Isolation, purification, and characterization of a lectin from Cassia senna seeds with analgesic and gastroprotective effects
Cassia senna, famous for its medicinal qualities, has been crucial in traditional medicine, providing remedies for many ailments. Its seeds, which are abundant in bioactive compounds such as lectins, have significant pharmaceutical potential. In this study, we investigated the biochemical intricacie...
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Elsevier
2025-05-01
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| Series: | Phytomedicine Plus |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2667031325000417 |
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| author | Maha B. Dafalla Sara A.A. Elmubarak Eva H. Naser Ahmed H. Idries Yusria E. Abdelrahim Entsar A. Abdalrhman Bashir M. Ahmed Makarim Elfadil M. Osman Amna K.E. Awadalla Reem M.A. Ebrahim Ashraf O. Abdellatif Ghada H. Haj Ali Atif A. Elagib Emadeldin H.E. Konozy |
| author_facet | Maha B. Dafalla Sara A.A. Elmubarak Eva H. Naser Ahmed H. Idries Yusria E. Abdelrahim Entsar A. Abdalrhman Bashir M. Ahmed Makarim Elfadil M. Osman Amna K.E. Awadalla Reem M.A. Ebrahim Ashraf O. Abdellatif Ghada H. Haj Ali Atif A. Elagib Emadeldin H.E. Konozy |
| author_sort | Maha B. Dafalla |
| collection | DOAJ |
| description | Cassia senna, famous for its medicinal qualities, has been crucial in traditional medicine, providing remedies for many ailments. Its seeds, which are abundant in bioactive compounds such as lectins, have significant pharmaceutical potential. In this study, we investigated the biochemical intricacies and therapeutic potential of CsSL, a lectin found in Cassia senna seeds. Hemagglutination assays revealed robust CsSL activity, reaching its peak with 50 mM Tris–HCl buffer at pH 7.5. Ammonium sulfate fractionation was used to purify CsSL, with Fr40 showing the highest activity, confirming successful isolation. GlcNAc-agarose affinity chromatography further purified CsSL, showing its affinity for galactose residues. CsSL is stable, retains its full activity for 45 min at 30–40 °C, and exhibits pH dependence, with peak hemagglutinating activity occurring between pH 3.5 and 4.5 and sharply declining beyond pH 7. N-bromosuccinimide (NBS) modification showed that half of CsSL's tryptophan residues is crucial for its activity, as evidenced by decreased lectin activity. Therapeutically, CsSL demonstrated notable analgesic effects in acetic acid-induced writhing and thermal sensitivity tests. The 12 mg/kg dose particularly showed a remarkable antinociceptive effect (p < 0.05), completely reducing the writhing reflex by 100 % compared to that of the negative control Moreover, CsSL had potent gastroprotective effects, reducing ethanol-induced gastric lesions in a dose-dependent manner. Notably, doses of 0.25 mg/kg and 0.5 mg/kg provided substantial stomach lining protection (p < 0.05), outperforming the standard ulcer medication pantoprazole. CsSL exhibits promise for pain relief and ulcer prevention, with significant analgesic and gastroprotective effects. However, further research is needed to fully understand its underlying mechanisms and clinical applications, as this research has exciting potential in biomedicine and biotechnology. |
| format | Article |
| id | doaj-art-12037ea28f13421bbc0cddcb7b6068a9 |
| institution | Kabale University |
| issn | 2667-0313 |
| language | English |
| publishDate | 2025-05-01 |
| publisher | Elsevier |
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| spelling | doaj-art-12037ea28f13421bbc0cddcb7b6068a92025-08-20T03:53:13ZengElsevierPhytomedicine Plus2667-03132025-05-015210076810.1016/j.phyplu.2025.100768Isolation, purification, and characterization of a lectin from Cassia senna seeds with analgesic and gastroprotective effectsMaha B. Dafalla0Sara A.A. Elmubarak1Eva H. Naser2Ahmed H. Idries3Yusria E. Abdelrahim4Entsar A. Abdalrhman5Bashir M. Ahmed6Makarim Elfadil M. Osman7Amna K.E. Awadalla8Reem M.A. Ebrahim9Ashraf O. Abdellatif10Ghada H. Haj Ali11Atif A. Elagib12Emadeldin H.E. Konozy13Biotechnology Department, Africa City of Technology, Khartoum, SudanBiotechnology Department, Africa City of Technology, Khartoum, SudanBiotechnology Department, Africa City of Technology, Khartoum, SudanBiotechnology Department, Africa City of Technology, Khartoum, SudanBiotechnology Department, Africa City of Technology, Khartoum, SudanBiotechnology Department, Africa City of Technology, Khartoum, SudanMedicinal, Aromatic Plants and Traditional Medicine Research Institute (MAPTRI), National Center for Research, Khartoum, SudanBiotechnology Department, Africa City of Technology, Khartoum, SudanBiotechnology Department, Africa City of Technology, Khartoum, SudanBiotechnology Department, Africa City of Technology, Khartoum, SudanPharmaceutical Research and Development Centre, Faculty of Pharmacy, Karary University, Omdurman, SudanFaculty of Science and Technology, Al Neelain University, Khartoum, SudanThe National Centre for Research, Khartoum, SudanBiotechnology Department, Africa City of Technology, Khartoum, Sudan; Biomedical and Clinical Research Centre (BCRC), College of Health and Allied Sciences (CoHAS), University of Cape Coast, Cape Coast, Ghana; Corresponding author at: Biomedical and Clinical Research Centre (BCRC), College of Health and Allied Sciences (CoHAS), University of Cape Coast, Cape Coast, Ghana.Cassia senna, famous for its medicinal qualities, has been crucial in traditional medicine, providing remedies for many ailments. Its seeds, which are abundant in bioactive compounds such as lectins, have significant pharmaceutical potential. In this study, we investigated the biochemical intricacies and therapeutic potential of CsSL, a lectin found in Cassia senna seeds. Hemagglutination assays revealed robust CsSL activity, reaching its peak with 50 mM Tris–HCl buffer at pH 7.5. Ammonium sulfate fractionation was used to purify CsSL, with Fr40 showing the highest activity, confirming successful isolation. GlcNAc-agarose affinity chromatography further purified CsSL, showing its affinity for galactose residues. CsSL is stable, retains its full activity for 45 min at 30–40 °C, and exhibits pH dependence, with peak hemagglutinating activity occurring between pH 3.5 and 4.5 and sharply declining beyond pH 7. N-bromosuccinimide (NBS) modification showed that half of CsSL's tryptophan residues is crucial for its activity, as evidenced by decreased lectin activity. Therapeutically, CsSL demonstrated notable analgesic effects in acetic acid-induced writhing and thermal sensitivity tests. The 12 mg/kg dose particularly showed a remarkable antinociceptive effect (p < 0.05), completely reducing the writhing reflex by 100 % compared to that of the negative control Moreover, CsSL had potent gastroprotective effects, reducing ethanol-induced gastric lesions in a dose-dependent manner. Notably, doses of 0.25 mg/kg and 0.5 mg/kg provided substantial stomach lining protection (p < 0.05), outperforming the standard ulcer medication pantoprazole. CsSL exhibits promise for pain relief and ulcer prevention, with significant analgesic and gastroprotective effects. However, further research is needed to fully understand its underlying mechanisms and clinical applications, as this research has exciting potential in biomedicine and biotechnology.http://www.sciencedirect.com/science/article/pii/S2667031325000417LectinsMedicinal plantCassia sennaPurificationCharacterizationBiological activities |
| spellingShingle | Maha B. Dafalla Sara A.A. Elmubarak Eva H. Naser Ahmed H. Idries Yusria E. Abdelrahim Entsar A. Abdalrhman Bashir M. Ahmed Makarim Elfadil M. Osman Amna K.E. Awadalla Reem M.A. Ebrahim Ashraf O. Abdellatif Ghada H. Haj Ali Atif A. Elagib Emadeldin H.E. Konozy Isolation, purification, and characterization of a lectin from Cassia senna seeds with analgesic and gastroprotective effects Phytomedicine Plus Lectins Medicinal plant Cassia senna Purification Characterization Biological activities |
| title | Isolation, purification, and characterization of a lectin from Cassia senna seeds with analgesic and gastroprotective effects |
| title_full | Isolation, purification, and characterization of a lectin from Cassia senna seeds with analgesic and gastroprotective effects |
| title_fullStr | Isolation, purification, and characterization of a lectin from Cassia senna seeds with analgesic and gastroprotective effects |
| title_full_unstemmed | Isolation, purification, and characterization of a lectin from Cassia senna seeds with analgesic and gastroprotective effects |
| title_short | Isolation, purification, and characterization of a lectin from Cassia senna seeds with analgesic and gastroprotective effects |
| title_sort | isolation purification and characterization of a lectin from cassia senna seeds with analgesic and gastroprotective effects |
| topic | Lectins Medicinal plant Cassia senna Purification Characterization Biological activities |
| url | http://www.sciencedirect.com/science/article/pii/S2667031325000417 |
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