Decoction of Yougui Wan combined with Wuzi Yanzong Wan ameliorates thin endometrium in a mouse model
Background This study aimed to determine the effect and mechanism of the Decoction of Yougui Wan combined with Wuzi Yanzong Wan (DYWWYW), a traditional Chinese herbal formula, in a mouse model with thin endometrium induced by 95% ethanol.Methods Thin endometrium mice were treated with progynova (0.0...
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Taylor & Francis Group
2024-12-01
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| Series: | Journal of Obstetrics and Gynaecology |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/01443615.2024.2391062 |
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| _version_ | 1841553128364703744 |
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| author | Xiaohong Zhou Jun Xiang Aifang Su |
| author_facet | Xiaohong Zhou Jun Xiang Aifang Su |
| author_sort | Xiaohong Zhou |
| collection | DOAJ |
| description | Background This study aimed to determine the effect and mechanism of the Decoction of Yougui Wan combined with Wuzi Yanzong Wan (DYWWYW), a traditional Chinese herbal formula, in a mouse model with thin endometrium induced by 95% ethanol.Methods Thin endometrium mice were treated with progynova (0.002 mg) as well as a low and high dose of DYWWYW (0.05 and 0.5 mL DYWWYW, respectively, diluted in 2 mL normal saline). Western blotting and qRT-PCR analyses were performed to determine the protein and mRNA expression levels, respectively, of integrin αγβ3 and leukaemia inhibitor factor (LIF) in uterus tissues. Serum oestradiol and progesterone concentrations were determined via ELISA. The remaining thin endometrium mice were mated with male mice, and the number of embryos implanted in the different groups was calculated.Results A high dose of DYWWYW effectively ameliorated the injury of endometrium caused by 95% ethanol. The levels of oestradiol, progesterone, αγβ3 and LIF in thin endometrium mice treated with a high dose of DYWWYW were also significantly elevated. Additionally, a high dose of DYWWYW remarkably increased the number of embryo implantations in mice with thin endometrium.Conclusion DYWWYW has improvement effects on thin endometrium by elevating the levels of endogenous oestradiol, progesterone, αγβ3, and LIF in a mouse model. |
| format | Article |
| id | doaj-art-11f0644c5fb04498a961dff866887541 |
| institution | Kabale University |
| issn | 0144-3615 1364-6893 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Journal of Obstetrics and Gynaecology |
| spelling | doaj-art-11f0644c5fb04498a961dff8668875412025-01-09T12:13:17ZengTaylor & Francis GroupJournal of Obstetrics and Gynaecology0144-36151364-68932024-12-0144110.1080/01443615.2024.2391062Decoction of Yougui Wan combined with Wuzi Yanzong Wan ameliorates thin endometrium in a mouse modelXiaohong Zhou0Jun Xiang1Aifang Su2Department of Gynecology, Jinhua Maternal & Child Health Care Hospital, Jinhua, Zhejiang, ChinaDepartment of Gynecology, Jinhua Maternal & Child Health Care Hospital, Jinhua, Zhejiang, ChinaDepartment of Gynecology, Jinhua Maternal & Child Health Care Hospital, Jinhua, Zhejiang, ChinaBackground This study aimed to determine the effect and mechanism of the Decoction of Yougui Wan combined with Wuzi Yanzong Wan (DYWWYW), a traditional Chinese herbal formula, in a mouse model with thin endometrium induced by 95% ethanol.Methods Thin endometrium mice were treated with progynova (0.002 mg) as well as a low and high dose of DYWWYW (0.05 and 0.5 mL DYWWYW, respectively, diluted in 2 mL normal saline). Western blotting and qRT-PCR analyses were performed to determine the protein and mRNA expression levels, respectively, of integrin αγβ3 and leukaemia inhibitor factor (LIF) in uterus tissues. Serum oestradiol and progesterone concentrations were determined via ELISA. The remaining thin endometrium mice were mated with male mice, and the number of embryos implanted in the different groups was calculated.Results A high dose of DYWWYW effectively ameliorated the injury of endometrium caused by 95% ethanol. The levels of oestradiol, progesterone, αγβ3 and LIF in thin endometrium mice treated with a high dose of DYWWYW were also significantly elevated. Additionally, a high dose of DYWWYW remarkably increased the number of embryo implantations in mice with thin endometrium.Conclusion DYWWYW has improvement effects on thin endometrium by elevating the levels of endogenous oestradiol, progesterone, αγβ3, and LIF in a mouse model.https://www.tandfonline.com/doi/10.1080/01443615.2024.2391062Yougui WanWuzi Yanzong Wanthin endometriumαγβ3leukaemia inhibitor factorhormone |
| spellingShingle | Xiaohong Zhou Jun Xiang Aifang Su Decoction of Yougui Wan combined with Wuzi Yanzong Wan ameliorates thin endometrium in a mouse model Journal of Obstetrics and Gynaecology Yougui Wan Wuzi Yanzong Wan thin endometrium αγβ3 leukaemia inhibitor factor hormone |
| title | Decoction of Yougui Wan combined with Wuzi Yanzong Wan ameliorates thin endometrium in a mouse model |
| title_full | Decoction of Yougui Wan combined with Wuzi Yanzong Wan ameliorates thin endometrium in a mouse model |
| title_fullStr | Decoction of Yougui Wan combined with Wuzi Yanzong Wan ameliorates thin endometrium in a mouse model |
| title_full_unstemmed | Decoction of Yougui Wan combined with Wuzi Yanzong Wan ameliorates thin endometrium in a mouse model |
| title_short | Decoction of Yougui Wan combined with Wuzi Yanzong Wan ameliorates thin endometrium in a mouse model |
| title_sort | decoction of yougui wan combined with wuzi yanzong wan ameliorates thin endometrium in a mouse model |
| topic | Yougui Wan Wuzi Yanzong Wan thin endometrium αγβ3 leukaemia inhibitor factor hormone |
| url | https://www.tandfonline.com/doi/10.1080/01443615.2024.2391062 |
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