New insights into the structure of (1→3,1→6)-β-D-glucan side chains in the Candida glabrata cell wall.
β-Glucan is a (1→3)-β-linked glucose polymer with (1→6)-β-linked side chains and a major component of fungal cell walls. β-Glucans provide structural integrity to the fungal cell wall. The nature of the (1-6)-β-linked side chain structure of fungal (1→3,1→6)-β-D-glucans has been very difficult to el...
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2011-01-01
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| Series: | PLoS ONE |
| Online Access: | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0027614&type=printable |
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| author | Douglas W Lowman Lara J West Daniel W Bearden Michael F Wempe Trevor D Power Harry E Ensley Ken Haynes David L Williams Michael D Kruppa |
| author_facet | Douglas W Lowman Lara J West Daniel W Bearden Michael F Wempe Trevor D Power Harry E Ensley Ken Haynes David L Williams Michael D Kruppa |
| author_sort | Douglas W Lowman |
| collection | DOAJ |
| description | β-Glucan is a (1→3)-β-linked glucose polymer with (1→6)-β-linked side chains and a major component of fungal cell walls. β-Glucans provide structural integrity to the fungal cell wall. The nature of the (1-6)-β-linked side chain structure of fungal (1→3,1→6)-β-D-glucans has been very difficult to elucidate. Herein, we report the first detailed structural characterization of the (1→6)-β-linked side chains of Candida glabrata using high-field NMR. The (1→6)-β-linked side chains have an average length of 4 to 5 repeat units spaced every 21 repeat units along the (1→3)-linked polymer backbone. Computer modeling suggests that the side chains have a bent curve structure that allows for a flexible interconnection with parallel (1→3)-β-D-glucan polymers, and/or as a point of attachment for proteins. Based on these observations we propose new approaches to how (1→6)-β-linked side chains interconnect with neighboring glucan polymers in a manner that maximizes fungal cell wall strength, while also allowing for flexibility, or plasticity. |
| format | Article |
| id | doaj-art-11abf6702dcd422bb250e4f7c2eda27d |
| institution | Kabale University |
| issn | 1932-6203 |
| language | English |
| publishDate | 2011-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj-art-11abf6702dcd422bb250e4f7c2eda27d2025-08-20T03:25:08ZengPublic Library of Science (PLoS)PLoS ONE1932-62032011-01-01611e2761410.1371/journal.pone.0027614New insights into the structure of (1→3,1→6)-β-D-glucan side chains in the Candida glabrata cell wall.Douglas W LowmanLara J WestDaniel W BeardenMichael F WempeTrevor D PowerHarry E EnsleyKen HaynesDavid L WilliamsMichael D Kruppaβ-Glucan is a (1→3)-β-linked glucose polymer with (1→6)-β-linked side chains and a major component of fungal cell walls. β-Glucans provide structural integrity to the fungal cell wall. The nature of the (1-6)-β-linked side chain structure of fungal (1→3,1→6)-β-D-glucans has been very difficult to elucidate. Herein, we report the first detailed structural characterization of the (1→6)-β-linked side chains of Candida glabrata using high-field NMR. The (1→6)-β-linked side chains have an average length of 4 to 5 repeat units spaced every 21 repeat units along the (1→3)-linked polymer backbone. Computer modeling suggests that the side chains have a bent curve structure that allows for a flexible interconnection with parallel (1→3)-β-D-glucan polymers, and/or as a point of attachment for proteins. Based on these observations we propose new approaches to how (1→6)-β-linked side chains interconnect with neighboring glucan polymers in a manner that maximizes fungal cell wall strength, while also allowing for flexibility, or plasticity.https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0027614&type=printable |
| spellingShingle | Douglas W Lowman Lara J West Daniel W Bearden Michael F Wempe Trevor D Power Harry E Ensley Ken Haynes David L Williams Michael D Kruppa New insights into the structure of (1→3,1→6)-β-D-glucan side chains in the Candida glabrata cell wall. PLoS ONE |
| title | New insights into the structure of (1→3,1→6)-β-D-glucan side chains in the Candida glabrata cell wall. |
| title_full | New insights into the structure of (1→3,1→6)-β-D-glucan side chains in the Candida glabrata cell wall. |
| title_fullStr | New insights into the structure of (1→3,1→6)-β-D-glucan side chains in the Candida glabrata cell wall. |
| title_full_unstemmed | New insights into the structure of (1→3,1→6)-β-D-glucan side chains in the Candida glabrata cell wall. |
| title_short | New insights into the structure of (1→3,1→6)-β-D-glucan side chains in the Candida glabrata cell wall. |
| title_sort | new insights into the structure of 1 3 1 6 β d glucan side chains in the candida glabrata cell wall |
| url | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0027614&type=printable |
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