IL-27 effects on HIVGag-specific CD4 and CD8 T cell function
BackgroundIn people with HIV (PWH) and suppressed viral replication by antiretroviral therapy persistent T cell activation and inflammation are important contributors of the increased risk of morbidity and mortality. CD8 T cells express checkpoint receptors and are dysfunctional. IL-27, a member of...
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Frontiers Media S.A.
2025-06-01
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| Series: | Frontiers in Virology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fviro.2025.1600802/full |
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| author | Maryam Abdussamad Grace Katz Jie Cheng Chinmayee Mehta Fernando Andres-Martin Danial Mahmood Princy Kumar Marta Catalfamo |
| author_facet | Maryam Abdussamad Grace Katz Jie Cheng Chinmayee Mehta Fernando Andres-Martin Danial Mahmood Princy Kumar Marta Catalfamo |
| author_sort | Maryam Abdussamad |
| collection | DOAJ |
| description | BackgroundIn people with HIV (PWH) and suppressed viral replication by antiretroviral therapy persistent T cell activation and inflammation are important contributors of the increased risk of morbidity and mortality. CD8 T cells express checkpoint receptors and are dysfunctional. IL-27, a member of the IL-6/IL-12 family has shown anti-viral properties against various human viruses, including HIV. The role of IL-27 on HIV-specific T cells remains unclear. We hypothesized that IL-27 will enhance the function of HIV-specific T cells.MethodsIL-27 effects on T cell function was evaluated by measuring cytokine secretion, proliferation, and cytotoxicity. ResultsOur findings show that IL-27 upregulates cytokine secretion and cytotoxic potential, and trafficking of proliferating HIV-specific CD8 T cells expressing checkpoint receptors TIGIT and PD-1. Unbiased clustering analysis showed that IL-27 may have differential effects on distinct populations of HIV-specific T cells. ConclusionAltogether these results suggest that IL-27 may enhance T cell function in the setting of chronic HIV infection. |
| format | Article |
| id | doaj-art-117868d9bfdd40ab8cc5d685f88eb489 |
| institution | DOAJ |
| issn | 2673-818X |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Virology |
| spelling | doaj-art-117868d9bfdd40ab8cc5d685f88eb4892025-08-20T03:20:27ZengFrontiers Media S.A.Frontiers in Virology2673-818X2025-06-01510.3389/fviro.2025.16008021600802IL-27 effects on HIVGag-specific CD4 and CD8 T cell functionMaryam Abdussamad0Grace Katz1Jie Cheng2Chinmayee Mehta3Fernando Andres-Martin4Danial Mahmood5Princy Kumar6Marta Catalfamo7Department of Microbiology and Immunology, Georgetown University School of Medicine, Washington, DC, United StatesDepartment of Microbiology and Immunology, Georgetown University School of Medicine, Washington, DC, United StatesDepartment of Microbiology and Immunology, Georgetown University School of Medicine, Washington, DC, United StatesDepartment of Microbiology and Immunology, Georgetown University School of Medicine, Washington, DC, United StatesDepartment of Microbiology and Immunology, Georgetown University School of Medicine, Washington, DC, United StatesDivision of Infectious Diseases and Travel Medicine, Georgetown University School of Medicine, Washington, DC, United StatesDivision of Infectious Diseases and Travel Medicine, Georgetown University School of Medicine, Washington, DC, United StatesDepartment of Microbiology and Immunology, Georgetown University School of Medicine, Washington, DC, United StatesBackgroundIn people with HIV (PWH) and suppressed viral replication by antiretroviral therapy persistent T cell activation and inflammation are important contributors of the increased risk of morbidity and mortality. CD8 T cells express checkpoint receptors and are dysfunctional. IL-27, a member of the IL-6/IL-12 family has shown anti-viral properties against various human viruses, including HIV. The role of IL-27 on HIV-specific T cells remains unclear. We hypothesized that IL-27 will enhance the function of HIV-specific T cells.MethodsIL-27 effects on T cell function was evaluated by measuring cytokine secretion, proliferation, and cytotoxicity. ResultsOur findings show that IL-27 upregulates cytokine secretion and cytotoxic potential, and trafficking of proliferating HIV-specific CD8 T cells expressing checkpoint receptors TIGIT and PD-1. Unbiased clustering analysis showed that IL-27 may have differential effects on distinct populations of HIV-specific T cells. ConclusionAltogether these results suggest that IL-27 may enhance T cell function in the setting of chronic HIV infection.https://www.frontiersin.org/articles/10.3389/fviro.2025.1600802/fullIL27HIVT cell immune activationHIV-specific T cellsIL-27 effects on T cells |
| spellingShingle | Maryam Abdussamad Grace Katz Jie Cheng Chinmayee Mehta Fernando Andres-Martin Danial Mahmood Princy Kumar Marta Catalfamo IL-27 effects on HIVGag-specific CD4 and CD8 T cell function Frontiers in Virology IL27 HIV T cell immune activation HIV-specific T cells IL-27 effects on T cells |
| title | IL-27 effects on HIVGag-specific CD4 and CD8 T cell function |
| title_full | IL-27 effects on HIVGag-specific CD4 and CD8 T cell function |
| title_fullStr | IL-27 effects on HIVGag-specific CD4 and CD8 T cell function |
| title_full_unstemmed | IL-27 effects on HIVGag-specific CD4 and CD8 T cell function |
| title_short | IL-27 effects on HIVGag-specific CD4 and CD8 T cell function |
| title_sort | il 27 effects on hivgag specific cd4 and cd8 t cell function |
| topic | IL27 HIV T cell immune activation HIV-specific T cells IL-27 effects on T cells |
| url | https://www.frontiersin.org/articles/10.3389/fviro.2025.1600802/full |
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