Plasma Levels of t-PA and PAI-1 Correlate With the Formation of Experimental Post-Surgical Peritoneal Adhesions

This study has evaluated whether systemic changes of plasminogen activator (t-PA) and plasminogen activator inhibitor-1 (PAI-1) parallel the adhesions development and whether they could be used as predictors of adhesion risk. This has been studied in an animal model of post-surgical peritoneal adh...

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Main Authors: Clara Di Filippo, Alessandro Falsetto, Vito De Pascale, Elisabetta Tufariello, Domenico De Lucia, Francesco Rossi, Michele D'Amico, Antonio Cennamo
Format: Article
Language:English
Published: Wiley 2006-01-01
Series:Mediators of Inflammation
Online Access:http://dx.doi.org/10.1155/MI/2006/13901
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author Clara Di Filippo
Alessandro Falsetto
Vito De Pascale
Elisabetta Tufariello
Domenico De Lucia
Francesco Rossi
Michele D'Amico
Antonio Cennamo
author_facet Clara Di Filippo
Alessandro Falsetto
Vito De Pascale
Elisabetta Tufariello
Domenico De Lucia
Francesco Rossi
Michele D'Amico
Antonio Cennamo
author_sort Clara Di Filippo
collection DOAJ
description This study has evaluated whether systemic changes of plasminogen activator (t-PA) and plasminogen activator inhibitor-1 (PAI-1) parallel the adhesions development and whether they could be used as predictors of adhesion risk. This has been studied in an animal model of post-surgical peritoneal adhesion by monitoring for 10 days the plasma and tissue levels of t-PA and PAI-1. The results showed that both tissular and plasmatic levels of t-PA were decreased in concomitance with the development of peritoneal adhesions. In contrast, PAI-1 was found increased into the tissue and into the plasma samples of the rats taken at 5 and 10 days time points. Inflammatory mediators such as ICAM-1, VCAM-1, and IL-6 within the peritoneal lavage fluid also correlated with the adhesion formation process. In conclusion, post-surgical peritoneal adhesions provide alterations of local inflammatory components and local and systemic fibrinolytic components, possibly with PAI-1 quenching t-PA. This may have potential for the identification of high-risk patients.
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series Mediators of Inflammation
spelling doaj-art-1206de40b2f14643a6cc76914072c45a2025-08-20T02:01:54ZengWileyMediators of Inflammation0962-93511466-18612006-01-01200610.1155/MI/2006/1390113901Plasma Levels of t-PA and PAI-1 Correlate With the Formation of Experimental Post-Surgical Peritoneal AdhesionsClara Di Filippo0Alessandro Falsetto1Vito De Pascale2Elisabetta Tufariello3Domenico De Lucia4Francesco Rossi5Michele D'Amico6Antonio Cennamo7Section of Pharmacology L. Donatelli, Department of Experimental Medicine, Second University of Naples, Naples 80138, ItalyDepartment of Anaesthetic Sciences and Emergency Surgery, Second University of Naples, Naples 80138, ItalyDepartment of Anaesthetic Sciences and Emergency Surgery, Second University of Naples, Naples 80138, ItalySection of Pharmacology L. Donatelli, Department of Experimental Medicine, Second University of Naples, Naples 80138, ItalyLaboratory of Hemostasis and Thrombosis, Second University of Naples, Naples 80138, ItalySection of Pharmacology L. Donatelli, Department of Experimental Medicine, Second University of Naples, Naples 80138, ItalySection of Pharmacology L. Donatelli, Department of Experimental Medicine, Second University of Naples, Naples 80138, ItalyDepartment of Anaesthetic Sciences and Emergency Surgery, Second University of Naples, Naples 80138, ItalyThis study has evaluated whether systemic changes of plasminogen activator (t-PA) and plasminogen activator inhibitor-1 (PAI-1) parallel the adhesions development and whether they could be used as predictors of adhesion risk. This has been studied in an animal model of post-surgical peritoneal adhesion by monitoring for 10 days the plasma and tissue levels of t-PA and PAI-1. The results showed that both tissular and plasmatic levels of t-PA were decreased in concomitance with the development of peritoneal adhesions. In contrast, PAI-1 was found increased into the tissue and into the plasma samples of the rats taken at 5 and 10 days time points. Inflammatory mediators such as ICAM-1, VCAM-1, and IL-6 within the peritoneal lavage fluid also correlated with the adhesion formation process. In conclusion, post-surgical peritoneal adhesions provide alterations of local inflammatory components and local and systemic fibrinolytic components, possibly with PAI-1 quenching t-PA. This may have potential for the identification of high-risk patients.http://dx.doi.org/10.1155/MI/2006/13901
spellingShingle Clara Di Filippo
Alessandro Falsetto
Vito De Pascale
Elisabetta Tufariello
Domenico De Lucia
Francesco Rossi
Michele D'Amico
Antonio Cennamo
Plasma Levels of t-PA and PAI-1 Correlate With the Formation of Experimental Post-Surgical Peritoneal Adhesions
Mediators of Inflammation
title Plasma Levels of t-PA and PAI-1 Correlate With the Formation of Experimental Post-Surgical Peritoneal Adhesions
title_full Plasma Levels of t-PA and PAI-1 Correlate With the Formation of Experimental Post-Surgical Peritoneal Adhesions
title_fullStr Plasma Levels of t-PA and PAI-1 Correlate With the Formation of Experimental Post-Surgical Peritoneal Adhesions
title_full_unstemmed Plasma Levels of t-PA and PAI-1 Correlate With the Formation of Experimental Post-Surgical Peritoneal Adhesions
title_short Plasma Levels of t-PA and PAI-1 Correlate With the Formation of Experimental Post-Surgical Peritoneal Adhesions
title_sort plasma levels of t pa and pai 1 correlate with the formation of experimental post surgical peritoneal adhesions
url http://dx.doi.org/10.1155/MI/2006/13901
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