Assessment of Serum Biochemical Indices of Albino Rats Induced with Naja nigricollis Venom and Treated with Parkia biglobosa Stem Bark

Assessments of Serum Biochemical Indices of Albino Rats induced with Naja nigricollis venom treated with Parkia biglobosa stem bark were conducted and evaluated. A total of 42 healthy adult Albino rats of both sexes and average weight 153 -275g were used for the experiment. The venom was collected...

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Main Author: Keta, J.N.
Format: Article
Language:English
Published: Umaru Musa Yar'adua University, Katsina, Nigeria 2017-06-01
Series:UMYU Journal of Microbiology Research
Subjects:
Online Access:https://ujmr.umyu.edu.ng/index.php/ujmr/article/view/323
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author Keta, J.N.
author_facet Keta, J.N.
author_sort Keta, J.N.
collection DOAJ
description Assessments of Serum Biochemical Indices of Albino Rats induced with Naja nigricollis venom treated with Parkia biglobosa stem bark were conducted and evaluated. A total of 42 healthy adult Albino rats of both sexes and average weight 153 -275g were used for the experiment. The venom was collected in low light condition at ambient temperature by using a short acting general anesthesia (Halothane) and kept at 4oC. The rats were injected with venom at 0.04 mg followed by conventional anti venom (1 ml) and the plant extract were administered at different doses of 300, 500 and 700 mg/kg respectively. The animals were scarified, and 5ml of blood samples were collected from each rat within 24hrs and tested for ALP, ALT, AST, total protein and Albumin. The LD50 was determined using Lorke’s method and found to be greater than 500mg/kg per oral. The results showed increased and decreased in ALT, ALP and AST while total protein and Albumin were normal. The stem bark extract of Parkia biglobosaa can be said to possess anti-snake venom activity which neutralize the toxic effects of N. nigricollis venom. The potent Snake venom neutralizing capacity of this plant extract is recommended for therapeutic purpose in case of snake bite.  
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spelling doaj-art-11ebdbef13fb4ffa9bcf88304187a7f52025-08-20T02:07:05ZengUmaru Musa Yar'adua University, Katsina, NigeriaUMYU Journal of Microbiology Research2616-06682814-18222017-06-012110.47430/ujmr.1721.038323Assessment of Serum Biochemical Indices of Albino Rats Induced with Naja nigricollis Venom and Treated with Parkia biglobosa Stem BarkKeta, J.N.0Department of Biological Sciences, Kebbi State University of Science and Technology, Aleiro, Kebbi State Assessments of Serum Biochemical Indices of Albino Rats induced with Naja nigricollis venom treated with Parkia biglobosa stem bark were conducted and evaluated. A total of 42 healthy adult Albino rats of both sexes and average weight 153 -275g were used for the experiment. The venom was collected in low light condition at ambient temperature by using a short acting general anesthesia (Halothane) and kept at 4oC. The rats were injected with venom at 0.04 mg followed by conventional anti venom (1 ml) and the plant extract were administered at different doses of 300, 500 and 700 mg/kg respectively. The animals were scarified, and 5ml of blood samples were collected from each rat within 24hrs and tested for ALP, ALT, AST, total protein and Albumin. The LD50 was determined using Lorke’s method and found to be greater than 500mg/kg per oral. The results showed increased and decreased in ALT, ALP and AST while total protein and Albumin were normal. The stem bark extract of Parkia biglobosaa can be said to possess anti-snake venom activity which neutralize the toxic effects of N. nigricollis venom. The potent Snake venom neutralizing capacity of this plant extract is recommended for therapeutic purpose in case of snake bite.   https://ujmr.umyu.edu.ng/index.php/ujmr/article/view/323Naja nigricollis venonRatsBiochemical indicesParkia biglobosaStem bark
spellingShingle Keta, J.N.
Assessment of Serum Biochemical Indices of Albino Rats Induced with Naja nigricollis Venom and Treated with Parkia biglobosa Stem Bark
UMYU Journal of Microbiology Research
Naja nigricollis venon
Rats
Biochemical indices
Parkia biglobosa
Stem bark
title Assessment of Serum Biochemical Indices of Albino Rats Induced with Naja nigricollis Venom and Treated with Parkia biglobosa Stem Bark
title_full Assessment of Serum Biochemical Indices of Albino Rats Induced with Naja nigricollis Venom and Treated with Parkia biglobosa Stem Bark
title_fullStr Assessment of Serum Biochemical Indices of Albino Rats Induced with Naja nigricollis Venom and Treated with Parkia biglobosa Stem Bark
title_full_unstemmed Assessment of Serum Biochemical Indices of Albino Rats Induced with Naja nigricollis Venom and Treated with Parkia biglobosa Stem Bark
title_short Assessment of Serum Biochemical Indices of Albino Rats Induced with Naja nigricollis Venom and Treated with Parkia biglobosa Stem Bark
title_sort assessment of serum biochemical indices of albino rats induced with naja nigricollis venom and treated with parkia biglobosa stem bark
topic Naja nigricollis venon
Rats
Biochemical indices
Parkia biglobosa
Stem bark
url https://ujmr.umyu.edu.ng/index.php/ujmr/article/view/323
work_keys_str_mv AT ketajn assessmentofserumbiochemicalindicesofalbinoratsinducedwithnajanigricollisvenomandtreatedwithparkiabiglobosastembark