Antimicrobial Susceptibility and Genetic Characterisation of Burkholderia pseudomallei Isolated from Malaysian Patients
Burkholderia pseudomallei, the causative agent of melioidosis, is intrinsically resistant to many antibiotics. Ceftazidime (CAZ), the synthetic β-lactam, is normally used as the first-line antibiotic therapy for treatment of melioidosis. However, acquired CAZ resistance can develop in vivo during tr...
Saved in:
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2014-01-01
|
| Series: | The Scientific World Journal |
| Online Access: | http://dx.doi.org/10.1155/2014/132971 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850236650516381696 |
|---|---|
| author | Yalda Khosravi Kumutha Malar Vellasamy Vanitha Mariappan Shet-Lee Ng Jamuna Vadivelu |
| author_facet | Yalda Khosravi Kumutha Malar Vellasamy Vanitha Mariappan Shet-Lee Ng Jamuna Vadivelu |
| author_sort | Yalda Khosravi |
| collection | DOAJ |
| description | Burkholderia pseudomallei, the causative agent of melioidosis, is intrinsically resistant to many antibiotics. Ceftazidime (CAZ), the synthetic β-lactam, is normally used as the first-line antibiotic therapy for treatment of melioidosis. However, acquired CAZ resistance can develop in vivo during treatment with CAZ, leading to mortality if therapy is not switched to a different antibiotic(s) in a timely manner. In this study, susceptibilities of 81 B. pseudomallei isolates to nine different antimicrobial agents were determined using the disk diffusion method, broth microdilution test and Etest. Highest percentage of susceptibility was demonstrated to CAZ, amoxicillin/clavulanic acid, meropenem, imipenem, and trimethoprim/sulfamethoxazole. Although these drugs demonstrated the highest percentage of susceptibility in B. pseudomallei, the overall results underline the importance of the emergence of resistance in this organism. PCR results showed that, of the 81 B. pseudomallei, six multidrug resistant (MDR) isolates carried bpeB, amrB, and BPSS1119 and penA genes. Genotyping of the isolates using random amplified polymorphic DNA analysis showed six different PCR fingerprinting patterns generated from the six MDR isolates clusters (A) and eight PCR fingerprinting patterns generated for the remaining 75 non-MDR isolates clusters (B). |
| format | Article |
| id | doaj-art-11e8b44a7c0f4cfc80cc311b32c8c7d9 |
| institution | OA Journals |
| issn | 2356-6140 1537-744X |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | The Scientific World Journal |
| spelling | doaj-art-11e8b44a7c0f4cfc80cc311b32c8c7d92025-08-20T02:01:55ZengWileyThe Scientific World Journal2356-61401537-744X2014-01-01201410.1155/2014/132971132971Antimicrobial Susceptibility and Genetic Characterisation of Burkholderia pseudomallei Isolated from Malaysian PatientsYalda Khosravi0Kumutha Malar Vellasamy1Vanitha Mariappan2Shet-Lee Ng3Jamuna Vadivelu4Tropical Infectious Disease Research and Education Center (TIDREC), Department of Medical Microbiology, Faculty of Medicine, University of Malaya, 50603 Lembah Pantai, Kuala Lumpur, MalaysiaTropical Infectious Disease Research and Education Center (TIDREC), Department of Medical Microbiology, Faculty of Medicine, University of Malaya, 50603 Lembah Pantai, Kuala Lumpur, MalaysiaTropical Infectious Disease Research and Education Center (TIDREC), Department of Medical Microbiology, Faculty of Medicine, University of Malaya, 50603 Lembah Pantai, Kuala Lumpur, MalaysiaTropical Infectious Disease Research and Education Center (TIDREC), Department of Medical Microbiology, Faculty of Medicine, University of Malaya, 50603 Lembah Pantai, Kuala Lumpur, MalaysiaTropical Infectious Disease Research and Education Center (TIDREC), Department of Medical Microbiology, Faculty of Medicine, University of Malaya, 50603 Lembah Pantai, Kuala Lumpur, MalaysiaBurkholderia pseudomallei, the causative agent of melioidosis, is intrinsically resistant to many antibiotics. Ceftazidime (CAZ), the synthetic β-lactam, is normally used as the first-line antibiotic therapy for treatment of melioidosis. However, acquired CAZ resistance can develop in vivo during treatment with CAZ, leading to mortality if therapy is not switched to a different antibiotic(s) in a timely manner. In this study, susceptibilities of 81 B. pseudomallei isolates to nine different antimicrobial agents were determined using the disk diffusion method, broth microdilution test and Etest. Highest percentage of susceptibility was demonstrated to CAZ, amoxicillin/clavulanic acid, meropenem, imipenem, and trimethoprim/sulfamethoxazole. Although these drugs demonstrated the highest percentage of susceptibility in B. pseudomallei, the overall results underline the importance of the emergence of resistance in this organism. PCR results showed that, of the 81 B. pseudomallei, six multidrug resistant (MDR) isolates carried bpeB, amrB, and BPSS1119 and penA genes. Genotyping of the isolates using random amplified polymorphic DNA analysis showed six different PCR fingerprinting patterns generated from the six MDR isolates clusters (A) and eight PCR fingerprinting patterns generated for the remaining 75 non-MDR isolates clusters (B).http://dx.doi.org/10.1155/2014/132971 |
| spellingShingle | Yalda Khosravi Kumutha Malar Vellasamy Vanitha Mariappan Shet-Lee Ng Jamuna Vadivelu Antimicrobial Susceptibility and Genetic Characterisation of Burkholderia pseudomallei Isolated from Malaysian Patients The Scientific World Journal |
| title | Antimicrobial Susceptibility and Genetic Characterisation of Burkholderia pseudomallei Isolated from Malaysian Patients |
| title_full | Antimicrobial Susceptibility and Genetic Characterisation of Burkholderia pseudomallei Isolated from Malaysian Patients |
| title_fullStr | Antimicrobial Susceptibility and Genetic Characterisation of Burkholderia pseudomallei Isolated from Malaysian Patients |
| title_full_unstemmed | Antimicrobial Susceptibility and Genetic Characterisation of Burkholderia pseudomallei Isolated from Malaysian Patients |
| title_short | Antimicrobial Susceptibility and Genetic Characterisation of Burkholderia pseudomallei Isolated from Malaysian Patients |
| title_sort | antimicrobial susceptibility and genetic characterisation of burkholderia pseudomallei isolated from malaysian patients |
| url | http://dx.doi.org/10.1155/2014/132971 |
| work_keys_str_mv | AT yaldakhosravi antimicrobialsusceptibilityandgeneticcharacterisationofburkholderiapseudomalleiisolatedfrommalaysianpatients AT kumuthamalarvellasamy antimicrobialsusceptibilityandgeneticcharacterisationofburkholderiapseudomalleiisolatedfrommalaysianpatients AT vanithamariappan antimicrobialsusceptibilityandgeneticcharacterisationofburkholderiapseudomalleiisolatedfrommalaysianpatients AT shetleeng antimicrobialsusceptibilityandgeneticcharacterisationofburkholderiapseudomalleiisolatedfrommalaysianpatients AT jamunavadivelu antimicrobialsusceptibilityandgeneticcharacterisationofburkholderiapseudomalleiisolatedfrommalaysianpatients |