colitis by inhibiting the TLR4/MYD88/NF-kB signaling pathway. FMT reduced anxiety in open field and plus maze tests, and resulted in decreased levels of IL-6 and TNF-a in the hippocampus. |
| format | Article |
| id | doaj-art-1216127af4ac4a76a1056539a44cf809 |
| institution | Kabale University |
| issn | 2405-8440 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Heliyon |
| spelling | doaj-art-1216127af4ac4a76a1056539a44cf8092025-02-07T04:47:57ZengElsevierHeliyon2405-84402025-02-01113e42430Effect of fecal microbiota transplantation on ulcerative colitis model in rats: The gut–brain axisMahdis Morshedbak0Kaveh Rahimi1Mohammad Reza Tabandeh2Department of Basic Sciences, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, IranDepartment of Basic Sciences, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran; Corresponding author.Department of Basic Sciences, Division of Biochemistry and Molecular Biology, Faculty of Veterinary Medicine, Shahid Chamran Univeristy of Ahvaz, Ahvaz, Iran; Stem Cells and Transgenic Technology Research Center, Shahid Chamran University of Ahvaz, Ahvaz, IranStudy objectives: The impact of fecal microbiota transplantation (FMT) on the TLR4/MYD88/NF-kB signaling pathway in the colon in the ulcerative colitis model, as well as the incidence of anxiety behaviors caused by the colitis model was investigated. Methods: Twenthy four ats were induced with ulcerative colitis using a 4 % acetic acid solution administered intrarectally and were subsequently treated with prednisolone and FMT. The study examined several indicators, such as TLR4, MYD88, and NF-κB mRNA expression, along with oxidative stress factors. Additionally, it examined the relationship between anxiety-related behaviors and colitis and assessed the pro-inflammatory cytokines in the hippocampus. Results: FMT led to lower disease score index and improved colon tissue pathology findings. This was associated with reduced mRNA expression of TLR4, MYD88, and NF-κB, as well as lower levels of TOS, and higher levels of TAC, GSH, and GSSG in colon tissues. FMT was found to reduce anxiety in both the open field and elevated plus maze tests. Additionally, levels of IL-6 and TNF-a were decreased in the hippocampus. Conclusions: FMT suppressed acetic acid-induced colitis by inhibiting the TLR4/MYD88/NF-kB signaling pathway. FMT reduced anxiety in open field and plus maze tests, and resulted in decreased levels of IL-6 and TNF-a in the hippocampus.http://www.sciencedirect.com/science/article/pii/S2405844025008102Fecal microbiota transplantationUlcerative colitisGut–brain axis |
| spellingShingle | Mahdis Morshedbak Kaveh Rahimi Mohammad Reza Tabandeh Effect of fecal microbiota transplantation on ulcerative colitis model in rats: The gut–brain axis Heliyon Fecal microbiota transplantation Ulcerative colitis Gut–brain axis |
| title | Effect of fecal microbiota transplantation on ulcerative colitis model in rats: The gut–brain axis |
| title_full | Effect of fecal microbiota transplantation on ulcerative colitis model in rats: The gut–brain axis |
| title_fullStr | Effect of fecal microbiota transplantation on ulcerative colitis model in rats: The gut–brain axis |
| title_full_unstemmed | Effect of fecal microbiota transplantation on ulcerative colitis model in rats: The gut–brain axis |
| title_short | Effect of fecal microbiota transplantation on ulcerative colitis model in rats: The gut–brain axis |
| title_sort | effect of fecal microbiota transplantation on ulcerative colitis model in rats the gut brain axis |
| topic | Fecal microbiota transplantation Ulcerative colitis Gut–brain axis |
| url | http://www.sciencedirect.com/science/article/pii/S2405844025008102 |
| work_keys_str_mv | AT mahdismorshedbak effectoffecalmicrobiotatransplantationonulcerativecolitismodelinratsthegutbrainaxis AT kavehrahimi effectoffecalmicrobiotatransplantationonulcerativecolitismodelinratsthegutbrainaxis AT mohammadrezatabandeh effectoffecalmicrobiotatransplantationonulcerativecolitismodelinratsthegutbrainaxis |