Application of a degradable thin film to modulate perfusion to post-autotransplantation airways in ratsCentral MessagePerspective
Objective: Recipients of lung transplants experience the lowest long-term survival among all solid-organ transplant recipients. Airway complications contribute significantly to morbidity and mortality post-lung transplant and may be driven by airway devascularization inherent to procurement and impl...
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Elsevier
2025-04-01
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666273625000348 |
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| author | Aravind Krishnan, MD Mahdi Forouharshad, PhD Elbert Heng, MD Alyssa Garrison, MS Daniel Alnasir, BSE Shubham Patil, BS Arman Farazdaghi, BS Moeed Fawad, MS Stefan Elde, MD Brandon A. Guenthart, MD Laura M. Ensign, PhD Y Joseph Woo, MD Kunal S. Parikh, PhD John W. MacArthur, MD |
| author_facet | Aravind Krishnan, MD Mahdi Forouharshad, PhD Elbert Heng, MD Alyssa Garrison, MS Daniel Alnasir, BSE Shubham Patil, BS Arman Farazdaghi, BS Moeed Fawad, MS Stefan Elde, MD Brandon A. Guenthart, MD Laura M. Ensign, PhD Y Joseph Woo, MD Kunal S. Parikh, PhD John W. MacArthur, MD |
| author_sort | Aravind Krishnan, MD |
| collection | DOAJ |
| description | Objective: Recipients of lung transplants experience the lowest long-term survival among all solid-organ transplant recipients. Airway complications contribute significantly to morbidity and mortality post-lung transplant and may be driven by airway devascularization inherent to procurement and implantation of the lungs. We studied application of biodegradable, nanofiber-based thin films to devascularized autotransplanted airways to mitigate airway ischemia. Methods: We used a rat tracheal autotransplantation model that replicates airway ischemia. Rats were divided into an operated control group (n = 18) and a treatment group (n = 12) receiving an electrospun film composed of randomly aligned polydioxanone (PDO) nanofibers applied to the circumferential surface of the transplanted trachea. Airway perfusion was assessed via laser speckle contrast analysis at 0, 3, and 10 days. Differences in perfusion units were calculated between the nontransplanted and transplanted segments of the trachea. Multimodal analysis of angiogenesis in tracheal autografts included immunoassay profiling for proangiogenic cytokines, histologic injury grading, and speckle angiography. Results: Qualitative and quantitative perfusion differences were demonstrated at days 0, 3, and 10. Nanofiber-based, PDO thin films significantly improved perfusion in the transplanted segment of trachea (P < .05). Histologic injury scoring was significantly worse in the operated controls compared with the treatment group (P < .01). Immunoassays demonstrated increased expression of vascular cell adhesion molecule 1 in the treatment group (P < .05). Conclusions: Application of a nanofiber-based, PDO thin film induced a local tissue response that improved perfusion and histologic injury scoring of the transplanted airway in an autotransplant model of airway devascularization. Immune multiplexing suggests local inflammatory responses may drive angiogenesis. |
| format | Article |
| id | doaj-art-11ef5cd3ead946beadf619749d9df17a |
| institution | OA Journals |
| issn | 2666-2736 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | Elsevier |
| record_format | Article |
| series | JTCVS Open |
| spelling | doaj-art-11ef5cd3ead946beadf619749d9df17a2025-08-20T02:12:08ZengElsevierJTCVS Open2666-27362025-04-012451052010.1016/j.xjon.2025.01.008Application of a degradable thin film to modulate perfusion to post-autotransplantation airways in ratsCentral MessagePerspectiveAravind Krishnan, MD0Mahdi Forouharshad, PhD1Elbert Heng, MD2Alyssa Garrison, MS3Daniel Alnasir, BSE4Shubham Patil, BS5Arman Farazdaghi, BS6Moeed Fawad, MS7Stefan Elde, MD8Brandon A. Guenthart, MD9Laura M. Ensign, PhD10Y Joseph Woo, MD11Kunal S. Parikh, PhD12John W. MacArthur, MD13Department of Cardiothoracic Surgery, Stanford University School of Medicine, Stanford, CalifCenter for Nanomedicine at the Wilmer Eye Institute, Department of Ophthalmology, Johns Hopkins University School of Medicine, Baltimore, MdDepartment of Cardiothoracic Surgery, Stanford University School of Medicine, Stanford, CalifDepartment of Cardiothoracic Surgery, Stanford University School of Medicine, Stanford, CalifDepartment of Cardiothoracic Surgery, Stanford University School of Medicine, Stanford, CalifDepartment of Chemical and Biomolecular Engineering, Johns Hopkins University, Baltimore, MdDepartment of Chemical and Biomolecular Engineering, Johns Hopkins University, Baltimore, MdDepartment of Cardiothoracic Surgery, Stanford University School of Medicine, Stanford, CalifDepartment of Cardiothoracic Surgery, Stanford University School of Medicine, Stanford, CalifDepartment of Cardiothoracic Surgery, Stanford University School of Medicine, Stanford, CalifCenter for Nanomedicine at the Wilmer Eye Institute, Department of Ophthalmology, Johns Hopkins University School of Medicine, Baltimore, Md; Department of Chemical and Biomolecular Engineering, Johns Hopkins University, Baltimore, MdDepartment of Cardiothoracic Surgery, Stanford University School of Medicine, Stanford, CalifCenter for Nanomedicine at the Wilmer Eye Institute, Department of Ophthalmology, Johns Hopkins University School of Medicine, Baltimore, Md; Glaucoma Center or Excellence, Department of Ophthalmology, Johns Hopkins University School of Medicine, Baltimore, Md; Center for Bioengineering Innovation & Design, Department of Biomedical Engineering, Johns Hopkins University School of Medicine, Baltimore, Md; Kunal S. Parikh, PhD, Department of Ophthalmology, Johns Hopkins University School of Medicine, 6037 Smith Building, 400 N Broadway, Baltimore, MD 21231.Department of Cardiothoracic Surgery, Stanford University School of Medicine, Stanford, Calif; Address for reprints: John W. MacArthur, MD, Department of Cardiothoracic Surgery, Stanford University Medical Center, Falk Building CV-235, 300 Pasteur Dr, Stanford, CA 94305-5407.Objective: Recipients of lung transplants experience the lowest long-term survival among all solid-organ transplant recipients. Airway complications contribute significantly to morbidity and mortality post-lung transplant and may be driven by airway devascularization inherent to procurement and implantation of the lungs. We studied application of biodegradable, nanofiber-based thin films to devascularized autotransplanted airways to mitigate airway ischemia. Methods: We used a rat tracheal autotransplantation model that replicates airway ischemia. Rats were divided into an operated control group (n = 18) and a treatment group (n = 12) receiving an electrospun film composed of randomly aligned polydioxanone (PDO) nanofibers applied to the circumferential surface of the transplanted trachea. Airway perfusion was assessed via laser speckle contrast analysis at 0, 3, and 10 days. Differences in perfusion units were calculated between the nontransplanted and transplanted segments of the trachea. Multimodal analysis of angiogenesis in tracheal autografts included immunoassay profiling for proangiogenic cytokines, histologic injury grading, and speckle angiography. Results: Qualitative and quantitative perfusion differences were demonstrated at days 0, 3, and 10. Nanofiber-based, PDO thin films significantly improved perfusion in the transplanted segment of trachea (P < .05). Histologic injury scoring was significantly worse in the operated controls compared with the treatment group (P < .01). Immunoassays demonstrated increased expression of vascular cell adhesion molecule 1 in the treatment group (P < .05). Conclusions: Application of a nanofiber-based, PDO thin film induced a local tissue response that improved perfusion and histologic injury scoring of the transplanted airway in an autotransplant model of airway devascularization. Immune multiplexing suggests local inflammatory responses may drive angiogenesis.http://www.sciencedirect.com/science/article/pii/S2666273625000348airway ischemialung transplantangiogenesis |
| spellingShingle | Aravind Krishnan, MD Mahdi Forouharshad, PhD Elbert Heng, MD Alyssa Garrison, MS Daniel Alnasir, BSE Shubham Patil, BS Arman Farazdaghi, BS Moeed Fawad, MS Stefan Elde, MD Brandon A. Guenthart, MD Laura M. Ensign, PhD Y Joseph Woo, MD Kunal S. Parikh, PhD John W. MacArthur, MD Application of a degradable thin film to modulate perfusion to post-autotransplantation airways in ratsCentral MessagePerspective JTCVS Open airway ischemia lung transplant angiogenesis |
| title | Application of a degradable thin film to modulate perfusion to post-autotransplantation airways in ratsCentral MessagePerspective |
| title_full | Application of a degradable thin film to modulate perfusion to post-autotransplantation airways in ratsCentral MessagePerspective |
| title_fullStr | Application of a degradable thin film to modulate perfusion to post-autotransplantation airways in ratsCentral MessagePerspective |
| title_full_unstemmed | Application of a degradable thin film to modulate perfusion to post-autotransplantation airways in ratsCentral MessagePerspective |
| title_short | Application of a degradable thin film to modulate perfusion to post-autotransplantation airways in ratsCentral MessagePerspective |
| title_sort | application of a degradable thin film to modulate perfusion to post autotransplantation airways in ratscentral messageperspective |
| topic | airway ischemia lung transplant angiogenesis |
| url | http://www.sciencedirect.com/science/article/pii/S2666273625000348 |
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