Determination of Flavonoids, Phenolic Acids, and Xanthines in Mate Tea (Ilex paraguariensis St.-Hil.)
Raw material, different formulations of foods, and dietary supplements of mate demands control of the content of bioactive substances for which high performance thin layer chromatography (TLC), described here, presents simple and rapid approach for detections as well as quantification. Using TLC den...
Saved in:
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2013-01-01
|
| Series: | Journal of Analytical Methods in Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2013/658596 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832567985030561792 |
|---|---|
| author | Mirza Bojić Vicente Simon Haas Darija Šarić Željan Maleš |
| author_facet | Mirza Bojić Vicente Simon Haas Darija Šarić Željan Maleš |
| author_sort | Mirza Bojić |
| collection | DOAJ |
| description | Raw material, different formulations of foods, and dietary supplements of mate demands control of the content of bioactive substances for which high performance thin layer chromatography (TLC), described here, presents simple and rapid approach for detections as well as quantification. Using TLC densitometry, the following bioactive compounds were identified and quantified: chlorogenic acid (2.1 mg/g), caffeic acid (1.5 mg/g), rutin (5.2 mg/g), quercetin (2.2 mg/g), and kaempferol (4.5 mg/g). The results obtained with TLC densitometry for caffeine (5.4 mg/g) and theobromine (2.7 mg/g) show no statistical difference to the content of total xanthines (7.6 mg/g) obtained by UV-Vis spectrophotometry. Thus, TLC remains a technique of choice for simple and rapid analysis of great number of samples as well as a primary screening technique in plant analysis. |
| format | Article |
| id | doaj-art-11821fd213f14e25bb27132651f892cd |
| institution | Kabale University |
| issn | 2090-8865 2090-8873 |
| language | English |
| publishDate | 2013-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Analytical Methods in Chemistry |
| spelling | doaj-art-11821fd213f14e25bb27132651f892cd2025-02-03T00:59:57ZengWileyJournal of Analytical Methods in Chemistry2090-88652090-88732013-01-01201310.1155/2013/658596658596Determination of Flavonoids, Phenolic Acids, and Xanthines in Mate Tea (Ilex paraguariensis St.-Hil.)Mirza Bojić0Vicente Simon Haas1Darija Šarić2Željan Maleš3Faculty of Pharmacy and Biochemistry, University of Zagreb, A. Kovačića 1, 10000 Zagreb, CroatiaUniversidade Regional Integrada, URI-Farma, Rua Universidade das Missões 464, 98802-470 Santo Ângelo, RS, BrazilFaculty of Pharmacy and Biochemistry, University of Zagreb, A. Kovačića 1, 10000 Zagreb, CroatiaFaculty of Pharmacy and Biochemistry, University of Zagreb, A. Kovačića 1, 10000 Zagreb, CroatiaRaw material, different formulations of foods, and dietary supplements of mate demands control of the content of bioactive substances for which high performance thin layer chromatography (TLC), described here, presents simple and rapid approach for detections as well as quantification. Using TLC densitometry, the following bioactive compounds were identified and quantified: chlorogenic acid (2.1 mg/g), caffeic acid (1.5 mg/g), rutin (5.2 mg/g), quercetin (2.2 mg/g), and kaempferol (4.5 mg/g). The results obtained with TLC densitometry for caffeine (5.4 mg/g) and theobromine (2.7 mg/g) show no statistical difference to the content of total xanthines (7.6 mg/g) obtained by UV-Vis spectrophotometry. Thus, TLC remains a technique of choice for simple and rapid analysis of great number of samples as well as a primary screening technique in plant analysis.http://dx.doi.org/10.1155/2013/658596 |
| spellingShingle | Mirza Bojić Vicente Simon Haas Darija Šarić Željan Maleš Determination of Flavonoids, Phenolic Acids, and Xanthines in Mate Tea (Ilex paraguariensis St.-Hil.) Journal of Analytical Methods in Chemistry |
| title | Determination of Flavonoids, Phenolic Acids, and Xanthines in Mate Tea (Ilex paraguariensis St.-Hil.) |
| title_full | Determination of Flavonoids, Phenolic Acids, and Xanthines in Mate Tea (Ilex paraguariensis St.-Hil.) |
| title_fullStr | Determination of Flavonoids, Phenolic Acids, and Xanthines in Mate Tea (Ilex paraguariensis St.-Hil.) |
| title_full_unstemmed | Determination of Flavonoids, Phenolic Acids, and Xanthines in Mate Tea (Ilex paraguariensis St.-Hil.) |
| title_short | Determination of Flavonoids, Phenolic Acids, and Xanthines in Mate Tea (Ilex paraguariensis St.-Hil.) |
| title_sort | determination of flavonoids phenolic acids and xanthines in mate tea ilex paraguariensis st hil |
| url | http://dx.doi.org/10.1155/2013/658596 |
| work_keys_str_mv | AT mirzabojic determinationofflavonoidsphenolicacidsandxanthinesinmateteailexparaguariensissthil AT vicentesimonhaas determinationofflavonoidsphenolicacidsandxanthinesinmateteailexparaguariensissthil AT darijasaric determinationofflavonoidsphenolicacidsandxanthinesinmateteailexparaguariensissthil AT zeljanmales determinationofflavonoidsphenolicacidsandxanthinesinmateteailexparaguariensissthil |