Improvement of the transformation system in Chinese cabbage (Brassica campestris ssp. chinensis Makino)
The binary plasmid vector pBI35S-AMF was transferred into cv. Shanghaiqing of Chinese cabbage pak-choi (Brassica campestris ssp. Mokino chinensis var. communis Tsen et Lee) and cv. Youqing of flowering Chinese cabbage (B. campestris ssp. chinensis Makino var. parachinensis (Bailey) Tsen et Lee) by A...
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| Format: | Article |
| Language: | English |
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Zhejiang University Press
2005-09-01
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| Series: | 浙江大学学报. 农业与生命科学版 |
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| Online Access: | https://www.academax.com/doi/10.3785/1008-9209.2005.05.0529 |
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| author | YU Xiao-lin CAO Jia-shu XU Li-kui QI Wen-wen WANG Xiao-jing |
| author_facet | YU Xiao-lin CAO Jia-shu XU Li-kui QI Wen-wen WANG Xiao-jing |
| author_sort | YU Xiao-lin |
| collection | DOAJ |
| description | The binary plasmid vector pBI35S-AMF was transferred into cv. Shanghaiqing of Chinese cabbage pak-choi (Brassica campestris ssp. Mokino chinensis var. communis Tsen et Lee) and cv. Youqing of flowering Chinese cabbage (B. campestris ssp. chinensis Makino var. parachinensis (Bailey) Tsen et Lee) by Agrobacterium-mediated method. The pivotal factors, which influenced the transformation frequency, were compared, and the parameters of transformation system were optimized. It was showed that selective medium containing 300 mg/L ampicillin, which inhibited growth of Agrobacterium, led to production of more Kan<sup>R</sup> shoots; the time required for preculture and co-culture was three days and two days respectively; and the efficiency of inoculation with LBA4404/pBI35S-AMF was significantly higher than with EHA105/pBI35S-AMF. Molecular analysis to Kan<sup>R</sup> seedlings showed that the frequency of Southern blotting was 82.2% and most of transformants were multi-copies integration. Moreover, the results indicated that the efficiency of transformation system was stable. |
| format | Article |
| id | doaj-art-11cc0ef157764a5f8ca4191fc3522d85 |
| institution | Kabale University |
| issn | 1008-9209 2097-5155 |
| language | English |
| publishDate | 2005-09-01 |
| publisher | Zhejiang University Press |
| record_format | Article |
| series | 浙江大学学报. 农业与生命科学版 |
| spelling | doaj-art-11cc0ef157764a5f8ca4191fc3522d852025-08-20T03:58:18ZengZhejiang University Press浙江大学学报. 农业与生命科学版1008-92092097-51552005-09-013152953410.3785/1008-9209.2005.05.052910089209Improvement of the transformation system in Chinese cabbage (Brassica campestris ssp. chinensis Makino)YU Xiao-linCAO Jia-shuXU Li-kuiQI Wen-wenWANG Xiao-jingThe binary plasmid vector pBI35S-AMF was transferred into cv. Shanghaiqing of Chinese cabbage pak-choi (Brassica campestris ssp. Mokino chinensis var. communis Tsen et Lee) and cv. Youqing of flowering Chinese cabbage (B. campestris ssp. chinensis Makino var. parachinensis (Bailey) Tsen et Lee) by Agrobacterium-mediated method. The pivotal factors, which influenced the transformation frequency, were compared, and the parameters of transformation system were optimized. It was showed that selective medium containing 300 mg/L ampicillin, which inhibited growth of Agrobacterium, led to production of more Kan<sup>R</sup> shoots; the time required for preculture and co-culture was three days and two days respectively; and the efficiency of inoculation with LBA4404/pBI35S-AMF was significantly higher than with EHA105/pBI35S-AMF. Molecular analysis to Kan<sup>R</sup> seedlings showed that the frequency of Southern blotting was 82.2% and most of transformants were multi-copies integration. Moreover, the results indicated that the efficiency of transformation system was stable.https://www.academax.com/doi/10.3785/1008-9209.2005.05.0529Chinese cabbage pak-choiflowering Chinese cabbagegenetic transformationthe frequency of transformation |
| spellingShingle | YU Xiao-lin CAO Jia-shu XU Li-kui QI Wen-wen WANG Xiao-jing Improvement of the transformation system in Chinese cabbage (Brassica campestris ssp. chinensis Makino) 浙江大学学报. 农业与生命科学版 Chinese cabbage pak-choi flowering Chinese cabbage genetic transformation the frequency of transformation |
| title | Improvement of the transformation system in Chinese cabbage (Brassica campestris ssp. chinensis Makino) |
| title_full | Improvement of the transformation system in Chinese cabbage (Brassica campestris ssp. chinensis Makino) |
| title_fullStr | Improvement of the transformation system in Chinese cabbage (Brassica campestris ssp. chinensis Makino) |
| title_full_unstemmed | Improvement of the transformation system in Chinese cabbage (Brassica campestris ssp. chinensis Makino) |
| title_short | Improvement of the transformation system in Chinese cabbage (Brassica campestris ssp. chinensis Makino) |
| title_sort | improvement of the transformation system in chinese cabbage brassica campestris ssp chinensis makino |
| topic | Chinese cabbage pak-choi flowering Chinese cabbage genetic transformation the frequency of transformation |
| url | https://www.academax.com/doi/10.3785/1008-9209.2005.05.0529 |
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