Population structure and genetic characteristics of Streptococcus pneumoniae isolates from children with chronic respiratory diseases

Objective. To study the population and genetic structure of S. pneumoniae isolated from children with cystic fibrosis (CF) and congenital malformations of the bronchi and lungs (CM) over the period 2011 to 2021, their serotype diversity in pre-vaccination and post-vaccination periods and susceptibi...

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Main Authors: Komyagina T.M., Tryapochkina A.S., Alyabieva N.M., Lazareva A.V., Gorinova Yu.V., Simonova O.I.
Format: Article
Language:Russian
Published: Interregional Association for Clinical Microbiology and Antimicrobial Chemotherapy 2024-12-01
Series:Клиническая микробиология и антимикробная химиотерапия
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Online Access:https://cmac-journal.ru/publication/2024/4/cmac-2024-t26-n4-p480/cmac-2024-t26-n4-p480.pdf
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author Komyagina T.M.
Tryapochkina A.S.
Alyabieva N.M.
Lazareva A.V.
Gorinova Yu.V.
Simonova O.I.
author_facet Komyagina T.M.
Tryapochkina A.S.
Alyabieva N.M.
Lazareva A.V.
Gorinova Yu.V.
Simonova O.I.
author_sort Komyagina T.M.
collection DOAJ
description Objective. To study the population and genetic structure of S. pneumoniae isolated from children with cystic fibrosis (CF) and congenital malformations of the bronchi and lungs (CM) over the period 2011 to 2021, their serotype diversity in pre-vaccination and post-vaccination periods and susceptibility to antimicrobials. Materials and Methods. A total of 140 S. pneumoniae isolates (during 2011-2021) from respiratory samples of patients with CM or CF were included in the study. For all isolates serotypes, antimicrobial susceptibility, resistance genes to β-lactams and macrolides, and sequence types were determined. Results. We observed a general increase in non-vaccine serotypes mainly due to serotype 11A. Antimicrobial resistance was higher in vaccine serotypes compared to non-vaccine serotypes. There was an increase in the number of S. pneumoniae isolates with reduced susceptibility to penicillin among vaccine serotypes. All ermB/mef-containing islated belonged to the cMLSB phenotype and demonstrated high levels of macrolide resistance and multidrug resistance (MDR). Conclusions. Continuous monitoring of the population structure of S. pneumoniae in children with chronic bronchopulmonary diseases is necessary. The detection of MDR serotypes with new sequence types indicates the great importance of continuing surveillance of antimicrobial resistance of S. pneumoniae.
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spelling doaj-art-11a85e9df1b842c8a370a2d08a11c3cd2025-08-20T01:54:57ZrusInterregional Association for Clinical Microbiology and Antimicrobial ChemotherapyКлиническая микробиология и антимикробная химиотерапия1684-43862686-95862024-12-0126448048610.36488/cmac.2024.4.480-486cmac-2024-t26-n4-p480Population structure and genetic characteristics of Streptococcus pneumoniae isolates from children with chronic respiratory diseasesKomyagina T.M.0Tryapochkina A.S.1Alyabieva N.M.2Lazareva A.V.3Gorinova Yu.V.4Simonova O.I.5National Medical Research Center for Children’s Health, Moscow, RussiaNational Medical Research Center for Children’s Health, Moscow, RussiaNational Medical Research Center for Children’s Health, Moscow, RussiaNational Medical Research Center for Children’s Health, Moscow, RussiaNational Medical Research Center for Children’s Health, Moscow, RussiaNational Medical Research Center for Children’s Health, Moscow, RussiaObjective. To study the population and genetic structure of S. pneumoniae isolated from children with cystic fibrosis (CF) and congenital malformations of the bronchi and lungs (CM) over the period 2011 to 2021, their serotype diversity in pre-vaccination and post-vaccination periods and susceptibility to antimicrobials. Materials and Methods. A total of 140 S. pneumoniae isolates (during 2011-2021) from respiratory samples of patients with CM or CF were included in the study. For all isolates serotypes, antimicrobial susceptibility, resistance genes to β-lactams and macrolides, and sequence types were determined. Results. We observed a general increase in non-vaccine serotypes mainly due to serotype 11A. Antimicrobial resistance was higher in vaccine serotypes compared to non-vaccine serotypes. There was an increase in the number of S. pneumoniae isolates with reduced susceptibility to penicillin among vaccine serotypes. All ermB/mef-containing islated belonged to the cMLSB phenotype and demonstrated high levels of macrolide resistance and multidrug resistance (MDR). Conclusions. Continuous monitoring of the population structure of S. pneumoniae in children with chronic bronchopulmonary diseases is necessary. The detection of MDR serotypes with new sequence types indicates the great importance of continuing surveillance of antimicrobial resistance of S. pneumoniae.https://cmac-journal.ru/publication/2024/4/cmac-2024-t26-n4-p480/cmac-2024-t26-n4-p480.pdfstreptococcus pneumoniaeepidemiologyserotypesantimicrobial resistancecystic fibrosiscongenital malformations of the bronchi and lungs
spellingShingle Komyagina T.M.
Tryapochkina A.S.
Alyabieva N.M.
Lazareva A.V.
Gorinova Yu.V.
Simonova O.I.
Population structure and genetic characteristics of Streptococcus pneumoniae isolates from children with chronic respiratory diseases
Клиническая микробиология и антимикробная химиотерапия
streptococcus pneumoniae
epidemiology
serotypes
antimicrobial resistance
cystic fibrosis
congenital malformations of the bronchi and lungs
title Population structure and genetic characteristics of Streptococcus pneumoniae isolates from children with chronic respiratory diseases
title_full Population structure and genetic characteristics of Streptococcus pneumoniae isolates from children with chronic respiratory diseases
title_fullStr Population structure and genetic characteristics of Streptococcus pneumoniae isolates from children with chronic respiratory diseases
title_full_unstemmed Population structure and genetic characteristics of Streptococcus pneumoniae isolates from children with chronic respiratory diseases
title_short Population structure and genetic characteristics of Streptococcus pneumoniae isolates from children with chronic respiratory diseases
title_sort population structure and genetic characteristics of streptococcus pneumoniae isolates from children with chronic respiratory diseases
topic streptococcus pneumoniae
epidemiology
serotypes
antimicrobial resistance
cystic fibrosis
congenital malformations of the bronchi and lungs
url https://cmac-journal.ru/publication/2024/4/cmac-2024-t26-n4-p480/cmac-2024-t26-n4-p480.pdf
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