Development of <i>Salvia officinalis</i>–Based Self-Emulsifying Systems for Dermal Application: Antioxidant, Anti-Inflammatory, and Skin Penetration Enhancement

Background/Objectives: The present study focused on the formulation and evaluation of novel topical systems containing <i>Salvia officinalis</i> (sage), emphasizing their antioxidant and anti-inflammatory properties. Sage, rich in carnosol, offers considerable therapeutic potential, yet...

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Main Authors: Krisztina Bodnár, Boglárka Papp, Dávid Sinka, Pálma Fehér, Zoltán Ujhelyi, István Lekli, Richárd Kajtár, Fruzsina Nacsa, Ildikó Bácskay, Liza Józsa
Format: Article
Language:English
Published: MDPI AG 2025-01-01
Series:Pharmaceutics
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Online Access:https://www.mdpi.com/1999-4923/17/2/140
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author Krisztina Bodnár
Boglárka Papp
Dávid Sinka
Pálma Fehér
Zoltán Ujhelyi
István Lekli
Richárd Kajtár
Fruzsina Nacsa
Ildikó Bácskay
Liza Józsa
author_facet Krisztina Bodnár
Boglárka Papp
Dávid Sinka
Pálma Fehér
Zoltán Ujhelyi
István Lekli
Richárd Kajtár
Fruzsina Nacsa
Ildikó Bácskay
Liza Józsa
author_sort Krisztina Bodnár
collection DOAJ
description Background/Objectives: The present study focused on the formulation and evaluation of novel topical systems containing <i>Salvia officinalis</i> (sage), emphasizing their antioxidant and anti-inflammatory properties. Sage, rich in carnosol, offers considerable therapeutic potential, yet its low water solubility limits its effectiveness in traditional formulations. The aim of our experimental work was to improve the solubility and thus bioavailability of the active ingredient by developing self-nano/microemulsifying drug delivery systems (SN/MEDDSs) with the help of Labrasol and Labrafil M as the nonionic surfactants, Transcutol HP as the co-surfactant, and isopropyl myristate as the oily phase. Methods: The formulations were characterized for droplet size, zeta potential, polydispersity index (PDI), encapsulation efficacy, and stability. The composition exhibiting the most favorable characteristics, with particle sizes falling within the nanoscale range, was incorporated into a cream and a gel, which were compared for their textural properties, carnosol penetration, biocompatibility and efficacy. Results: Release studies conducted using Franz diffusion cells demonstrated that the SNEDDS-based cream achieved up to 80% carnosol release, outperforming gels. The 2,2-diphenyl-1-picrylhydrazyl (DPPH) test and enzyme-linked immunosorbent assays (ELISA) showed strong efficacy, with an in vivo carrageenan-induced rat paw edema model revealing that the SNEDDS-based cream significantly reduced inflammation. Conclusions: These findings highlight the potential of SNEDDS-enhanced topical formulations in improving therapeutic outcomes. Further research is warranted to confirm their long-term safety and efficacy.
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spelling doaj-art-11a38377c4ed426f9bb3e0160da3f0c02025-08-20T02:03:42ZengMDPI AGPharmaceutics1999-49232025-01-0117214010.3390/pharmaceutics17020140Development of <i>Salvia officinalis</i>–Based Self-Emulsifying Systems for Dermal Application: Antioxidant, Anti-Inflammatory, and Skin Penetration EnhancementKrisztina Bodnár0Boglárka Papp1Dávid Sinka2Pálma Fehér3Zoltán Ujhelyi4István Lekli5Richárd Kajtár6Fruzsina Nacsa7Ildikó Bácskay8Liza Józsa9Department of Pharmaceutical Technology, Faculty of Pharmacy, University of Debrecen, 4032 Debrecen, HungaryDepartment of Pharmaceutical Technology, Faculty of Pharmacy, University of Debrecen, 4032 Debrecen, HungaryDepartment of Pharmaceutical Technology, Faculty of Pharmacy, University of Debrecen, 4032 Debrecen, HungaryDepartment of Pharmaceutical Technology, Faculty of Pharmacy, University of Debrecen, 4032 Debrecen, HungaryDepartment of Pharmaceutical Technology, Faculty of Pharmacy, University of Debrecen, 4032 Debrecen, HungaryDoctoral School of Pharmaceutical Sciences, University of Debrecen, 4032 Debrecen, HungaryDoctoral School of Pharmaceutical Sciences, University of Debrecen, 4032 Debrecen, HungaryMEDITOP Pharmaceutical Ltd., Pilisborosjeno Ady Endre Street 1, 2097 Pilisborosjeno, HungaryDepartment of Pharmaceutical Technology, Faculty of Pharmacy, University of Debrecen, 4032 Debrecen, HungaryDepartment of Pharmaceutical Technology, Faculty of Pharmacy, University of Debrecen, 4032 Debrecen, HungaryBackground/Objectives: The present study focused on the formulation and evaluation of novel topical systems containing <i>Salvia officinalis</i> (sage), emphasizing their antioxidant and anti-inflammatory properties. Sage, rich in carnosol, offers considerable therapeutic potential, yet its low water solubility limits its effectiveness in traditional formulations. The aim of our experimental work was to improve the solubility and thus bioavailability of the active ingredient by developing self-nano/microemulsifying drug delivery systems (SN/MEDDSs) with the help of Labrasol and Labrafil M as the nonionic surfactants, Transcutol HP as the co-surfactant, and isopropyl myristate as the oily phase. Methods: The formulations were characterized for droplet size, zeta potential, polydispersity index (PDI), encapsulation efficacy, and stability. The composition exhibiting the most favorable characteristics, with particle sizes falling within the nanoscale range, was incorporated into a cream and a gel, which were compared for their textural properties, carnosol penetration, biocompatibility and efficacy. Results: Release studies conducted using Franz diffusion cells demonstrated that the SNEDDS-based cream achieved up to 80% carnosol release, outperforming gels. The 2,2-diphenyl-1-picrylhydrazyl (DPPH) test and enzyme-linked immunosorbent assays (ELISA) showed strong efficacy, with an in vivo carrageenan-induced rat paw edema model revealing that the SNEDDS-based cream significantly reduced inflammation. Conclusions: These findings highlight the potential of SNEDDS-enhanced topical formulations in improving therapeutic outcomes. Further research is warranted to confirm their long-term safety and efficacy.https://www.mdpi.com/1999-4923/17/2/140<i>Salvia officinalis</i>dermal drug deliverySNEDDSanti-inflammatory effectantioxidant effect
spellingShingle Krisztina Bodnár
Boglárka Papp
Dávid Sinka
Pálma Fehér
Zoltán Ujhelyi
István Lekli
Richárd Kajtár
Fruzsina Nacsa
Ildikó Bácskay
Liza Józsa
Development of <i>Salvia officinalis</i>–Based Self-Emulsifying Systems for Dermal Application: Antioxidant, Anti-Inflammatory, and Skin Penetration Enhancement
Pharmaceutics
<i>Salvia officinalis</i>
dermal drug delivery
SNEDDS
anti-inflammatory effect
antioxidant effect
title Development of <i>Salvia officinalis</i>–Based Self-Emulsifying Systems for Dermal Application: Antioxidant, Anti-Inflammatory, and Skin Penetration Enhancement
title_full Development of <i>Salvia officinalis</i>–Based Self-Emulsifying Systems for Dermal Application: Antioxidant, Anti-Inflammatory, and Skin Penetration Enhancement
title_fullStr Development of <i>Salvia officinalis</i>–Based Self-Emulsifying Systems for Dermal Application: Antioxidant, Anti-Inflammatory, and Skin Penetration Enhancement
title_full_unstemmed Development of <i>Salvia officinalis</i>–Based Self-Emulsifying Systems for Dermal Application: Antioxidant, Anti-Inflammatory, and Skin Penetration Enhancement
title_short Development of <i>Salvia officinalis</i>–Based Self-Emulsifying Systems for Dermal Application: Antioxidant, Anti-Inflammatory, and Skin Penetration Enhancement
title_sort development of i salvia officinalis i based self emulsifying systems for dermal application antioxidant anti inflammatory and skin penetration enhancement
topic <i>Salvia officinalis</i>
dermal drug delivery
SNEDDS
anti-inflammatory effect
antioxidant effect
url https://www.mdpi.com/1999-4923/17/2/140
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