Chitosan treatment abrogates hypercholesterolemia-induced erythrocyte’s arginase activation
This study aimed to evaluate the protective effect of chitosan (CS) against hypercholesterolemia (HC) induced arginase activation and disruption of nitric oxide (NO) biosynthesis using erythrocytes as cellular model. Human erythrocytes were isolated and classified into eight groups. Next, cells were...
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| Format: | Article |
| Language: | English |
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Springer
2017-01-01
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| Series: | Saudi Pharmaceutical Journal |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1319016416300469 |
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| author | Gamaleldin I. Harisa Sabry M. Attia Khairy M.A. Zoheir Fars K. Alanazi |
| author_facet | Gamaleldin I. Harisa Sabry M. Attia Khairy M.A. Zoheir Fars K. Alanazi |
| author_sort | Gamaleldin I. Harisa |
| collection | DOAJ |
| description | This study aimed to evaluate the protective effect of chitosan (CS) against hypercholesterolemia (HC) induced arginase activation and disruption of nitric oxide (NO) biosynthesis using erythrocytes as cellular model. Human erythrocytes were isolated and classified into eight groups. Next, cells were treated with l-arginine (l-ARG), Nω-nitro-l-arginine methyl ester (l-NAME), CS or CS + l-ARG in the presence of normal plasma or cholesterol enriches plasma. Then, erythrocytes were incubated at 37 °C for 24 h. The present results revealed that, HC induced significant increase of cholesterol inclusion into erythrocytes membrane compared to control. Moreover, HC caused significant decrease in nitric oxide synthase (NOS) activity similar to l-NAME; however, arginase activity and arginase/NOS ratio significantly increased compared to control. On contrast, treatment of HC with, l-arginine, CS or CS plus l-arginine prevents HC induced cholesterol loading into erythrocytes membrane, NOS inhibition and arginase activation. This study suggested that CS could be protective agent against HC induced disruption of erythrocyte’s oxidative status and arginase activation. |
| format | Article |
| id | doaj-art-1199a59190194386b7f682fd0b145d8f |
| institution | Kabale University |
| issn | 1319-0164 |
| language | English |
| publishDate | 2017-01-01 |
| publisher | Springer |
| record_format | Article |
| series | Saudi Pharmaceutical Journal |
| spelling | doaj-art-1199a59190194386b7f682fd0b145d8f2025-08-20T03:54:12ZengSpringerSaudi Pharmaceutical Journal1319-01642017-01-0125112012710.1016/j.jsps.2016.05.007Chitosan treatment abrogates hypercholesterolemia-induced erythrocyte’s arginase activationGamaleldin I. Harisa0Sabry M. Attia1Khairy M.A. Zoheir2Fars K. Alanazi3Kayyali Chair for Pharmaceutical Industry, Department of Pharmaceutics, College of Pharmacy, King Saud University, Riyadh, Saudi ArabiaDepartments of Biochemistry, Pharmacology and Toxicology, College of Pharmacy, Al-Azhar University (Boys), Nasr City, Cairo, EgyptDepartment of Pharmacology and Toxicology, College of Pharmacy, King Saud University, Riyadh, Saudi ArabiaKayyali Chair for Pharmaceutical Industry, Department of Pharmaceutics, College of Pharmacy, King Saud University, Riyadh, Saudi ArabiaThis study aimed to evaluate the protective effect of chitosan (CS) against hypercholesterolemia (HC) induced arginase activation and disruption of nitric oxide (NO) biosynthesis using erythrocytes as cellular model. Human erythrocytes were isolated and classified into eight groups. Next, cells were treated with l-arginine (l-ARG), Nω-nitro-l-arginine methyl ester (l-NAME), CS or CS + l-ARG in the presence of normal plasma or cholesterol enriches plasma. Then, erythrocytes were incubated at 37 °C for 24 h. The present results revealed that, HC induced significant increase of cholesterol inclusion into erythrocytes membrane compared to control. Moreover, HC caused significant decrease in nitric oxide synthase (NOS) activity similar to l-NAME; however, arginase activity and arginase/NOS ratio significantly increased compared to control. On contrast, treatment of HC with, l-arginine, CS or CS plus l-arginine prevents HC induced cholesterol loading into erythrocytes membrane, NOS inhibition and arginase activation. This study suggested that CS could be protective agent against HC induced disruption of erythrocyte’s oxidative status and arginase activation.http://www.sciencedirect.com/science/article/pii/S1319016416300469ErythrocytesChitosanHypercholesterolemiaNitric oxideArginase |
| spellingShingle | Gamaleldin I. Harisa Sabry M. Attia Khairy M.A. Zoheir Fars K. Alanazi Chitosan treatment abrogates hypercholesterolemia-induced erythrocyte’s arginase activation Saudi Pharmaceutical Journal Erythrocytes Chitosan Hypercholesterolemia Nitric oxide Arginase |
| title | Chitosan treatment abrogates hypercholesterolemia-induced erythrocyte’s arginase activation |
| title_full | Chitosan treatment abrogates hypercholesterolemia-induced erythrocyte’s arginase activation |
| title_fullStr | Chitosan treatment abrogates hypercholesterolemia-induced erythrocyte’s arginase activation |
| title_full_unstemmed | Chitosan treatment abrogates hypercholesterolemia-induced erythrocyte’s arginase activation |
| title_short | Chitosan treatment abrogates hypercholesterolemia-induced erythrocyte’s arginase activation |
| title_sort | chitosan treatment abrogates hypercholesterolemia induced erythrocyte s arginase activation |
| topic | Erythrocytes Chitosan Hypercholesterolemia Nitric oxide Arginase |
| url | http://www.sciencedirect.com/science/article/pii/S1319016416300469 |
| work_keys_str_mv | AT gamaleldiniharisa chitosantreatmentabrogateshypercholesterolemiainducederythrocytesarginaseactivation AT sabrymattia chitosantreatmentabrogateshypercholesterolemiainducederythrocytesarginaseactivation AT khairymazoheir chitosantreatmentabrogateshypercholesterolemiainducederythrocytesarginaseactivation AT farskalanazi chitosantreatmentabrogateshypercholesterolemiainducederythrocytesarginaseactivation |