Mapping genomic regions affecting sensitivity to bovine respiratory disease on chromosome X using selective DNA pooling
Abstract Bovine respiratory disease is a leading health problem in feedlot cattle. Identification of affecting genes is essential for selection for decrease sensitivity. Chromosome X is a special attractive target for gene mapping in light of reports on both sexual dimorphism in immunity and higher...
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Nature Portfolio
2025-02-01
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| Online Access: | https://doi.org/10.1038/s41598-025-89020-1 |
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| author | Ehud Lipkin Maria Giuseppina Strillacci Miri Cohen-Zinder Harel Eitam Moran Yishay Morris Soller Carlotta Ferrari Alessandro Bagnato Ariel Shabtay |
| author_facet | Ehud Lipkin Maria Giuseppina Strillacci Miri Cohen-Zinder Harel Eitam Moran Yishay Morris Soller Carlotta Ferrari Alessandro Bagnato Ariel Shabtay |
| author_sort | Ehud Lipkin |
| collection | DOAJ |
| description | Abstract Bovine respiratory disease is a leading health problem in feedlot cattle. Identification of affecting genes is essential for selection for decrease sensitivity. Chromosome X is a special attractive target for gene mapping in light of reports on both sexual dimorphism in immunity and higher susceptibility of males to this disease. However, diagnosis is challenging and clinical signs often go undetected. Kosher scoring of lung adhesions was used as a cost-effective proxy diagnosis. Selective DNA pooling was applied for cost-effective mapping of regions associated with sensitivity to the disease on chromosome X in Israeli Holstein male calves. A total of 9 regions were found, more than twice of any of the autosomes. All regions overlapped or were very close to previously reported regions. Bioinformatics survey found candidate-by-location genes in these regions. Functional analyses identified candidates-by-function among these genes. Network analyses connected the genes and found possible relations of the genes and the networks with morbidity, and specifically with sensitivity to bovine respiratory disease. The relatively large number of affecting regions and the candidate genes on the sex chromosome may explain part of the higher susceptibility of males and provide genomic and management targets for mitigating this disease. |
| format | Article |
| id | doaj-art-118ef04c49754867a935f715ff578956 |
| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Nature Portfolio |
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| series | Scientific Reports |
| spelling | doaj-art-118ef04c49754867a935f715ff5789562025-02-09T12:29:39ZengNature PortfolioScientific Reports2045-23222025-02-0115111210.1038/s41598-025-89020-1Mapping genomic regions affecting sensitivity to bovine respiratory disease on chromosome X using selective DNA poolingEhud Lipkin0Maria Giuseppina Strillacci1Miri Cohen-Zinder2Harel Eitam3Moran Yishay4Morris Soller5Carlotta Ferrari6Alessandro Bagnato7Ariel Shabtay8Department of Genetics, Hebrew University of JerusalemDepartment of Veterinary Medicine and Animal Sciences, Università degli Studi di MilanoSustainable Ruminants Production Lab, Newe-Ya’ar Research Center, Agricultural Research OrganizationSustainable Ruminants Production Lab, Newe-Ya’ar Research Center, Agricultural Research OrganizationSustainable Ruminants Production Lab, Newe-Ya’ar Research Center, Agricultural Research OrganizationDepartment of Genetics, Hebrew University of JerusalemDepartment of Veterinary Medicine and Animal Sciences, Università degli Studi di MilanoDepartment of Veterinary Medicine and Animal Sciences, Università degli Studi di MilanoSustainable Ruminants Production Lab, Newe-Ya’ar Research Center, Agricultural Research OrganizationAbstract Bovine respiratory disease is a leading health problem in feedlot cattle. Identification of affecting genes is essential for selection for decrease sensitivity. Chromosome X is a special attractive target for gene mapping in light of reports on both sexual dimorphism in immunity and higher susceptibility of males to this disease. However, diagnosis is challenging and clinical signs often go undetected. Kosher scoring of lung adhesions was used as a cost-effective proxy diagnosis. Selective DNA pooling was applied for cost-effective mapping of regions associated with sensitivity to the disease on chromosome X in Israeli Holstein male calves. A total of 9 regions were found, more than twice of any of the autosomes. All regions overlapped or were very close to previously reported regions. Bioinformatics survey found candidate-by-location genes in these regions. Functional analyses identified candidates-by-function among these genes. Network analyses connected the genes and found possible relations of the genes and the networks with morbidity, and specifically with sensitivity to bovine respiratory disease. The relatively large number of affecting regions and the candidate genes on the sex chromosome may explain part of the higher susceptibility of males and provide genomic and management targets for mitigating this disease.https://doi.org/10.1038/s41598-025-89020-1Bovine respiratory disease (BRD)Immunity sexual dimorphismKosher scoringQuantitative trait regions (QTLRs)Candidate genes |
| spellingShingle | Ehud Lipkin Maria Giuseppina Strillacci Miri Cohen-Zinder Harel Eitam Moran Yishay Morris Soller Carlotta Ferrari Alessandro Bagnato Ariel Shabtay Mapping genomic regions affecting sensitivity to bovine respiratory disease on chromosome X using selective DNA pooling Scientific Reports Bovine respiratory disease (BRD) Immunity sexual dimorphism Kosher scoring Quantitative trait regions (QTLRs) Candidate genes |
| title | Mapping genomic regions affecting sensitivity to bovine respiratory disease on chromosome X using selective DNA pooling |
| title_full | Mapping genomic regions affecting sensitivity to bovine respiratory disease on chromosome X using selective DNA pooling |
| title_fullStr | Mapping genomic regions affecting sensitivity to bovine respiratory disease on chromosome X using selective DNA pooling |
| title_full_unstemmed | Mapping genomic regions affecting sensitivity to bovine respiratory disease on chromosome X using selective DNA pooling |
| title_short | Mapping genomic regions affecting sensitivity to bovine respiratory disease on chromosome X using selective DNA pooling |
| title_sort | mapping genomic regions affecting sensitivity to bovine respiratory disease on chromosome x using selective dna pooling |
| topic | Bovine respiratory disease (BRD) Immunity sexual dimorphism Kosher scoring Quantitative trait regions (QTLRs) Candidate genes |
| url | https://doi.org/10.1038/s41598-025-89020-1 |
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