Curcumin Nanocarriers in the Protection Against Iron- and Alcohol-Induced Oxidative Stress in a Cellular Model of Liver Disease
During chronic alcohol misuse, hepatic iron overload occurs, leading to exacerbated oxidative stress and liver injury. The aim was to study formulations encapsulated with the antioxidant curcumin to assess their ability protect against oxidative stress in a model of alcohol-related liver disease (AL...
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MDPI AG
2025-04-01
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| author | Lucy Petagine Mohammed G. Zariwala Satyanarayana Somavarapu Stefanie Ho Yi Chan Vinood B. Patel |
| author_facet | Lucy Petagine Mohammed G. Zariwala Satyanarayana Somavarapu Stefanie Ho Yi Chan Vinood B. Patel |
| author_sort | Lucy Petagine |
| collection | DOAJ |
| description | During chronic alcohol misuse, hepatic iron overload occurs, leading to exacerbated oxidative stress and liver injury. The aim was to study formulations encapsulated with the antioxidant curcumin to assess their ability protect against oxidative stress in a model of alcohol-related liver disease (ALD) combined with iron. HepG2 (VL-17A) cells were treated with iron (50 µM) alone or with alcohol (200 to 350 mM) over 72 h and markers of oxidative damage, cell death, and mitochondrial function were assessed. Nanoformulations encapsulating curcumin were also studied. VL-17A cells treated with both ethanol and iron showed significant decreases in cell viability (64%, <i>p</i> < 0.0001) when compared to control, and a 56% decrease (<i>p</i> = 0.0279) when compared to iron-only treatment. Iron-alone treatment caused a 115% increase (<i>p</i> < 0.0001) in ROS at 48 h as well as increases of up to 118% when treated with 200 mM ethanol + 50 μM iron (<i>p</i> < 0.0001), compared to control DMEM. The study found that 10 µM curcumin DSPE-PEG increased cell viability by 17% and 41% when compared to control and iron treatment alone, respectively. Formulations reduced ROS by 36% (<i>p</i> = 0.0015) when compared to iron-alone treatment. In summary, encapsulated curcumin provided antioxidant capacity and reduced oxidative stress, demonstrating the therapeutic potential for curcumin formulations in ALD combined with iron dysregulation. |
| format | Article |
| id | doaj-art-1208afcf2b28445187d635ceeede78cf |
| institution | DOAJ |
| issn | 2079-7737 |
| language | English |
| publishDate | 2025-04-01 |
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| spelling | doaj-art-1208afcf2b28445187d635ceeede78cf2025-08-20T03:14:40ZengMDPI AGBiology2079-77372025-04-0114545510.3390/biology14050455Curcumin Nanocarriers in the Protection Against Iron- and Alcohol-Induced Oxidative Stress in a Cellular Model of Liver DiseaseLucy Petagine0Mohammed G. Zariwala1Satyanarayana Somavarapu2Stefanie Ho Yi Chan3Vinood B. Patel4Centre for Nutraceuticals, School of Life Sciences, University of Westminster, London W1W 6UW, UKCentre for Nutraceuticals, School of Life Sciences, University of Westminster, London W1W 6UW, UKDepartment of Pharmaceutics, UCL School of Pharmacy, London WC1N 1AX, UKCentre for Nutraceuticals, School of Life Sciences, University of Westminster, London W1W 6UW, UKCentre for Nutraceuticals, School of Life Sciences, University of Westminster, London W1W 6UW, UKDuring chronic alcohol misuse, hepatic iron overload occurs, leading to exacerbated oxidative stress and liver injury. The aim was to study formulations encapsulated with the antioxidant curcumin to assess their ability protect against oxidative stress in a model of alcohol-related liver disease (ALD) combined with iron. HepG2 (VL-17A) cells were treated with iron (50 µM) alone or with alcohol (200 to 350 mM) over 72 h and markers of oxidative damage, cell death, and mitochondrial function were assessed. Nanoformulations encapsulating curcumin were also studied. VL-17A cells treated with both ethanol and iron showed significant decreases in cell viability (64%, <i>p</i> < 0.0001) when compared to control, and a 56% decrease (<i>p</i> = 0.0279) when compared to iron-only treatment. Iron-alone treatment caused a 115% increase (<i>p</i> < 0.0001) in ROS at 48 h as well as increases of up to 118% when treated with 200 mM ethanol + 50 μM iron (<i>p</i> < 0.0001), compared to control DMEM. The study found that 10 µM curcumin DSPE-PEG increased cell viability by 17% and 41% when compared to control and iron treatment alone, respectively. Formulations reduced ROS by 36% (<i>p</i> = 0.0015) when compared to iron-alone treatment. In summary, encapsulated curcumin provided antioxidant capacity and reduced oxidative stress, demonstrating the therapeutic potential for curcumin formulations in ALD combined with iron dysregulation.https://www.mdpi.com/2079-7737/14/5/455alcoholantioxidantscurcuminironlivermitochondria |
| spellingShingle | Lucy Petagine Mohammed G. Zariwala Satyanarayana Somavarapu Stefanie Ho Yi Chan Vinood B. Patel Curcumin Nanocarriers in the Protection Against Iron- and Alcohol-Induced Oxidative Stress in a Cellular Model of Liver Disease Biology alcohol antioxidants curcumin iron liver mitochondria |
| title | Curcumin Nanocarriers in the Protection Against Iron- and Alcohol-Induced Oxidative Stress in a Cellular Model of Liver Disease |
| title_full | Curcumin Nanocarriers in the Protection Against Iron- and Alcohol-Induced Oxidative Stress in a Cellular Model of Liver Disease |
| title_fullStr | Curcumin Nanocarriers in the Protection Against Iron- and Alcohol-Induced Oxidative Stress in a Cellular Model of Liver Disease |
| title_full_unstemmed | Curcumin Nanocarriers in the Protection Against Iron- and Alcohol-Induced Oxidative Stress in a Cellular Model of Liver Disease |
| title_short | Curcumin Nanocarriers in the Protection Against Iron- and Alcohol-Induced Oxidative Stress in a Cellular Model of Liver Disease |
| title_sort | curcumin nanocarriers in the protection against iron and alcohol induced oxidative stress in a cellular model of liver disease |
| topic | alcohol antioxidants curcumin iron liver mitochondria |
| url | https://www.mdpi.com/2079-7737/14/5/455 |
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