Investigating the therapeutic effect of folic acid conjugated ZnO nanoparticles on human triple negative breast cancer cell line
Triple-negative breast cancer (TNBC) accounts for 15-20% of all invasive breast cancers and has a poor prognosis. ZnO NPs are promising anti-cancer agents. Moreover, folate Alpha receptor (FRa) is a potential biomarker and therapeutic target because it is significantly expressed in TNBC. Therefore,...
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Engineering Society for Corrosion, Belgrade
2023-06-01
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| Series: | Zaštita Materijala |
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| Online Access: | https://www.zastita-materijala.org/index.php/home/article/view/104 |
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| author | Shiva Sabzandam Masoumeh Zahmatkeshan Moein Adel Mehrad Mehrdadian Farzaneh Saliminia Fariba Esmaeili |
| author_facet | Shiva Sabzandam Masoumeh Zahmatkeshan Moein Adel Mehrad Mehrdadian Farzaneh Saliminia Fariba Esmaeili |
| author_sort | Shiva Sabzandam |
| collection | DOAJ |
| description | Triple-negative breast cancer (TNBC) accounts for 15-20% of all invasive breast cancers and has a poor prognosis. ZnO NPs are promising anti-cancer agents. Moreover, folate Alpha receptor (FRa) is a potential biomarker and therapeutic target because it is significantly expressed in TNBC. Therefore, ZnO and folic acid-conjugated ZnO (F-ZnO) NPs were synthesized by the solgel method. NPs were characterized by DLS, zeta potential, TEM, FTIR, and ICP-MS. FA-ZnO NPs had a mean diameter of 20 ± 2 nm and a surface charge of -15 mV, while ZnO NPs had a mean diameter of 40 ± 5 nm and a surface charge of -5 mV. The MTT assay and trypan blue test, respectively, were used to determine the cytotoxicity and viability percentage of ZnO and F-ZnO NPs at different concentrations of 2, 4, 8, 16, 32, 64 and 128 µg/mL for 12, 24, 48 and 72 hours (h) on the human TNBC cell line MDA-MB-231. The results indicated that both ZnO and F-ZnO NPs significantly reduced the viability of the cancer cells in a dose-dependent and time-dependent manner (p |
| format | Article |
| id | doaj-art-11e73dcb29164d82a8072d91bb1880c9 |
| institution | DOAJ |
| issn | 0351-9465 2466-2585 |
| language | English |
| publishDate | 2023-06-01 |
| publisher | Engineering Society for Corrosion, Belgrade |
| record_format | Article |
| series | Zaštita Materijala |
| spelling | doaj-art-11e73dcb29164d82a8072d91bb1880c92025-08-20T03:17:09ZengEngineering Society for Corrosion, BelgradeZaštita Materijala0351-94652466-25852023-06-0164221322210.5937/zasmat2302213S103Investigating the therapeutic effect of folic acid conjugated ZnO nanoparticles on human triple negative breast cancer cell lineShiva Sabzandam0Masoumeh Zahmatkeshan1Moein Adel2Mehrad Mehrdadian3Farzaneh Saliminia4Fariba Esmaeili5Islamic Azad University, Tehran Medical Sciences, Faculty of Advanced Sciences and Technology, Department of Medical Nanotechnology, TehranIran University of Medical Sciences, Cellular and Molecular Research Center, Tehran, Iran + Iran University of Medical Sciences, Faculty of Advanced Technologies in Medicine, Department of Medical Nanotechnology, Tehran, IranTabriz University of Medical Sciences, Department of Medical Nanotechnology, Tabriz, IranIran University of Medical Sciences, School of Medicine-International Campus, Tehran, IranIran University of Medical Sciences, Faculty of Advanced Technologies in Medicine, Department of Medical Nanotechnology, Tehran, IranTehran University of Medical Sciences, School of Advanced Technologies in Medicine, Department of Medical Nanotechnology, Tehran, IranTriple-negative breast cancer (TNBC) accounts for 15-20% of all invasive breast cancers and has a poor prognosis. ZnO NPs are promising anti-cancer agents. Moreover, folate Alpha receptor (FRa) is a potential biomarker and therapeutic target because it is significantly expressed in TNBC. Therefore, ZnO and folic acid-conjugated ZnO (F-ZnO) NPs were synthesized by the solgel method. NPs were characterized by DLS, zeta potential, TEM, FTIR, and ICP-MS. FA-ZnO NPs had a mean diameter of 20 ± 2 nm and a surface charge of -15 mV, while ZnO NPs had a mean diameter of 40 ± 5 nm and a surface charge of -5 mV. The MTT assay and trypan blue test, respectively, were used to determine the cytotoxicity and viability percentage of ZnO and F-ZnO NPs at different concentrations of 2, 4, 8, 16, 32, 64 and 128 µg/mL for 12, 24, 48 and 72 hours (h) on the human TNBC cell line MDA-MB-231. The results indicated that both ZnO and F-ZnO NPs significantly reduced the viability of the cancer cells in a dose-dependent and time-dependent manner (phttps://www.zastita-materijala.org/index.php/home/article/view/104nanoparticlezinc oxidefolic acidtriple negative breast cancer |
| spellingShingle | Shiva Sabzandam Masoumeh Zahmatkeshan Moein Adel Mehrad Mehrdadian Farzaneh Saliminia Fariba Esmaeili Investigating the therapeutic effect of folic acid conjugated ZnO nanoparticles on human triple negative breast cancer cell line Zaštita Materijala nanoparticle zinc oxide folic acid triple negative breast cancer |
| title | Investigating the therapeutic effect of folic acid conjugated ZnO nanoparticles on human triple negative breast cancer cell line |
| title_full | Investigating the therapeutic effect of folic acid conjugated ZnO nanoparticles on human triple negative breast cancer cell line |
| title_fullStr | Investigating the therapeutic effect of folic acid conjugated ZnO nanoparticles on human triple negative breast cancer cell line |
| title_full_unstemmed | Investigating the therapeutic effect of folic acid conjugated ZnO nanoparticles on human triple negative breast cancer cell line |
| title_short | Investigating the therapeutic effect of folic acid conjugated ZnO nanoparticles on human triple negative breast cancer cell line |
| title_sort | investigating the therapeutic effect of folic acid conjugated zno nanoparticles on human triple negative breast cancer cell line |
| topic | nanoparticle zinc oxide folic acid triple negative breast cancer |
| url | https://www.zastita-materijala.org/index.php/home/article/view/104 |
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