Genomic, transcriptomic and metabolomic analyses of Amorphophallus albus provides insights into the evolution and resistance to southern blight pathogen
IntroductionAmorphophallus albus, a perennial herb in the Araceae family, is a valuable cash crop known for its high production of konjac glucomannan and high disease resistance.MethodsIn this study, we present a high-quality, chromosome-scale genome assembly of A. albus using a combination of PacBi...
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Frontiers Media S.A.
2025-02-01
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| author | Longfei Duan Longfei Duan Jianfeng Qin Gaoxin Zhou Chuan Shen Baofu Qin |
| author_facet | Longfei Duan Longfei Duan Jianfeng Qin Gaoxin Zhou Chuan Shen Baofu Qin |
| author_sort | Longfei Duan |
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| description | IntroductionAmorphophallus albus, a perennial herb in the Araceae family, is a valuable cash crop known for its high production of konjac glucomannan and high disease resistance.MethodsIn this study, we present a high-quality, chromosome-scale genome assembly of A. albus using a combination of PacBio HiFi sequencing, DNBSEQ short-read sequencing, and Hi-C technology. To elucidate the molecular mechanisms underlying southern blight resistance, we performed an integrated analysis of transcriptomic and metabolomic profiles across three infection stages of A. albus.Results and discussionHere, we assembled and annotated the complete genome of A. albus, providing a chromosome-level assembly with a total genome size of 5.94 Gb and a contig N50 of 5.61 Mb. The A. albus genome comprised 19,908 gene families, including 467 unique families.The slightly larger genome size of A. albus compared to A. konjac may have been affected by a recent whole-genome duplication event. Transcriptional and metabolic analyses revealed significant enrichment of differentially expressed genes (DEGs) and differentially accumulated metabolites (DAMs) involved in phenylpropanoid biosynthesis, plant hormone signal transduction, phenylalanine metabolism, and the biosynthesis of phenylalanine, tyrosine, and tryptophan. These findings not only advance the understanding of genetic and evolutionary characteristics of A. albus but also provide a foundation for future research on the resistance mechanisms of konjac against southern blight disease. |
| format | Article |
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| institution | Kabale University |
| issn | 1664-462X |
| language | English |
| publishDate | 2025-02-01 |
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| spelling | doaj-art-120278c9fe4547dd834fb03f49fb09772025-02-07T06:49:58ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2025-02-011510.3389/fpls.2024.15180581518058Genomic, transcriptomic and metabolomic analyses of Amorphophallus albus provides insights into the evolution and resistance to southern blight pathogenLongfei Duan0Longfei Duan1Jianfeng Qin2Gaoxin Zhou3Chuan Shen4Baofu Qin5Laboratory of Life Sciences, College of Life Sciences, Northwest A&F University, Xianyang, Shaanxi, ChinaAnkang Academy of Agricultural Sciences, Ankang, Shaanxi, ChinaAnkang Academy of Agricultural Sciences, Ankang, Shaanxi, ChinaAnkang Academy of Agricultural Sciences, Ankang, Shaanxi, ChinaCollege of Economics and Management, Ankang University, Ankang, Shaanxi, ChinaLaboratory of Life Sciences, College of Life Sciences, Northwest A&F University, Xianyang, Shaanxi, ChinaIntroductionAmorphophallus albus, a perennial herb in the Araceae family, is a valuable cash crop known for its high production of konjac glucomannan and high disease resistance.MethodsIn this study, we present a high-quality, chromosome-scale genome assembly of A. albus using a combination of PacBio HiFi sequencing, DNBSEQ short-read sequencing, and Hi-C technology. To elucidate the molecular mechanisms underlying southern blight resistance, we performed an integrated analysis of transcriptomic and metabolomic profiles across three infection stages of A. albus.Results and discussionHere, we assembled and annotated the complete genome of A. albus, providing a chromosome-level assembly with a total genome size of 5.94 Gb and a contig N50 of 5.61 Mb. The A. albus genome comprised 19,908 gene families, including 467 unique families.The slightly larger genome size of A. albus compared to A. konjac may have been affected by a recent whole-genome duplication event. Transcriptional and metabolic analyses revealed significant enrichment of differentially expressed genes (DEGs) and differentially accumulated metabolites (DAMs) involved in phenylpropanoid biosynthesis, plant hormone signal transduction, phenylalanine metabolism, and the biosynthesis of phenylalanine, tyrosine, and tryptophan. These findings not only advance the understanding of genetic and evolutionary characteristics of A. albus but also provide a foundation for future research on the resistance mechanisms of konjac against southern blight disease.https://www.frontiersin.org/articles/10.3389/fpls.2024.1518058/fullAmorphophallus albusgenome evolutiontranscriptomic and metabolomicwhole genome duplicationSclerotium rolfsii (SR) |
| spellingShingle | Longfei Duan Longfei Duan Jianfeng Qin Gaoxin Zhou Chuan Shen Baofu Qin Genomic, transcriptomic and metabolomic analyses of Amorphophallus albus provides insights into the evolution and resistance to southern blight pathogen Frontiers in Plant Science Amorphophallus albus genome evolution transcriptomic and metabolomic whole genome duplication Sclerotium rolfsii (SR) |
| title | Genomic, transcriptomic and metabolomic analyses of Amorphophallus albus provides insights into the evolution and resistance to southern blight pathogen |
| title_full | Genomic, transcriptomic and metabolomic analyses of Amorphophallus albus provides insights into the evolution and resistance to southern blight pathogen |
| title_fullStr | Genomic, transcriptomic and metabolomic analyses of Amorphophallus albus provides insights into the evolution and resistance to southern blight pathogen |
| title_full_unstemmed | Genomic, transcriptomic and metabolomic analyses of Amorphophallus albus provides insights into the evolution and resistance to southern blight pathogen |
| title_short | Genomic, transcriptomic and metabolomic analyses of Amorphophallus albus provides insights into the evolution and resistance to southern blight pathogen |
| title_sort | genomic transcriptomic and metabolomic analyses of amorphophallus albus provides insights into the evolution and resistance to southern blight pathogen |
| topic | Amorphophallus albus genome evolution transcriptomic and metabolomic whole genome duplication Sclerotium rolfsii (SR) |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2024.1518058/full |
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