Bio-fabrication of Cichorium intybus L. root aqueous extract mediated ZnO nanoparticle (CIRAE-ZnO NP) for its promising therapeutic applications

This research aims to determine the in-vitro antioxidant, antidiabetic, hemocompatibility, and anticancer properties of phyto-synthesized ZnO NPs using root aqueous extract of Cichorium intybus (CIRAE-ZnO NPs). The CIRAE-ZnO NPs were subjected to various characterization techniques (UV, FTIR, XRD, z...

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Bibliographic Details
Main Authors: Nayan Kumar Sishu, Chinnadurai Immanuel Selvaraj
Format: Article
Language:English
Published: Taylor & Francis Group 2025-12-01
Series:Green Chemistry Letters and Reviews
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Online Access:https://www.tandfonline.com/doi/10.1080/17518253.2025.2489461
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Summary:This research aims to determine the in-vitro antioxidant, antidiabetic, hemocompatibility, and anticancer properties of phyto-synthesized ZnO NPs using root aqueous extract of Cichorium intybus (CIRAE-ZnO NPs). The CIRAE-ZnO NPs were subjected to various characterization techniques (UV, FTIR, XRD, zeta potential, DLS, FE-SEM, and HR-TEM). The HR-TEM analysis showed the average size of ZnO NPs as 26.66 ± 0.84 nm. The CIRAE-ZnO NPs were quasi-spherical with IC50 values in DPPH, phosphomolybdate, Fe chelating, and anti-inflammatory assays as 60.63 ± 0.4, 62.11 ± 0.14, 58.79 ± 0.5, and 52.63 ± 0.3 μg/mL, respectively. The CIRAE-ZnO NPs exhibited less haemolytic activity (0.80 mg/mL) and moderate thrombolytic activity (56.57%). The IC50 value for CIRAE-ZnO NPs in A549 and 3T3-L1 cells cytotoxicity was 26.56 ± 0.7 and 51.62 ± 0.36 μg/mL, respectively. The CIRAE-ZnO NPs had an LC50 reading of 0.506 μg/mL, which renders them at a lower risk to Artemia salina. The CIRAE-ZnO NPs exhibited antidiabetic activity with IC50 values of 62.63 ± 0.12 and 41.74 ± 0.15 µg/mL for α-glucosidase and α-amylase inhibitory activity, respectively. The NPs showed promising results in glucose uptake, glucose diffusion assay, and amylolysis kinetics. Thus, there are several uses for CIRAE-ZnO NPs in biomedical research.
ISSN:1751-8253
1751-7192