Regulation of Annexin I in Rheumatoid Synovial Cells by Glucocorticoids and Interleukin-1

<p>The glucocorticoid (GC)-induced antiinflammatory molecule annexin I is expressed in leukocytes and has antiinflammatory effects in animal models of arthritis, but the expression of annexin I in rheumatoid arthritis (RA) fibroblast-like synoviocytes (FLS) is unknown. We report the constituti...

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Format: Article
Language:English
Published: Wiley 2006-01-01
Series:Mediators of Inflammation
Online Access:http://www.hindawi.com/GetArticle.aspx?doi=10.1155/MI/2006/73835
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description <p>The glucocorticoid (GC)-induced antiinflammatory molecule annexin I is expressed in leukocytes and has antiinflammatory effects in animal models of arthritis, but the expression of annexin I in rheumatoid arthritis (RA) fibroblast-like synoviocytes (FLS) is unknown. We report the constitutive and dexamethasone (DEX)-inducible expression of annexin I in RA FLS. DEX increased FLS annexin I protein translocation and mRNA expression. Interleukin (IL)-1<mml:math alttext="$eta$"> <mml:mi>&#x03B2;</mml:mi> </mml:math> also induced annexin I translocation and mRNA but also increased intracellular protein. DEX and IL-1 had additive effects on annexin I mRNA, but DEX inhibited the inducing effect of IL-1<mml:math alttext="$eta$"> <mml:mi>&#x03B2;</mml:mi> </mml:math> on cell surface annexin I. These results indicate that glucocorticoids and IL-1<mml:math alttext="$eta$"> <mml:mi>&#x03B2;</mml:mi> </mml:math> upregulate the synthesis and translocation of annexin I in RA FLS, but interdependent signalling pathways are involved.</p>
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spelling doaj-art-11976d7448d541ad94663bbd52a8bb252025-08-20T02:01:54ZengWileyMediators of Inflammation0962-93512006-01-012006Regulation of Annexin I in Rheumatoid Synovial Cells by Glucocorticoids and Interleukin-1<p>The glucocorticoid (GC)-induced antiinflammatory molecule annexin I is expressed in leukocytes and has antiinflammatory effects in animal models of arthritis, but the expression of annexin I in rheumatoid arthritis (RA) fibroblast-like synoviocytes (FLS) is unknown. We report the constitutive and dexamethasone (DEX)-inducible expression of annexin I in RA FLS. DEX increased FLS annexin I protein translocation and mRNA expression. Interleukin (IL)-1<mml:math alttext="$eta$"> <mml:mi>&#x03B2;</mml:mi> </mml:math> also induced annexin I translocation and mRNA but also increased intracellular protein. DEX and IL-1 had additive effects on annexin I mRNA, but DEX inhibited the inducing effect of IL-1<mml:math alttext="$eta$"> <mml:mi>&#x03B2;</mml:mi> </mml:math> on cell surface annexin I. These results indicate that glucocorticoids and IL-1<mml:math alttext="$eta$"> <mml:mi>&#x03B2;</mml:mi> </mml:math> upregulate the synthesis and translocation of annexin I in RA FLS, but interdependent signalling pathways are involved.</p>http://www.hindawi.com/GetArticle.aspx?doi=10.1155/MI/2006/73835
spellingShingle Regulation of Annexin I in Rheumatoid Synovial Cells by Glucocorticoids and Interleukin-1
Mediators of Inflammation
title Regulation of Annexin I in Rheumatoid Synovial Cells by Glucocorticoids and Interleukin-1
title_full Regulation of Annexin I in Rheumatoid Synovial Cells by Glucocorticoids and Interleukin-1
title_fullStr Regulation of Annexin I in Rheumatoid Synovial Cells by Glucocorticoids and Interleukin-1
title_full_unstemmed Regulation of Annexin I in Rheumatoid Synovial Cells by Glucocorticoids and Interleukin-1
title_short Regulation of Annexin I in Rheumatoid Synovial Cells by Glucocorticoids and Interleukin-1
title_sort regulation of annexin i in rheumatoid synovial cells by glucocorticoids and interleukin 1
url http://www.hindawi.com/GetArticle.aspx?doi=10.1155/MI/2006/73835