Phenolic Compounds Isolated from Calea uniflora Less. Promote Anti-Inflammatory and Antioxidant Effects in Mice Neutrophils (Ex Vivo) and in Mice Pleurisy Model (In Vivo)
The literature shows that phenolic compounds possess important antioxidant and anti-inflammatory activities; however, the mechanism underlying these effects is not elucidated yet. The genus Calea is used in folk medicine to treat rheumatism, respiratory diseases, and digestive problems. In this cont...
Saved in:
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2019-01-01
|
| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/2019/1468502 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849309310824218624 |
|---|---|
| author | Julia Salvan da Rosa Marcus Vinicius Pereira Dos Santos Nascimento Eduardo Benedetti Parisotto Tamires Cardoso Lima José Roberto Santin Maique Weber Biavatti Ariane Zamoner Eduardo Monguilhott Dalmarco Tânia Silvia Fröde |
| author_facet | Julia Salvan da Rosa Marcus Vinicius Pereira Dos Santos Nascimento Eduardo Benedetti Parisotto Tamires Cardoso Lima José Roberto Santin Maique Weber Biavatti Ariane Zamoner Eduardo Monguilhott Dalmarco Tânia Silvia Fröde |
| author_sort | Julia Salvan da Rosa |
| collection | DOAJ |
| description | The literature shows that phenolic compounds possess important antioxidant and anti-inflammatory activities; however, the mechanism underlying these effects is not elucidated yet. The genus Calea is used in folk medicine to treat rheumatism, respiratory diseases, and digestive problems. In this context, some phenolic compounds were isolated with high purity from Calea uniflora Less. and identified as noreugenin (NRG) and α-hydroxy-butein (AH-BU). The aim of this study was to analyze the effect of these compounds on cell viability, the activity of myeloperoxidase (MPO), and apoptosis of mouse neutrophils using ex vivo tests. Furthermore, the effect of these compounds on the cytokines, interleukin 1 beta (IL-1β), interleukin 17A (IL-17A), and interleukin 10 (IL-10), and oxidative stress was investigated by analyzing lipid peroxidation (the concentration of thiobarbituric acid reactive substances (TBARS)) and activities of antioxidant enzymes, superoxide dismutase (SOD), catalase (CAT), and glutathione S-transferase (GST), using a murine model of neutrophilic inflammation. The NRG and AH-BU reduce MPO activity and increase neutrophil apoptosis (p<0.05). These compounds reduced the generation of oxygen reactive species and IL-1β and IL-17A levels but increased IL-10 levels (p<0.05). This study demonstrated that NRG and AH-BU show a significant anti-inflammatory effect by inhibiting the MPO activity and increasing neutrophil apoptosis in primary cultures of mouse neutrophils. These effects were at least partially associated with blocking reactive species generation, inhibiting IL-1β and IL-17A, and increasing IL-10 levels. |
| format | Article |
| id | doaj-art-11c53bfd5d4d4b32b2563f0d64f6ea33 |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2019-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-11c53bfd5d4d4b32b2563f0d64f6ea332025-08-20T03:54:12ZengWileyMediators of Inflammation0962-93511466-18612019-01-01201910.1155/2019/14685021468502Phenolic Compounds Isolated from Calea uniflora Less. Promote Anti-Inflammatory and Antioxidant Effects in Mice Neutrophils (Ex Vivo) and in Mice Pleurisy Model (In Vivo)Julia Salvan da Rosa0Marcus Vinicius Pereira Dos Santos Nascimento1Eduardo Benedetti Parisotto2Tamires Cardoso Lima3José Roberto Santin4Maique Weber Biavatti5Ariane Zamoner6Eduardo Monguilhott Dalmarco7Tânia Silvia Fröde8Graduate Course of Pharmacy, Center of Health Sciences, Federal University of Santa Catarina (UFSC), Florianópolis, SC, BrazilGraduate Course of Pharmacy, Center of Health Sciences, Federal University of Santa Catarina (UFSC), Florianópolis, SC, BrazilDepartment of Biochemistry, Center of Biological Sciences, Federal University of Santa Catarina (UFSC), Florianópolis, SC, BrazilDepartment of Pharmaceutical Sciences, Center of Health Sciences, Federal University of Santa Catarina (UFSC), Florianópolis, SC, BrazilNúcleo de Investigações Químico-Farmacêuticas, University of Vale do Itajaí (UNIVALI), Itajaí, SC, BrazilDepartment of Pharmaceutical Sciences, Center of Health Sciences, Federal University of Santa Catarina (UFSC), Florianópolis, SC, BrazilDepartment of Biochemistry, Center of Biological Sciences, Federal University of Santa Catarina (UFSC), Florianópolis, SC, BrazilGraduate Course of Pharmacy, Center of Health Sciences, Federal University of Santa Catarina (UFSC), Florianópolis, SC, BrazilGraduate Course of Pharmacy, Center of Health Sciences, Federal University of Santa Catarina (UFSC), Florianópolis, SC, BrazilThe literature shows that phenolic compounds possess important antioxidant and anti-inflammatory activities; however, the mechanism underlying these effects is not elucidated yet. The genus Calea is used in folk medicine to treat rheumatism, respiratory diseases, and digestive problems. In this context, some phenolic compounds were isolated with high purity from Calea uniflora Less. and identified as noreugenin (NRG) and α-hydroxy-butein (AH-BU). The aim of this study was to analyze the effect of these compounds on cell viability, the activity of myeloperoxidase (MPO), and apoptosis of mouse neutrophils using ex vivo tests. Furthermore, the effect of these compounds on the cytokines, interleukin 1 beta (IL-1β), interleukin 17A (IL-17A), and interleukin 10 (IL-10), and oxidative stress was investigated by analyzing lipid peroxidation (the concentration of thiobarbituric acid reactive substances (TBARS)) and activities of antioxidant enzymes, superoxide dismutase (SOD), catalase (CAT), and glutathione S-transferase (GST), using a murine model of neutrophilic inflammation. The NRG and AH-BU reduce MPO activity and increase neutrophil apoptosis (p<0.05). These compounds reduced the generation of oxygen reactive species and IL-1β and IL-17A levels but increased IL-10 levels (p<0.05). This study demonstrated that NRG and AH-BU show a significant anti-inflammatory effect by inhibiting the MPO activity and increasing neutrophil apoptosis in primary cultures of mouse neutrophils. These effects were at least partially associated with blocking reactive species generation, inhibiting IL-1β and IL-17A, and increasing IL-10 levels.http://dx.doi.org/10.1155/2019/1468502 |
| spellingShingle | Julia Salvan da Rosa Marcus Vinicius Pereira Dos Santos Nascimento Eduardo Benedetti Parisotto Tamires Cardoso Lima José Roberto Santin Maique Weber Biavatti Ariane Zamoner Eduardo Monguilhott Dalmarco Tânia Silvia Fröde Phenolic Compounds Isolated from Calea uniflora Less. Promote Anti-Inflammatory and Antioxidant Effects in Mice Neutrophils (Ex Vivo) and in Mice Pleurisy Model (In Vivo) Mediators of Inflammation |
| title | Phenolic Compounds Isolated from Calea uniflora Less. Promote Anti-Inflammatory and Antioxidant Effects in Mice Neutrophils (Ex Vivo) and in Mice Pleurisy Model (In Vivo) |
| title_full | Phenolic Compounds Isolated from Calea uniflora Less. Promote Anti-Inflammatory and Antioxidant Effects in Mice Neutrophils (Ex Vivo) and in Mice Pleurisy Model (In Vivo) |
| title_fullStr | Phenolic Compounds Isolated from Calea uniflora Less. Promote Anti-Inflammatory and Antioxidant Effects in Mice Neutrophils (Ex Vivo) and in Mice Pleurisy Model (In Vivo) |
| title_full_unstemmed | Phenolic Compounds Isolated from Calea uniflora Less. Promote Anti-Inflammatory and Antioxidant Effects in Mice Neutrophils (Ex Vivo) and in Mice Pleurisy Model (In Vivo) |
| title_short | Phenolic Compounds Isolated from Calea uniflora Less. Promote Anti-Inflammatory and Antioxidant Effects in Mice Neutrophils (Ex Vivo) and in Mice Pleurisy Model (In Vivo) |
| title_sort | phenolic compounds isolated from calea uniflora less promote anti inflammatory and antioxidant effects in mice neutrophils ex vivo and in mice pleurisy model in vivo |
| url | http://dx.doi.org/10.1155/2019/1468502 |
| work_keys_str_mv | AT juliasalvandarosa phenoliccompoundsisolatedfromcaleaunifloralesspromoteantiinflammatoryandantioxidanteffectsinmiceneutrophilsexvivoandinmicepleurisymodelinvivo AT marcusviniciuspereiradossantosnascimento phenoliccompoundsisolatedfromcaleaunifloralesspromoteantiinflammatoryandantioxidanteffectsinmiceneutrophilsexvivoandinmicepleurisymodelinvivo AT eduardobenedettiparisotto phenoliccompoundsisolatedfromcaleaunifloralesspromoteantiinflammatoryandantioxidanteffectsinmiceneutrophilsexvivoandinmicepleurisymodelinvivo AT tamirescardosolima phenoliccompoundsisolatedfromcaleaunifloralesspromoteantiinflammatoryandantioxidanteffectsinmiceneutrophilsexvivoandinmicepleurisymodelinvivo AT joserobertosantin phenoliccompoundsisolatedfromcaleaunifloralesspromoteantiinflammatoryandantioxidanteffectsinmiceneutrophilsexvivoandinmicepleurisymodelinvivo AT maiqueweberbiavatti phenoliccompoundsisolatedfromcaleaunifloralesspromoteantiinflammatoryandantioxidanteffectsinmiceneutrophilsexvivoandinmicepleurisymodelinvivo AT arianezamoner phenoliccompoundsisolatedfromcaleaunifloralesspromoteantiinflammatoryandantioxidanteffectsinmiceneutrophilsexvivoandinmicepleurisymodelinvivo AT eduardomonguilhottdalmarco phenoliccompoundsisolatedfromcaleaunifloralesspromoteantiinflammatoryandantioxidanteffectsinmiceneutrophilsexvivoandinmicepleurisymodelinvivo AT taniasilviafrode phenoliccompoundsisolatedfromcaleaunifloralesspromoteantiinflammatoryandantioxidanteffectsinmiceneutrophilsexvivoandinmicepleurisymodelinvivo |