Glutamine Supplementation Attenuates Expressions of Adhesion Molecules and Chemokine Receptors on T Cells in a Murine Model of Acute Colitis
Background. Migration of T cells into the colon plays a major role in the pathogenesis in inflammatory bowel disease. This study investigated the effects of glutamine (Gln) supplementation on chemokine receptors and adhesion molecules expressed by T cells in mice with dextran sulfate sodium- (DSS-)...
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| Format: | Article |
| Language: | English |
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Wiley
2014-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/2014/837107 |
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| author | Yu-Chen Hou Jin-Ming Wu Ming-Yang Wang Ming-Hsun Wu Kuen-Yuan Chen Sung-Ling Yeh Ming-Tsan Lin |
| author_facet | Yu-Chen Hou Jin-Ming Wu Ming-Yang Wang Ming-Hsun Wu Kuen-Yuan Chen Sung-Ling Yeh Ming-Tsan Lin |
| author_sort | Yu-Chen Hou |
| collection | DOAJ |
| description | Background. Migration of T cells into the colon plays a major role in the pathogenesis in inflammatory bowel disease. This study investigated the effects of glutamine (Gln) supplementation on chemokine receptors and adhesion molecules expressed by T cells in mice with dextran sulfate sodium- (DSS-) induced colitis. Methods. C57BL/6 mice were fed either a standard diet or a Gln diet replacing 25% of the total nitrogen. After being fed the diets for 5 days, half of the mice from both groups were given 1.5% DSS in drinking water to induce colitis. Mice were killed after 5 days of DSS exposure. Results. DSS colitis resulted in higher expression levels of P-selectin glycoprotein ligand- (PSGL-) 1, leukocyte function-associated antigen- (LFA-) 1, and C-C chemokine receptor type 9 (CCR9) by T helper (Th) and cytotoxic T (Tc) cells, and mRNA levels of endothelial adhesion molecules in colons were upregulated. Gln supplementation decreased expressions of PSGL-1, LFA-1, and CCR9 by Th cells. Colonic gene expressions of endothelial adhesion molecules were also lower in Gln-colitis mice. Histological finding showed that colon infiltrating Th cells were less in the DSS group with Gln administration. Conclusions. Gln supplementation may ameliorate the inflammation of colitis possibly via suppression of T cell migration. |
| format | Article |
| id | doaj-art-118c3f46d36e4127b85eec48acebe37b |
| institution | OA Journals |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-118c3f46d36e4127b85eec48acebe37b2025-08-20T02:01:54ZengWileyMediators of Inflammation0962-93511466-18612014-01-01201410.1155/2014/837107837107Glutamine Supplementation Attenuates Expressions of Adhesion Molecules and Chemokine Receptors on T Cells in a Murine Model of Acute ColitisYu-Chen Hou0Jin-Ming Wu1Ming-Yang Wang2Ming-Hsun Wu3Kuen-Yuan Chen4Sung-Ling Yeh5Ming-Tsan Lin6Department of Surgery, National Taiwan University Hospital, Taipei 100, TaiwanDepartment of Surgery, National Taiwan University Hospital, Taipei 100, TaiwanDepartment of Surgery, National Taiwan University Hospital, Taipei 100, TaiwanDepartment of Surgery, National Taiwan University Hospital, Taipei 100, TaiwanDepartment of Surgery, National Taiwan University Hospital, Taipei 100, TaiwanSchool of Nutrition and Health Sciences, Taipei Medical University, Taipei 110, TaiwanDepartment of Surgery, National Taiwan University Hospital, Taipei 100, TaiwanBackground. Migration of T cells into the colon plays a major role in the pathogenesis in inflammatory bowel disease. This study investigated the effects of glutamine (Gln) supplementation on chemokine receptors and adhesion molecules expressed by T cells in mice with dextran sulfate sodium- (DSS-) induced colitis. Methods. C57BL/6 mice were fed either a standard diet or a Gln diet replacing 25% of the total nitrogen. After being fed the diets for 5 days, half of the mice from both groups were given 1.5% DSS in drinking water to induce colitis. Mice were killed after 5 days of DSS exposure. Results. DSS colitis resulted in higher expression levels of P-selectin glycoprotein ligand- (PSGL-) 1, leukocyte function-associated antigen- (LFA-) 1, and C-C chemokine receptor type 9 (CCR9) by T helper (Th) and cytotoxic T (Tc) cells, and mRNA levels of endothelial adhesion molecules in colons were upregulated. Gln supplementation decreased expressions of PSGL-1, LFA-1, and CCR9 by Th cells. Colonic gene expressions of endothelial adhesion molecules were also lower in Gln-colitis mice. Histological finding showed that colon infiltrating Th cells were less in the DSS group with Gln administration. Conclusions. Gln supplementation may ameliorate the inflammation of colitis possibly via suppression of T cell migration.http://dx.doi.org/10.1155/2014/837107 |
| spellingShingle | Yu-Chen Hou Jin-Ming Wu Ming-Yang Wang Ming-Hsun Wu Kuen-Yuan Chen Sung-Ling Yeh Ming-Tsan Lin Glutamine Supplementation Attenuates Expressions of Adhesion Molecules and Chemokine Receptors on T Cells in a Murine Model of Acute Colitis Mediators of Inflammation |
| title | Glutamine Supplementation Attenuates Expressions of Adhesion Molecules and Chemokine Receptors on T Cells in a Murine Model of Acute Colitis |
| title_full | Glutamine Supplementation Attenuates Expressions of Adhesion Molecules and Chemokine Receptors on T Cells in a Murine Model of Acute Colitis |
| title_fullStr | Glutamine Supplementation Attenuates Expressions of Adhesion Molecules and Chemokine Receptors on T Cells in a Murine Model of Acute Colitis |
| title_full_unstemmed | Glutamine Supplementation Attenuates Expressions of Adhesion Molecules and Chemokine Receptors on T Cells in a Murine Model of Acute Colitis |
| title_short | Glutamine Supplementation Attenuates Expressions of Adhesion Molecules and Chemokine Receptors on T Cells in a Murine Model of Acute Colitis |
| title_sort | glutamine supplementation attenuates expressions of adhesion molecules and chemokine receptors on t cells in a murine model of acute colitis |
| url | http://dx.doi.org/10.1155/2014/837107 |
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