Commensal microorganisms ameliorate the adverse effects of high wheat starch diet on the growth performance, glucose and lipid metabolisms in juvenile largemouth bass, Micropterus salmoides
Largemouth bass (Micropterus salmoides) is poor at utilizing starch, and it is not clear whether commensal microorganisms can alleviate the adverse influences of high wheat starch in M. salmoides. Here, twelve autochthonous microorganisms with amylase producing abilities were isolated from the M. sa...
Saved in:
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2024-12-01
|
| Series: | Aquaculture Reports |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2352513424005404 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850057131223416832 |
|---|---|
| author | Bi-Yun Zhang Hong-Ling Yang Ze-Hui Yuan Xiao-Ou Sun Jia-Hao Yin Jun-Ji Xu Guo-He Cai Yun-Zhang Sun |
| author_facet | Bi-Yun Zhang Hong-Ling Yang Ze-Hui Yuan Xiao-Ou Sun Jia-Hao Yin Jun-Ji Xu Guo-He Cai Yun-Zhang Sun |
| author_sort | Bi-Yun Zhang |
| collection | DOAJ |
| description | Largemouth bass (Micropterus salmoides) is poor at utilizing starch, and it is not clear whether commensal microorganisms can alleviate the adverse influences of high wheat starch in M. salmoides. Here, twelve autochthonous microorganisms with amylase producing abilities were isolated from the M. salmoides fed with diets containing 6 %, 12 %, or 18 % starch. Among them, Citrobacter freundii F1, Bacillus altitudinis F7, Enterococcus casseliflavus D12, Plesiomonas shigelloides D15, Bacillus cabrialesii D16, Citrobacter portucalensis K25 and Serratia oryzae K28 showed no hemolytic activity and were safety for M. salmoides. A 42-d feeding experiment was performed to study the functions of the seven microorganisms. A total of 810 fish (5.9 ± 0.01 g) were equally distributed into 9 groups. The control groups C6 and C18 were respectively fed with 6 % and 18 % wheat starch diets, the groups F1, F7, D12, D15, D16, K25 and K28 were fed with 18 % wheat starch diets respectively supplemented with 1.0×108 CFU/g C. freundii F1, B. altitudinis F7, E. casseliflavus D12, P. shigelloides D15, B. cabrialesii D16, C. portucalensis K25 and S. oryzae K28. The results indicated that compared with 6 % starch, 18 % level inhibited the growth performance, increased the hepatic glycogen and fat contents, improved the activities of glycolysis-related enzymes (hexokinase (HK), glucokinase (GK), phosphofructokinase (PFK), pyruvate kinase (PK)), decreased the activities of gluconeogenesis-related enzymes, pyruvate carboxylase (PC) and phosphoenolpyruvate carboxykinase (PEPCK), promoted the activity of lipid synthases-related enzyme carnitine palmitoyl transferaseⅠ (CPT1) and reduced the activity of lipolytic enzymes-related lipoprotein lipase (LPL). C. freundii F1, P. shigelloides D15, C. portucalensis K25 and S. oryzae K28 had relatively poor alleviating effects, while B. altitudinis F7, E. casseliflavus D12 and B. cabrialesii D16 significantly promoted the final body weight (FBW), weight gain rate (WGR), specific growth rate (SGR), protein efficiency ratio (PER), decreased feed conversion rate (FCR), significantly reduced the hepatic glycogen and fat contents, decreased the activities of glycolysis-related enzymes (HK, GK, PK), improved the activities of gluconeogenesis-related enzymes (PC, PEPCK), reduced the activity of lipid synthases-related enzyme CPT1 (P < 0.05), which proved that these three strains could effectively alleviate the negative effects of high starch in M. salmoides. In conclusion, among the seven autochthonous microorganisms, B. altitudinis F7, E. casseliflavus D12 and B. cabrialesii D16 presented better potential advantages in improving glucose and lipid metabolisms, thus promoting the growth performance of M. salmoides fed with high wheat starch diet. |
| format | Article |
| id | doaj-art-116fde7423cf4c1db93ebe4e9290296c |
| institution | DOAJ |
| issn | 2352-5134 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Aquaculture Reports |
| spelling | doaj-art-116fde7423cf4c1db93ebe4e9290296c2025-08-20T02:51:31ZengElsevierAquaculture Reports2352-51342024-12-013910245210.1016/j.aqrep.2024.102452Commensal microorganisms ameliorate the adverse effects of high wheat starch diet on the growth performance, glucose and lipid metabolisms in juvenile largemouth bass, Micropterus salmoidesBi-Yun Zhang0Hong-Ling Yang1Ze-Hui Yuan2Xiao-Ou Sun3Jia-Hao Yin4Jun-Ji Xu5Guo-He Cai6Yun-Zhang Sun7State Key Laboratory of Mariculture Breeding, Fisheries College of Jimei University, Xiamen 361021, China; Xiamen Key Laboratory for Feed Quality Testing and Safety Evaluation, Fisheries College, Jimei University, Xiamen 361021, ChinaState Key Laboratory of Mariculture Breeding, Fisheries College of Jimei University, Xiamen 361021, China; Xiamen Key Laboratory for Feed Quality Testing and Safety Evaluation, Fisheries College, Jimei University, Xiamen 361021, China; The Key Laboratory of Healthy Mariculture for the East China Sea, Ministry of Agriculture, Fisheries College, Jimei University, Xiamen 361021, ChinaState Key Laboratory of Mariculture Breeding, Fisheries College of Jimei University, Xiamen 361021, China; Xiamen Key Laboratory for Feed Quality Testing and Safety Evaluation, Fisheries College, Jimei University, Xiamen 361021, ChinaState Key Laboratory of Mariculture Breeding, Fisheries College of Jimei University, Xiamen 361021, ChinaState Key Laboratory of Mariculture Breeding, Fisheries College of Jimei University, Xiamen 361021, ChinaState Key Laboratory of Mariculture Breeding, Fisheries College of Jimei University, Xiamen 361021, ChinaState Key Laboratory of Mariculture Breeding, Fisheries College of Jimei University, Xiamen 361021, China; Xiamen Key Laboratory for Feed Quality Testing and Safety Evaluation, Fisheries College, Jimei University, Xiamen 361021, China; The Key Laboratory of Healthy Mariculture for the East China Sea, Ministry of Agriculture, Fisheries College, Jimei University, Xiamen 361021, China; Correspondence to: Fisheries College, Jimei University, Xiamen, Fujian 361021, China.State Key Laboratory of Mariculture Breeding, Fisheries College of Jimei University, Xiamen 361021, China; Xiamen Key Laboratory for Feed Quality Testing and Safety Evaluation, Fisheries College, Jimei University, Xiamen 361021, China; The Key Laboratory of Healthy Mariculture for the East China Sea, Ministry of Agriculture, Fisheries College, Jimei University, Xiamen 361021, China; Engineering Research Center of the Modern Technology for Eel Industry, Ministry of Education, Xiamen 361021, China; Correspondence to: Fisheries College, Jimei University, Xiamen, Fujian 361021, China.Largemouth bass (Micropterus salmoides) is poor at utilizing starch, and it is not clear whether commensal microorganisms can alleviate the adverse influences of high wheat starch in M. salmoides. Here, twelve autochthonous microorganisms with amylase producing abilities were isolated from the M. salmoides fed with diets containing 6 %, 12 %, or 18 % starch. Among them, Citrobacter freundii F1, Bacillus altitudinis F7, Enterococcus casseliflavus D12, Plesiomonas shigelloides D15, Bacillus cabrialesii D16, Citrobacter portucalensis K25 and Serratia oryzae K28 showed no hemolytic activity and were safety for M. salmoides. A 42-d feeding experiment was performed to study the functions of the seven microorganisms. A total of 810 fish (5.9 ± 0.01 g) were equally distributed into 9 groups. The control groups C6 and C18 were respectively fed with 6 % and 18 % wheat starch diets, the groups F1, F7, D12, D15, D16, K25 and K28 were fed with 18 % wheat starch diets respectively supplemented with 1.0×108 CFU/g C. freundii F1, B. altitudinis F7, E. casseliflavus D12, P. shigelloides D15, B. cabrialesii D16, C. portucalensis K25 and S. oryzae K28. The results indicated that compared with 6 % starch, 18 % level inhibited the growth performance, increased the hepatic glycogen and fat contents, improved the activities of glycolysis-related enzymes (hexokinase (HK), glucokinase (GK), phosphofructokinase (PFK), pyruvate kinase (PK)), decreased the activities of gluconeogenesis-related enzymes, pyruvate carboxylase (PC) and phosphoenolpyruvate carboxykinase (PEPCK), promoted the activity of lipid synthases-related enzyme carnitine palmitoyl transferaseⅠ (CPT1) and reduced the activity of lipolytic enzymes-related lipoprotein lipase (LPL). C. freundii F1, P. shigelloides D15, C. portucalensis K25 and S. oryzae K28 had relatively poor alleviating effects, while B. altitudinis F7, E. casseliflavus D12 and B. cabrialesii D16 significantly promoted the final body weight (FBW), weight gain rate (WGR), specific growth rate (SGR), protein efficiency ratio (PER), decreased feed conversion rate (FCR), significantly reduced the hepatic glycogen and fat contents, decreased the activities of glycolysis-related enzymes (HK, GK, PK), improved the activities of gluconeogenesis-related enzymes (PC, PEPCK), reduced the activity of lipid synthases-related enzyme CPT1 (P < 0.05), which proved that these three strains could effectively alleviate the negative effects of high starch in M. salmoides. In conclusion, among the seven autochthonous microorganisms, B. altitudinis F7, E. casseliflavus D12 and B. cabrialesii D16 presented better potential advantages in improving glucose and lipid metabolisms, thus promoting the growth performance of M. salmoides fed with high wheat starch diet.http://www.sciencedirect.com/science/article/pii/S2352513424005404Micropterus salmoidesHigh starch dietAutochthonous probioticsGlucose metabolismLipid metabolism |
| spellingShingle | Bi-Yun Zhang Hong-Ling Yang Ze-Hui Yuan Xiao-Ou Sun Jia-Hao Yin Jun-Ji Xu Guo-He Cai Yun-Zhang Sun Commensal microorganisms ameliorate the adverse effects of high wheat starch diet on the growth performance, glucose and lipid metabolisms in juvenile largemouth bass, Micropterus salmoides Aquaculture Reports Micropterus salmoides High starch diet Autochthonous probiotics Glucose metabolism Lipid metabolism |
| title | Commensal microorganisms ameliorate the adverse effects of high wheat starch diet on the growth performance, glucose and lipid metabolisms in juvenile largemouth bass, Micropterus salmoides |
| title_full | Commensal microorganisms ameliorate the adverse effects of high wheat starch diet on the growth performance, glucose and lipid metabolisms in juvenile largemouth bass, Micropterus salmoides |
| title_fullStr | Commensal microorganisms ameliorate the adverse effects of high wheat starch diet on the growth performance, glucose and lipid metabolisms in juvenile largemouth bass, Micropterus salmoides |
| title_full_unstemmed | Commensal microorganisms ameliorate the adverse effects of high wheat starch diet on the growth performance, glucose and lipid metabolisms in juvenile largemouth bass, Micropterus salmoides |
| title_short | Commensal microorganisms ameliorate the adverse effects of high wheat starch diet on the growth performance, glucose and lipid metabolisms in juvenile largemouth bass, Micropterus salmoides |
| title_sort | commensal microorganisms ameliorate the adverse effects of high wheat starch diet on the growth performance glucose and lipid metabolisms in juvenile largemouth bass micropterus salmoides |
| topic | Micropterus salmoides High starch diet Autochthonous probiotics Glucose metabolism Lipid metabolism |
| url | http://www.sciencedirect.com/science/article/pii/S2352513424005404 |
| work_keys_str_mv | AT biyunzhang commensalmicroorganismsamelioratetheadverseeffectsofhighwheatstarchdietonthegrowthperformanceglucoseandlipidmetabolismsinjuvenilelargemouthbassmicropterussalmoides AT honglingyang commensalmicroorganismsamelioratetheadverseeffectsofhighwheatstarchdietonthegrowthperformanceglucoseandlipidmetabolismsinjuvenilelargemouthbassmicropterussalmoides AT zehuiyuan commensalmicroorganismsamelioratetheadverseeffectsofhighwheatstarchdietonthegrowthperformanceglucoseandlipidmetabolismsinjuvenilelargemouthbassmicropterussalmoides AT xiaoousun commensalmicroorganismsamelioratetheadverseeffectsofhighwheatstarchdietonthegrowthperformanceglucoseandlipidmetabolismsinjuvenilelargemouthbassmicropterussalmoides AT jiahaoyin commensalmicroorganismsamelioratetheadverseeffectsofhighwheatstarchdietonthegrowthperformanceglucoseandlipidmetabolismsinjuvenilelargemouthbassmicropterussalmoides AT junjixu commensalmicroorganismsamelioratetheadverseeffectsofhighwheatstarchdietonthegrowthperformanceglucoseandlipidmetabolismsinjuvenilelargemouthbassmicropterussalmoides AT guohecai commensalmicroorganismsamelioratetheadverseeffectsofhighwheatstarchdietonthegrowthperformanceglucoseandlipidmetabolismsinjuvenilelargemouthbassmicropterussalmoides AT yunzhangsun commensalmicroorganismsamelioratetheadverseeffectsofhighwheatstarchdietonthegrowthperformanceglucoseandlipidmetabolismsinjuvenilelargemouthbassmicropterussalmoides |