In Vitro Antioxidant and Cancer Inhibitory Activity of a Colored Avocado Seed Extract
Avocado (Persea americana) seeds have been used traditionally for a number of health-related indications. Because of its high polyphenol content, we investigated the potential antioxidant and anticancer effects of a colored avocado seed extract (CASE). CASE exhibited an oxygen radical acceptance cap...
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| Format: | Article |
| Language: | English |
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Wiley
2019-01-01
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| Series: | International Journal of Food Science |
| Online Access: | http://dx.doi.org/10.1155/2019/6509421 |
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| author | Deepti Dabas Ryan J. Elias Gregory R. Ziegler Joshua D. Lambert |
| author_facet | Deepti Dabas Ryan J. Elias Gregory R. Ziegler Joshua D. Lambert |
| author_sort | Deepti Dabas |
| collection | DOAJ |
| description | Avocado (Persea americana) seeds have been used traditionally for a number of health-related indications. Because of its high polyphenol content, we investigated the potential antioxidant and anticancer effects of a colored avocado seed extract (CASE). CASE exhibited an oxygen radical acceptance capacity value of 2012 ± 300 trolox equivalents/mg. CASE reduced lipid hydroperoxide formation in an oil-in-water emulsion (33% reduction at 500 μg/mL). CASE dose-dependently reduced the viability of human breast (MCF7), lung (H1299), colon (HT29), and prostate (LNCaP) cancer cells in vitro. The half maximal inhibitory concentrations ranged from 19 to 132 μg/mL after treatment for 48 h. CASE treatment downregulated the expression of cyclin D1 and E2 in LNCaP cells. This was associated with cell G0/G1 phase cycle arrest. CASE also dose-dependently induced apoptosis in LNCaP cells. CASE reduced nuclear translocation of nuclear factor κB, a prosurvival signal. Further studies are needed to examine these effects in in vivo models. |
| format | Article |
| id | doaj-art-116535b9d1ed44eaa61e361ae0a72939 |
| institution | Kabale University |
| issn | 2356-7015 2314-5765 |
| language | English |
| publishDate | 2019-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Food Science |
| spelling | doaj-art-116535b9d1ed44eaa61e361ae0a729392025-08-20T03:54:11ZengWileyInternational Journal of Food Science2356-70152314-57652019-01-01201910.1155/2019/65094216509421In Vitro Antioxidant and Cancer Inhibitory Activity of a Colored Avocado Seed ExtractDeepti Dabas0Ryan J. Elias1Gregory R. Ziegler2Joshua D. Lambert3Department of Food Science, The Pennsylvania State University, University Park, PA 16802, USADepartment of Food Science, The Pennsylvania State University, University Park, PA 16802, USADepartment of Food Science, The Pennsylvania State University, University Park, PA 16802, USADepartment of Food Science, The Pennsylvania State University, University Park, PA 16802, USAAvocado (Persea americana) seeds have been used traditionally for a number of health-related indications. Because of its high polyphenol content, we investigated the potential antioxidant and anticancer effects of a colored avocado seed extract (CASE). CASE exhibited an oxygen radical acceptance capacity value of 2012 ± 300 trolox equivalents/mg. CASE reduced lipid hydroperoxide formation in an oil-in-water emulsion (33% reduction at 500 μg/mL). CASE dose-dependently reduced the viability of human breast (MCF7), lung (H1299), colon (HT29), and prostate (LNCaP) cancer cells in vitro. The half maximal inhibitory concentrations ranged from 19 to 132 μg/mL after treatment for 48 h. CASE treatment downregulated the expression of cyclin D1 and E2 in LNCaP cells. This was associated with cell G0/G1 phase cycle arrest. CASE also dose-dependently induced apoptosis in LNCaP cells. CASE reduced nuclear translocation of nuclear factor κB, a prosurvival signal. Further studies are needed to examine these effects in in vivo models.http://dx.doi.org/10.1155/2019/6509421 |
| spellingShingle | Deepti Dabas Ryan J. Elias Gregory R. Ziegler Joshua D. Lambert In Vitro Antioxidant and Cancer Inhibitory Activity of a Colored Avocado Seed Extract International Journal of Food Science |
| title | In Vitro Antioxidant and Cancer Inhibitory Activity of a Colored Avocado Seed Extract |
| title_full | In Vitro Antioxidant and Cancer Inhibitory Activity of a Colored Avocado Seed Extract |
| title_fullStr | In Vitro Antioxidant and Cancer Inhibitory Activity of a Colored Avocado Seed Extract |
| title_full_unstemmed | In Vitro Antioxidant and Cancer Inhibitory Activity of a Colored Avocado Seed Extract |
| title_short | In Vitro Antioxidant and Cancer Inhibitory Activity of a Colored Avocado Seed Extract |
| title_sort | in vitro antioxidant and cancer inhibitory activity of a colored avocado seed extract |
| url | http://dx.doi.org/10.1155/2019/6509421 |
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