Identification, Characterization and Anti-inflammatory Activity of a New Flavonoid From Hibiscus mutabilis L.
Hibiscus mutabilis L (Malvaceae) was a traditional Chinese medicine with significant anti-inflammatory activity. We isolated 3 compounds from the flowers of H mutabilis L , including a new flavonoid, (1″R)-8-(1-(3,4-dihydroxyphenyl)ethyl)-3,3',5,7-tetrahydroxy-4′-methoxyl flavone ( 1 ), and 2 k...
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| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
SAGE Publishing
2024-10-01
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| Series: | Dose-Response |
| Online Access: | https://doi.org/10.1177/15593258241290130 |
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| Summary: | Hibiscus mutabilis L (Malvaceae) was a traditional Chinese medicine with significant anti-inflammatory activity. We isolated 3 compounds from the flowers of H mutabilis L , including a new flavonoid, (1″R)-8-(1-(3,4-dihydroxyphenyl)ethyl)-3,3',5,7-tetrahydroxy-4′-methoxyl flavone ( 1 ), and 2 known flavonoids ( 2-3 ). The structures of these compounds were elucidated by various spectroscopic methods. In addition, we evaluated the anti-inflammatory effect of compound 1 . The bioactivity assay showed that 1 significantly inhibit the excessive production of IL-6, TNF-α and NO in LPS-induced RAW264.7 macrophages. Western blot analysis demonstrated that 1 inhibit the expressions of COX-2 and iNOS protein in the macrophages induced by LPS in a concentration-dependent manner. In addition, 1 also significantly suppress the expression of the key proteins P65 and p-P65 in the NF- κ B signaling pathway. Our results revealed that 1 exert potential anti-inflammatory activity through inhibiting the activation of NF- κ B signaling pathway. |
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| ISSN: | 1559-3258 |