Expression analysis of CsNAC30 gene in cucumber under drought stress
This study performed a bioinformatics analysis of the CsNAC30 gene and examined its expression levels in cucumber leaves and roots under drought stress induced by PEG-6000. The bioinformatics analysis showed that the open reading frame (ORF) of CsNAC30 is 1254 bp, encoding a protein consisting of 41...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2024-12-01
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| Series: | Biotechnology & Biotechnological Equipment |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/13102818.2024.2425692 |
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| author | Lei Gong Linna Qian Chengcheng Shen Yuping Wei Wenjiao Wang |
| author_facet | Lei Gong Linna Qian Chengcheng Shen Yuping Wei Wenjiao Wang |
| author_sort | Lei Gong |
| collection | DOAJ |
| description | This study performed a bioinformatics analysis of the CsNAC30 gene and examined its expression levels in cucumber leaves and roots under drought stress induced by PEG-6000. The bioinformatics analysis showed that the open reading frame (ORF) of CsNAC30 is 1254 bp, encoding a protein consisting of 417 amino acids. This protein is relatively unstable and hydrophilic, lacks transmembrane regions, and contains 49 phosphorylation sites. It also features a conserved NAM domain at the N-terminus. Homology and phylogenetic analyses indicate that CsNAC30 is a member of the NAC transcription factor family and is most closely related to the melon CMeNAC105 and the winter melon BhNAC96. Quantitative real-time PCR (qPCR) revealed that CsNAC30 expression is induced by drought stress, with relative levels increasing in both leaves and roots following PEG treatment. Expression peaked at 6 h in leaves and 9 h in roots before decreasing. These findings suggest that CsNAC30 plays a role in the cucumber’s response to drought stress. |
| format | Article |
| id | doaj-art-11ee567ca4dc4f8eb96c3d8d75d25574 |
| institution | OA Journals |
| issn | 1310-2818 1314-3530 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Biotechnology & Biotechnological Equipment |
| spelling | doaj-art-11ee567ca4dc4f8eb96c3d8d75d255742025-08-20T02:22:10ZengTaylor & Francis GroupBiotechnology & Biotechnological Equipment1310-28181314-35302024-12-0138110.1080/13102818.2024.2425692Expression analysis of CsNAC30 gene in cucumber under drought stressLei Gong0Linna Qian1Chengcheng Shen2Yuping Wei3Wenjiao Wang4College of Horticulture, Shanxi Agricultural University, Jinzhong, ChinaCollege of Horticulture, Shanxi Agricultural University, Jinzhong, ChinaCollege of Horticulture, Shanxi Agricultural University, Jinzhong, ChinaCollege of Horticulture, Shanxi Agricultural University, Jinzhong, ChinaCollege of Horticulture, Shanxi Agricultural University, Jinzhong, ChinaThis study performed a bioinformatics analysis of the CsNAC30 gene and examined its expression levels in cucumber leaves and roots under drought stress induced by PEG-6000. The bioinformatics analysis showed that the open reading frame (ORF) of CsNAC30 is 1254 bp, encoding a protein consisting of 417 amino acids. This protein is relatively unstable and hydrophilic, lacks transmembrane regions, and contains 49 phosphorylation sites. It also features a conserved NAM domain at the N-terminus. Homology and phylogenetic analyses indicate that CsNAC30 is a member of the NAC transcription factor family and is most closely related to the melon CMeNAC105 and the winter melon BhNAC96. Quantitative real-time PCR (qPCR) revealed that CsNAC30 expression is induced by drought stress, with relative levels increasing in both leaves and roots following PEG treatment. Expression peaked at 6 h in leaves and 9 h in roots before decreasing. These findings suggest that CsNAC30 plays a role in the cucumber’s response to drought stress.https://www.tandfonline.com/doi/10.1080/13102818.2024.2425692CucumberNAC transcription factordrought stressbioinformatics |
| spellingShingle | Lei Gong Linna Qian Chengcheng Shen Yuping Wei Wenjiao Wang Expression analysis of CsNAC30 gene in cucumber under drought stress Biotechnology & Biotechnological Equipment Cucumber NAC transcription factor drought stress bioinformatics |
| title | Expression analysis of CsNAC30 gene in cucumber under drought stress |
| title_full | Expression analysis of CsNAC30 gene in cucumber under drought stress |
| title_fullStr | Expression analysis of CsNAC30 gene in cucumber under drought stress |
| title_full_unstemmed | Expression analysis of CsNAC30 gene in cucumber under drought stress |
| title_short | Expression analysis of CsNAC30 gene in cucumber under drought stress |
| title_sort | expression analysis of csnac30 gene in cucumber under drought stress |
| topic | Cucumber NAC transcription factor drought stress bioinformatics |
| url | https://www.tandfonline.com/doi/10.1080/13102818.2024.2425692 |
| work_keys_str_mv | AT leigong expressionanalysisofcsnac30geneincucumberunderdroughtstress AT linnaqian expressionanalysisofcsnac30geneincucumberunderdroughtstress AT chengchengshen expressionanalysisofcsnac30geneincucumberunderdroughtstress AT yupingwei expressionanalysisofcsnac30geneincucumberunderdroughtstress AT wenjiaowang expressionanalysisofcsnac30geneincucumberunderdroughtstress |