Microscopic Photosensitization: A New Tool to Investigate the Role of Mitochondria in Cell Death
Active involvement of mitochondria in cell death has been well-documented, but local apoptotic signaling between subsets of mitochondria has been poorly explored to date. Using mitochondrially localized CMXRos as a photosensitizer coupled to laser irradiation by confocal laser scanning microscopy, w...
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| Format: | Article |
| Language: | English |
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Wiley
2002-01-01
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| Series: | The Scientific World Journal |
| Online Access: | http://dx.doi.org/10.1100/tsw.2002.227 |
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| author | May-Ghee Lum Tetsuhiro Minamikawa Phillip Nagley |
| author_facet | May-Ghee Lum Tetsuhiro Minamikawa Phillip Nagley |
| author_sort | May-Ghee Lum |
| collection | DOAJ |
| description | Active involvement of mitochondria in cell death has been well-documented, but local apoptotic signaling between subsets of mitochondria has been poorly explored to date. Using mitochondrially localized CMXRos as a photosensitizer coupled to laser irradiation by confocal laser scanning microscopy, we demonstrate that partial irradiation of about half the mitochondria in human 143B TK– cells induces rapid loss of mitochondrial membrane potential (ΔΨm) in nonirradiated mitochondria. Cells so partially irradiated show apoptotic indications, including mobilization of cytochrome c and binding of annexin V within 2 h following irradiation. The loss of ΔΨm in nonirradiated mitochondria did not occur in cells photoirradiated in the absence of CMXRos. Increasing the proportion of irradiated mitochondria in each cell (up to about 50%) generated a correspondingly greater percentage of cells in which nonirradiated mitochondria lost ΔΨm and which also showed apoptotic indications. Only at the highest level of irradiation (global for all mitochondria in one cell) were signs of necrosis evident (judged by uptake of propidium iodide). Because laser irradiation is specific to the subpopulation of mitochondria targeted, the data imply that a signal emanating from irradiated mitochondria is processed by their nonirradiated counterparts. We conclude that intermitochondrial signaling occurs in the subcellular response to induction of apoptosis. |
| format | Article |
| id | doaj-art-1214a1cf383a4678adee295e3abebfbb |
| institution | Kabale University |
| issn | 1537-744X |
| language | English |
| publishDate | 2002-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | The Scientific World Journal |
| spelling | doaj-art-1214a1cf383a4678adee295e3abebfbb2025-02-03T01:31:03ZengWileyThe Scientific World Journal1537-744X2002-01-0121198120810.1100/tsw.2002.227Microscopic Photosensitization: A New Tool to Investigate the Role of Mitochondria in Cell DeathMay-Ghee Lum0Tetsuhiro Minamikawa1Phillip Nagley2Department of Biochemistry and Molecular Biology, PO Box 13D, Monash University, Victoria 3800, AustraliaOlympus Australia Pty. Ltd., 1/104 Ferntree Gully Rd., Oakleigh VIC 3166, AustraliaDepartment of Biochemistry and Molecular Biology, PO Box 13D, Monash University, Victoria 3800, AustraliaActive involvement of mitochondria in cell death has been well-documented, but local apoptotic signaling between subsets of mitochondria has been poorly explored to date. Using mitochondrially localized CMXRos as a photosensitizer coupled to laser irradiation by confocal laser scanning microscopy, we demonstrate that partial irradiation of about half the mitochondria in human 143B TK– cells induces rapid loss of mitochondrial membrane potential (ΔΨm) in nonirradiated mitochondria. Cells so partially irradiated show apoptotic indications, including mobilization of cytochrome c and binding of annexin V within 2 h following irradiation. The loss of ΔΨm in nonirradiated mitochondria did not occur in cells photoirradiated in the absence of CMXRos. Increasing the proportion of irradiated mitochondria in each cell (up to about 50%) generated a correspondingly greater percentage of cells in which nonirradiated mitochondria lost ΔΨm and which also showed apoptotic indications. Only at the highest level of irradiation (global for all mitochondria in one cell) were signs of necrosis evident (judged by uptake of propidium iodide). Because laser irradiation is specific to the subpopulation of mitochondria targeted, the data imply that a signal emanating from irradiated mitochondria is processed by their nonirradiated counterparts. We conclude that intermitochondrial signaling occurs in the subcellular response to induction of apoptosis.http://dx.doi.org/10.1100/tsw.2002.227 |
| spellingShingle | May-Ghee Lum Tetsuhiro Minamikawa Phillip Nagley Microscopic Photosensitization: A New Tool to Investigate the Role of Mitochondria in Cell Death The Scientific World Journal |
| title | Microscopic Photosensitization: A New Tool to Investigate the Role of Mitochondria in Cell Death |
| title_full | Microscopic Photosensitization: A New Tool to Investigate the Role of Mitochondria in Cell Death |
| title_fullStr | Microscopic Photosensitization: A New Tool to Investigate the Role of Mitochondria in Cell Death |
| title_full_unstemmed | Microscopic Photosensitization: A New Tool to Investigate the Role of Mitochondria in Cell Death |
| title_short | Microscopic Photosensitization: A New Tool to Investigate the Role of Mitochondria in Cell Death |
| title_sort | microscopic photosensitization a new tool to investigate the role of mitochondria in cell death |
| url | http://dx.doi.org/10.1100/tsw.2002.227 |
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