Emergence of Flucytosine-Resistant Candida tropicalis Clade, the Netherlands
Candida tropicalis is the second most virulent Candida species after C. albicans. Previous studies from the Netherlands and France reported a notable reduction in susceptibility to flucytosine (5-FC) in a substantial proportion of C. tropicalis isolates. We investigated epidemiologic patterns of C....
Saved in:
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Centers for Disease Control and Prevention
2025-07-01
|
| Series: | Emerging Infectious Diseases |
| Subjects: | |
| Online Access: | https://wwwnc.cdc.gov/eid/article/31/7/24-1918_article |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849425507065528320 |
|---|---|
| author | Fatima Zohra Delma Bram Spruijtenburg Jacques F. Meis Auke W. de Jong James Groot Johanna Rhodes Willem J.G. Melchers Paul E. Verweij Theun de Groot Eelco F.J. Meijer Jochem B. Buil |
| author_facet | Fatima Zohra Delma Bram Spruijtenburg Jacques F. Meis Auke W. de Jong James Groot Johanna Rhodes Willem J.G. Melchers Paul E. Verweij Theun de Groot Eelco F.J. Meijer Jochem B. Buil |
| author_sort | Fatima Zohra Delma |
| collection | DOAJ |
| description |
Candida tropicalis is the second most virulent Candida species after C. albicans. Previous studies from the Netherlands and France reported a notable reduction in susceptibility to flucytosine (5-FC) in a substantial proportion of C. tropicalis isolates. We investigated epidemiologic patterns of C. tropicalis isolates in the Netherlands and the genetic mechanisms driving widespread non–wild-type (WT) 5-FC resistance. We conducted antifungal susceptibility testing and used advanced molecular techniques, including short tandem repeat genotyping and whole-genome sequencing paired with single-nucleotide polymorphism analysis, to analyze 250 C. tropicalis isolates collected across the Netherlands during 2012–2022. Our findings revealed the rapid emergence of a 5-FC–resistant, non-WT C. tropicalis clade, accounting for >40% of all C. tropicalis isolates by 2022. Genomic analysis identified a homozygous nonsense mutation in the FCY2 gene, which was exclusive to this non-WT population. Continued surveillance efforts are needed to detect and prevent the spread of drug-resistant Candida species.
|
| format | Article |
| id | doaj-art-11fab2e97c54437bb36cf2bd389e0c85 |
| institution | Kabale University |
| issn | 1080-6040 1080-6059 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | Centers for Disease Control and Prevention |
| record_format | Article |
| series | Emerging Infectious Diseases |
| spelling | doaj-art-11fab2e97c54437bb36cf2bd389e0c852025-08-20T03:29:44ZengCenters for Disease Control and PreventionEmerging Infectious Diseases1080-60401080-60592025-07-013171354136410.3201/eid3107.241918Emergence of Flucytosine-Resistant Candida tropicalis Clade, the NetherlandsFatima Zohra DelmaBram SpruijtenburgJacques F. MeisAuke W. de JongJames GrootJohanna RhodesWillem J.G. MelchersPaul E. VerweijTheun de GrootEelco F.J. MeijerJochem B. Buil Candida tropicalis is the second most virulent Candida species after C. albicans. Previous studies from the Netherlands and France reported a notable reduction in susceptibility to flucytosine (5-FC) in a substantial proportion of C. tropicalis isolates. We investigated epidemiologic patterns of C. tropicalis isolates in the Netherlands and the genetic mechanisms driving widespread non–wild-type (WT) 5-FC resistance. We conducted antifungal susceptibility testing and used advanced molecular techniques, including short tandem repeat genotyping and whole-genome sequencing paired with single-nucleotide polymorphism analysis, to analyze 250 C. tropicalis isolates collected across the Netherlands during 2012–2022. Our findings revealed the rapid emergence of a 5-FC–resistant, non-WT C. tropicalis clade, accounting for >40% of all C. tropicalis isolates by 2022. Genomic analysis identified a homozygous nonsense mutation in the FCY2 gene, which was exclusive to this non-WT population. Continued surveillance efforts are needed to detect and prevent the spread of drug-resistant Candida species. https://wwwnc.cdc.gov/eid/article/31/7/24-1918_articleCandidaCandida tropicalisflucytosineantifungal resistancegenotypingwhole-genome sequencing |
| spellingShingle | Fatima Zohra Delma Bram Spruijtenburg Jacques F. Meis Auke W. de Jong James Groot Johanna Rhodes Willem J.G. Melchers Paul E. Verweij Theun de Groot Eelco F.J. Meijer Jochem B. Buil Emergence of Flucytosine-Resistant Candida tropicalis Clade, the Netherlands Emerging Infectious Diseases Candida Candida tropicalis flucytosine antifungal resistance genotyping whole-genome sequencing |
| title | Emergence of Flucytosine-Resistant Candida tropicalis Clade, the Netherlands |
| title_full | Emergence of Flucytosine-Resistant Candida tropicalis Clade, the Netherlands |
| title_fullStr | Emergence of Flucytosine-Resistant Candida tropicalis Clade, the Netherlands |
| title_full_unstemmed | Emergence of Flucytosine-Resistant Candida tropicalis Clade, the Netherlands |
| title_short | Emergence of Flucytosine-Resistant Candida tropicalis Clade, the Netherlands |
| title_sort | emergence of flucytosine resistant candida tropicalis clade the netherlands |
| topic | Candida Candida tropicalis flucytosine antifungal resistance genotyping whole-genome sequencing |
| url | https://wwwnc.cdc.gov/eid/article/31/7/24-1918_article |
| work_keys_str_mv | AT fatimazohradelma emergenceofflucytosineresistantcandidatropicaliscladethenetherlands AT bramspruijtenburg emergenceofflucytosineresistantcandidatropicaliscladethenetherlands AT jacquesfmeis emergenceofflucytosineresistantcandidatropicaliscladethenetherlands AT aukewdejong emergenceofflucytosineresistantcandidatropicaliscladethenetherlands AT jamesgroot emergenceofflucytosineresistantcandidatropicaliscladethenetherlands AT johannarhodes emergenceofflucytosineresistantcandidatropicaliscladethenetherlands AT willemjgmelchers emergenceofflucytosineresistantcandidatropicaliscladethenetherlands AT pauleverweij emergenceofflucytosineresistantcandidatropicaliscladethenetherlands AT theundegroot emergenceofflucytosineresistantcandidatropicaliscladethenetherlands AT eelcofjmeijer emergenceofflucytosineresistantcandidatropicaliscladethenetherlands AT jochembbuil emergenceofflucytosineresistantcandidatropicaliscladethenetherlands |