Identification of a potential prognostic panel of biomarkers for stratification of head and neck squamous cell carcinoma based on HPV status and TP53 mutational status

Head and Neck Squamous Cell Carcinoma (HNSCC) is a malignant cancer with poor prognosis. Currently, the prognosis of HNSCC is determined by clinical and histopathological criteria. This work focused on identifying a panel of genes that have the potential to be used for prognosis of HNSCC and to impr...

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Main Authors: Oriana Barros, Rita Ferreira, Vito G. D'Agostino, Francisco Amado, Lucio Santos, Rui Vitorino
Format: Article
Language:English
Published: Elsevier 2023-03-01
Series:Oral Oncology Reports
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Online Access:http://www.sciencedirect.com/science/article/pii/S2772906023000080
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author Oriana Barros
Rita Ferreira
Vito G. D'Agostino
Francisco Amado
Lucio Santos
Rui Vitorino
author_facet Oriana Barros
Rita Ferreira
Vito G. D'Agostino
Francisco Amado
Lucio Santos
Rui Vitorino
author_sort Oriana Barros
collection DOAJ
description Head and Neck Squamous Cell Carcinoma (HNSCC) is a malignant cancer with poor prognosis. Currently, the prognosis of HNSCC is determined by clinical and histopathological criteria. This work focused on identifying a panel of genes that have the potential to be used for prognosis of HNSCC and to improve patient stratification for treatment. To this end, a bibliometric analysis (VosViewer) was applied to identify candidate genes that were further characterized by applying several bioinformatics tools (UALCAN, ToPP). The prognostic potential of the genes of interest was evaluated using the univariate and the multivariate Cox proportional regression models and the transcriptional expression analysis among HNSCC and normal tissues. In HNSCC, the transcriptional levels of candidate genes, were analyzed in HPV-driven HNSCC, HPV-non-driven HNSCC, TP53-mutant HNSCC and TP53-nonmutant HNSCC for selecting the best set of genes for discrimination of HNSCC based on both HPV status and TP53 mutational status. These analyses revealed a signature based on four genes with greater HNSCC prognostic potential: CDKN2A, TGFB1, CD44 and MMP9, being p16 the sole biomarker currently tested. In the future, a molecular signature could facilitate the stratification of patients into high- and low-risk groups as well the wiser adjustment of therapies to each individual response allowing a personalized treatment.
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spelling doaj-art-11d2e2d4e20b420d830cbfe2abb23d3c2025-08-20T02:50:20ZengElsevierOral Oncology Reports2772-90602023-03-01510001810.1016/j.oor.2023.100018Identification of a potential prognostic panel of biomarkers for stratification of head and neck squamous cell carcinoma based on HPV status and TP53 mutational statusOriana Barros0Rita Ferreira1Vito G. D'Agostino2Francisco Amado3Lucio Santos4Rui Vitorino5Department of Medical Sciences, Institute of Biomedicine iBiMED, University of Aveiro, 3810-193, Aveiro, Portugal; Experimental Pathology and Therapeutics Group, Research Center of IPO Porto (CI-IPOP), RISE@CI-IPOP (Health Research Network) and Surgical Department of Portuguese Oncology Institute of Porto (IPO Porto), Porto Comprehensive Cancer Center (Porto.CCC), PortugalLAQV-REQUIMTE, Department of Chemistry, University of Aveiro, Campus Universitário de Santiago, 3810-193, Aveiro, PortugalDepartment of Cellular, Computational and Integrative Biology (CIBIO), University of Trento, Via Sommarive 9, 38123, Trento, ItalyLAQV-REQUIMTE, Department of Chemistry, University of Aveiro, Campus Universitário de Santiago, 3810-193, Aveiro, PortugalExperimental Pathology and Therapeutics Group, Research Center of IPO Porto (CI-IPOP), RISE@CI-IPOP (Health Research Network) and Surgical Department of Portuguese Oncology Institute of Porto (IPO Porto), Porto Comprehensive Cancer Center (Porto.CCC), PortugalDepartment of Medical Sciences, Institute of Biomedicine iBiMED, University of Aveiro, 3810-193, Aveiro, Portugal; LAQV-REQUIMTE, Department of Chemistry, University of Aveiro, Campus Universitário de Santiago, 3810-193, Aveiro, Portugal; UnIC, Department of Surgery and Physiology, Faculty of Medicine, University of Porto, 4200-319, Porto, Portugal; Corresponding author. Department of Medical Sciences, Institute of Biomedicine iBiMED, University of Aveiro, 3810-193, Aveiro, Portugal.Head and Neck Squamous Cell Carcinoma (HNSCC) is a malignant cancer with poor prognosis. Currently, the prognosis of HNSCC is determined by clinical and histopathological criteria. This work focused on identifying a panel of genes that have the potential to be used for prognosis of HNSCC and to improve patient stratification for treatment. To this end, a bibliometric analysis (VosViewer) was applied to identify candidate genes that were further characterized by applying several bioinformatics tools (UALCAN, ToPP). The prognostic potential of the genes of interest was evaluated using the univariate and the multivariate Cox proportional regression models and the transcriptional expression analysis among HNSCC and normal tissues. In HNSCC, the transcriptional levels of candidate genes, were analyzed in HPV-driven HNSCC, HPV-non-driven HNSCC, TP53-mutant HNSCC and TP53-nonmutant HNSCC for selecting the best set of genes for discrimination of HNSCC based on both HPV status and TP53 mutational status. These analyses revealed a signature based on four genes with greater HNSCC prognostic potential: CDKN2A, TGFB1, CD44 and MMP9, being p16 the sole biomarker currently tested. In the future, a molecular signature could facilitate the stratification of patients into high- and low-risk groups as well the wiser adjustment of therapies to each individual response allowing a personalized treatment.http://www.sciencedirect.com/science/article/pii/S2772906023000080Head and neck squamous cell carcinomaPrognosisBiomarkersPrecision medicine
spellingShingle Oriana Barros
Rita Ferreira
Vito G. D'Agostino
Francisco Amado
Lucio Santos
Rui Vitorino
Identification of a potential prognostic panel of biomarkers for stratification of head and neck squamous cell carcinoma based on HPV status and TP53 mutational status
Oral Oncology Reports
Head and neck squamous cell carcinoma
Prognosis
Biomarkers
Precision medicine
title Identification of a potential prognostic panel of biomarkers for stratification of head and neck squamous cell carcinoma based on HPV status and TP53 mutational status
title_full Identification of a potential prognostic panel of biomarkers for stratification of head and neck squamous cell carcinoma based on HPV status and TP53 mutational status
title_fullStr Identification of a potential prognostic panel of biomarkers for stratification of head and neck squamous cell carcinoma based on HPV status and TP53 mutational status
title_full_unstemmed Identification of a potential prognostic panel of biomarkers for stratification of head and neck squamous cell carcinoma based on HPV status and TP53 mutational status
title_short Identification of a potential prognostic panel of biomarkers for stratification of head and neck squamous cell carcinoma based on HPV status and TP53 mutational status
title_sort identification of a potential prognostic panel of biomarkers for stratification of head and neck squamous cell carcinoma based on hpv status and tp53 mutational status
topic Head and neck squamous cell carcinoma
Prognosis
Biomarkers
Precision medicine
url http://www.sciencedirect.com/science/article/pii/S2772906023000080
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