In Vitro Antioxidant and Cancer Inhibitory Activity of a Colored Avocado Seed Extract

Avocado (Persea americana) seeds have been used traditionally for a number of health-related indications. Because of its high polyphenol content, we investigated the potential antioxidant and anticancer effects of a colored avocado seed extract (CASE). CASE exhibited an oxygen radical acceptance cap...

Full description

Saved in:
Bibliographic Details
Main Authors: Deepti Dabas, Ryan J. Elias, Gregory R. Ziegler, Joshua D. Lambert
Format: Article
Language:English
Published: Wiley 2019-01-01
Series:International Journal of Food Science
Online Access:http://dx.doi.org/10.1155/2019/6509421
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Avocado (Persea americana) seeds have been used traditionally for a number of health-related indications. Because of its high polyphenol content, we investigated the potential antioxidant and anticancer effects of a colored avocado seed extract (CASE). CASE exhibited an oxygen radical acceptance capacity value of 2012 ± 300 trolox equivalents/mg. CASE reduced lipid hydroperoxide formation in an oil-in-water emulsion (33% reduction at 500 μg/mL). CASE dose-dependently reduced the viability of human breast (MCF7), lung (H1299), colon (HT29), and prostate (LNCaP) cancer cells in vitro. The half maximal inhibitory concentrations ranged from 19 to 132 μg/mL after treatment for 48 h. CASE treatment downregulated the expression of cyclin D1 and E2 in LNCaP cells. This was associated with cell G0/G1 phase cycle arrest. CASE also dose-dependently induced apoptosis in LNCaP cells. CASE reduced nuclear translocation of nuclear factor κB, a prosurvival signal. Further studies are needed to examine these effects in in vivo models.
ISSN:2356-7015
2314-5765