Study on histogenesis of enterochromaffin-like carcinoid in autoimmune atrophic gastritis associated with pernicious anemia
Background/Aim. Autoimmune atrophic fundic gastritis induces the pernicious anemia (PA), as well as the changes in both epithelium and endocrine cells of gastric mucosa. The most important complications are: achlorhydria, hypergastrinemia, gastric cancer and enterochromaffin-like ( ECL) carcinoid. T...
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Ministry of Defence of the Republic of Serbia, University of Defence, Belgrade
2007-01-01
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| Series: | Vojnosanitetski Pregled |
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| Online Access: | http://www.doiserbia.nb.rs/img/doi/0042-8450/2007/0042-84500708543M.pdf |
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| author | Mačukanović-Golubović Lana Katić Vuka Rančić Gorana Milenović Mladen Marjanović Goran Golubović Zoran |
| author_facet | Mačukanović-Golubović Lana Katić Vuka Rančić Gorana Milenović Mladen Marjanović Goran Golubović Zoran |
| author_sort | Mačukanović-Golubović Lana |
| collection | DOAJ |
| description | Background/Aim. Autoimmune atrophic fundic gastritis induces the pernicious anemia (PA), as well as the changes in both epithelium and endocrine cells of gastric mucosa. The most important complications are: achlorhydria, hypergastrinemia, gastric cancer and enterochromaffin-like ( ECL) carcinoid. The aim of this study was to examine ECL carcinoid histogenesis in A-gastritis associated with PA. Methods. During the period from 2000−2006, 65 patients with PA and 30 patients of the control group were examined. Histopathological examination was done in endoscopical biopsies of gastric mucosa fixed in 10% formaldehyde. Paraffin sections were stained with classic hematoxylin-eosin (HE); histochemical AB-PAS (pH 2.5), cytochemical argyrophilic Servier-Munger′s and immunocytochemical PAP methods for G cell identification and chromogranin A antibodies - specific marker for neuroendocrine ECL cells. Both G and ECL cells were counted per 20 fields, of surface 0.0245312 mm2 by a field. Basal gastrin serum levels were also examined by using radioimmunoassay (RIA) method. The obtained results were statisticaly calculated by using Student΄s t test. Results. Marked antral G cell hyperplasia associated with corporal ECL hyperplasia was found. ECL cell hyperplasia was of simplex, linear, adenomatoid type to the pattern of intramucous ECL cell carcinoid. An average number of G cells was statistically significant in the patients with PA as compared to the control group (p < 0.05) as well as an average number of ECL cells. Conclusion. We concluded that antral G cell hyperplasia accompanied by gastrinemia induces ECL hyperplasia and ECL corporal carcinoid in A-gastritis and that their histogenesis develops trough simple, linear and adenomatoide hyperplasia. . |
| format | Article |
| id | doaj-art-1204a8f65714471db53d2e9ec83ff8a0 |
| institution | OA Journals |
| issn | 0042-8450 |
| language | English |
| publishDate | 2007-01-01 |
| publisher | Ministry of Defence of the Republic of Serbia, University of Defence, Belgrade |
| record_format | Article |
| series | Vojnosanitetski Pregled |
| spelling | doaj-art-1204a8f65714471db53d2e9ec83ff8a02025-08-20T01:59:31ZengMinistry of Defence of the Republic of Serbia, University of Defence, BelgradeVojnosanitetski Pregled0042-84502007-01-0164854354810.2298/VSP0708543MStudy on histogenesis of enterochromaffin-like carcinoid in autoimmune atrophic gastritis associated with pernicious anemiaMačukanović-Golubović LanaKatić VukaRančić GoranaMilenović MladenMarjanović GoranGolubović ZoranBackground/Aim. Autoimmune atrophic fundic gastritis induces the pernicious anemia (PA), as well as the changes in both epithelium and endocrine cells of gastric mucosa. The most important complications are: achlorhydria, hypergastrinemia, gastric cancer and enterochromaffin-like ( ECL) carcinoid. The aim of this study was to examine ECL carcinoid histogenesis in A-gastritis associated with PA. Methods. During the period from 2000−2006, 65 patients with PA and 30 patients of the control group were examined. Histopathological examination was done in endoscopical biopsies of gastric mucosa fixed in 10% formaldehyde. Paraffin sections were stained with classic hematoxylin-eosin (HE); histochemical AB-PAS (pH 2.5), cytochemical argyrophilic Servier-Munger′s and immunocytochemical PAP methods for G cell identification and chromogranin A antibodies - specific marker for neuroendocrine ECL cells. Both G and ECL cells were counted per 20 fields, of surface 0.0245312 mm2 by a field. Basal gastrin serum levels were also examined by using radioimmunoassay (RIA) method. The obtained results were statisticaly calculated by using Student΄s t test. Results. Marked antral G cell hyperplasia associated with corporal ECL hyperplasia was found. ECL cell hyperplasia was of simplex, linear, adenomatoid type to the pattern of intramucous ECL cell carcinoid. An average number of G cells was statistically significant in the patients with PA as compared to the control group (p < 0.05) as well as an average number of ECL cells. Conclusion. We concluded that antral G cell hyperplasia accompanied by gastrinemia induces ECL hyperplasia and ECL corporal carcinoid in A-gastritis and that their histogenesis develops trough simple, linear and adenomatoide hyperplasia. .http://www.doiserbia.nb.rs/img/doi/0042-8450/2007/0042-84500708543M.pdfgastritis, atrophicanemia, perniciousautoimmune diseasescarcinoid tumorenterochromaffin cellshistological techniques |
| spellingShingle | Mačukanović-Golubović Lana Katić Vuka Rančić Gorana Milenović Mladen Marjanović Goran Golubović Zoran Study on histogenesis of enterochromaffin-like carcinoid in autoimmune atrophic gastritis associated with pernicious anemia Vojnosanitetski Pregled gastritis, atrophic anemia, pernicious autoimmune diseases carcinoid tumor enterochromaffin cells histological techniques |
| title | Study on histogenesis of enterochromaffin-like carcinoid in autoimmune atrophic gastritis associated with pernicious anemia |
| title_full | Study on histogenesis of enterochromaffin-like carcinoid in autoimmune atrophic gastritis associated with pernicious anemia |
| title_fullStr | Study on histogenesis of enterochromaffin-like carcinoid in autoimmune atrophic gastritis associated with pernicious anemia |
| title_full_unstemmed | Study on histogenesis of enterochromaffin-like carcinoid in autoimmune atrophic gastritis associated with pernicious anemia |
| title_short | Study on histogenesis of enterochromaffin-like carcinoid in autoimmune atrophic gastritis associated with pernicious anemia |
| title_sort | study on histogenesis of enterochromaffin like carcinoid in autoimmune atrophic gastritis associated with pernicious anemia |
| topic | gastritis, atrophic anemia, pernicious autoimmune diseases carcinoid tumor enterochromaffin cells histological techniques |
| url | http://www.doiserbia.nb.rs/img/doi/0042-8450/2007/0042-84500708543M.pdf |
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