Halomonas sp. MC140, a polyhydroxyalkanoate (PHA) producer isolated from the Arctic environment
Abstract Polyhydroxyalkanoates (PHA) are biobased and biodegradable polyesters with potential to replace petroleum-based plastics or for use as feed in aquaculture. In this study, Halomonas sp. MC140 was isolated from the Arctic littoral environment in Norway and characterized in terms of PHA produc...
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Nature Portfolio
2025-07-01
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| Online Access: | https://doi.org/10.1038/s41598-025-06898-7 |
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| author | Mikkel Christensen Iulia Chiciudean Irina Lascu Piotr Jablonski Volha Shapaval Boris Zimmermann Ana-Maria Tanase Hilde Hansen |
| author_facet | Mikkel Christensen Iulia Chiciudean Irina Lascu Piotr Jablonski Volha Shapaval Boris Zimmermann Ana-Maria Tanase Hilde Hansen |
| author_sort | Mikkel Christensen |
| collection | DOAJ |
| description | Abstract Polyhydroxyalkanoates (PHA) are biobased and biodegradable polyesters with potential to replace petroleum-based plastics or for use as feed in aquaculture. In this study, Halomonas sp. MC140 was isolated from the Arctic littoral environment in Norway and characterized in terms of PHA production. Phylogenetic analysis of the draft genome sequence placed the strain as closely related to Halomonas profundi MT13. A genome-based model of PHA biosynthesis pathways was constructed, and shake-flask experiments identified the highest PHA production in the form of polyhydroxybutyrate (PHB) with acetate (35 ± 4.8%) or glucose (28 ± 7.9%) supplementation, as verified by gas chromatography-flame ionization detector (GC-FID) analysis, while the addition of propionate produced PHB-co-valerate (PHBV) co-polymer (≈ 1% 3-HV). Low PHB production (< 5%) was found from supplementation with glycerol or fructose. A multivariate Partial Least Squares Regression (PLSR) model for low- to medium-range (0–40%) PHB content in Halomonas sp. MC140 was constructed with Fourier transform infrared spectroscopy (FTIR) spectra and used to assess PHA production over time. Additionally, the strain’s carbon substrate utilization phenotype was characterized using the Biolog assay. These findings suggest that PHA production by Halomonas sp. MC140 is modest compared to other Halomonas species but provides a starting point for optimization or use in sustainable feed applications in Norwegian aquaculture, where lower PHA production is sufficient compared to bioplastic production. |
| format | Article |
| id | doaj-art-120a551dfa3e4d4e877e5d33f5e6ae85 |
| institution | DOAJ |
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| language | English |
| publishDate | 2025-07-01 |
| publisher | Nature Portfolio |
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| spelling | doaj-art-120a551dfa3e4d4e877e5d33f5e6ae852025-08-20T03:05:18ZengNature PortfolioScientific Reports2045-23222025-07-0115111410.1038/s41598-025-06898-7Halomonas sp. MC140, a polyhydroxyalkanoate (PHA) producer isolated from the Arctic environmentMikkel Christensen0Iulia Chiciudean1Irina Lascu2Piotr Jablonski3Volha Shapaval4Boris Zimmermann5Ana-Maria Tanase6Hilde Hansen7Department of Chemistry, UiT The Arctic University of NorwayDepartment of Genetics, Faculty of Biology, University of BucharestDepartment of Genetics, Faculty of Biology, University of BucharestDepartment of Chemistry, Umeå UniversityFaculty of Science and Technology, Norwegian University of Life SciencesFaculty of Science and Technology, Norwegian University of Life SciencesDepartment of Genetics, Faculty of Biology, University of BucharestDepartment of Chemistry, UiT The Arctic University of NorwayAbstract Polyhydroxyalkanoates (PHA) are biobased and biodegradable polyesters with potential to replace petroleum-based plastics or for use as feed in aquaculture. In this study, Halomonas sp. MC140 was isolated from the Arctic littoral environment in Norway and characterized in terms of PHA production. Phylogenetic analysis of the draft genome sequence placed the strain as closely related to Halomonas profundi MT13. A genome-based model of PHA biosynthesis pathways was constructed, and shake-flask experiments identified the highest PHA production in the form of polyhydroxybutyrate (PHB) with acetate (35 ± 4.8%) or glucose (28 ± 7.9%) supplementation, as verified by gas chromatography-flame ionization detector (GC-FID) analysis, while the addition of propionate produced PHB-co-valerate (PHBV) co-polymer (≈ 1% 3-HV). Low PHB production (< 5%) was found from supplementation with glycerol or fructose. A multivariate Partial Least Squares Regression (PLSR) model for low- to medium-range (0–40%) PHB content in Halomonas sp. MC140 was constructed with Fourier transform infrared spectroscopy (FTIR) spectra and used to assess PHA production over time. Additionally, the strain’s carbon substrate utilization phenotype was characterized using the Biolog assay. These findings suggest that PHA production by Halomonas sp. MC140 is modest compared to other Halomonas species but provides a starting point for optimization or use in sustainable feed applications in Norwegian aquaculture, where lower PHA production is sufficient compared to bioplastic production.https://doi.org/10.1038/s41598-025-06898-7PHAPolyhydroxyalkanoatesPHBPHBVHalomonasBiolog |
| spellingShingle | Mikkel Christensen Iulia Chiciudean Irina Lascu Piotr Jablonski Volha Shapaval Boris Zimmermann Ana-Maria Tanase Hilde Hansen Halomonas sp. MC140, a polyhydroxyalkanoate (PHA) producer isolated from the Arctic environment Scientific Reports PHA Polyhydroxyalkanoates PHB PHBV Halomonas Biolog |
| title | Halomonas sp. MC140, a polyhydroxyalkanoate (PHA) producer isolated from the Arctic environment |
| title_full | Halomonas sp. MC140, a polyhydroxyalkanoate (PHA) producer isolated from the Arctic environment |
| title_fullStr | Halomonas sp. MC140, a polyhydroxyalkanoate (PHA) producer isolated from the Arctic environment |
| title_full_unstemmed | Halomonas sp. MC140, a polyhydroxyalkanoate (PHA) producer isolated from the Arctic environment |
| title_short | Halomonas sp. MC140, a polyhydroxyalkanoate (PHA) producer isolated from the Arctic environment |
| title_sort | halomonas sp mc140 a polyhydroxyalkanoate pha producer isolated from the arctic environment |
| topic | PHA Polyhydroxyalkanoates PHB PHBV Halomonas Biolog |
| url | https://doi.org/10.1038/s41598-025-06898-7 |
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