Constitutive interaction between neutrophils and von Willebrand factor in peripheral blood
Abstract Neutrophils are known to be able to recruit von Willebrand factor (VWF) through Neutrophil Extracellular Traps (NETs) during inflammation. However, the interaction between neutrophils with plasma VWF at physiological condition remains poorly understood. In this study, we demonstrate that pe...
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Nature Portfolio
2025-07-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-025-10321-6 |
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| author | Yan Wan Min Gao Jueyi Zhu Qingqing Mu Yue Pan Yifan Chen Ting Yang Wei Deng |
| author_facet | Yan Wan Min Gao Jueyi Zhu Qingqing Mu Yue Pan Yifan Chen Ting Yang Wei Deng |
| author_sort | Yan Wan |
| collection | DOAJ |
| description | Abstract Neutrophils are known to be able to recruit von Willebrand factor (VWF) through Neutrophil Extracellular Traps (NETs) during inflammation. However, the interaction between neutrophils with plasma VWF at physiological condition remains poorly understood. In this study, we demonstrate that peripheral neutrophils constitutively carry surface-bound VWF in a NETs-independent manner. Depletion of peripheral neutrophils in mice elevated plasma VWF levels without affecting its synthesis or secretion. A similar interaction in human blood was also observed as demonstrated from the analysis of 23 healthy human donors. Human VWF binding to neutrophils is independent of gender and age; however, individuals with non-O blood types exhibit significantly lower VWF levels on neutrophils compared to those with type O blood. Furthermore, we found that the isolated human neutrophils bind stronger to the neuraminidase-treated VWF than untreated VWF. PSGL-1 and β2-integrins, two neutrophil membrane receptors, seemingly mediate this interaction since antibodies targeting both receptors showed a significant attenuating role here. These findings suggest a constitutive interaction between neutrophils and VWF in peripheral blood, in which the desialylation VWF seems play a major role here. Both PSGL-1 and β2-integrins receptors on neutrophil contribute to this interaction. Our study reveals a function of neutrophils beyond their established antimicrobial roles. |
| format | Article |
| id | doaj-art-115be835767f4e75bac1af9f015c2d5c |
| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | Nature Portfolio |
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| spelling | doaj-art-115be835767f4e75bac1af9f015c2d5c2025-08-20T03:46:00ZengNature PortfolioScientific Reports2045-23222025-07-0115111210.1038/s41598-025-10321-6Constitutive interaction between neutrophils and von Willebrand factor in peripheral bloodYan Wan0Min Gao1Jueyi Zhu2Qingqing Mu3Yue Pan4Yifan Chen5Ting Yang6Wei Deng7Cyrus Tang Medical Institute and Collaborative Innovation Center of Hematology, State Key Laboratory of Radiation Medicine and Protection, Soochow UniversityCyrus Tang Medical Institute and Collaborative Innovation Center of Hematology, State Key Laboratory of Radiation Medicine and Protection, Soochow UniversityCyrus Tang Medical Institute and Collaborative Innovation Center of Hematology, State Key Laboratory of Radiation Medicine and Protection, Soochow UniversityCyrus Tang Medical Institute and Collaborative Innovation Center of Hematology, State Key Laboratory of Radiation Medicine and Protection, Soochow UniversityCyrus Tang Medical Institute and Collaborative Innovation Center of Hematology, State Key Laboratory of Radiation Medicine and Protection, Soochow UniversityFujian Medical UniversityThe Second Department of Hematology, Binhai Campus of the First Affiliated Hospital, National Regional Medical Center, Fujian Medical UniversityCyrus Tang Medical Institute and Collaborative Innovation Center of Hematology, State Key Laboratory of Radiation Medicine and Protection, Soochow UniversityAbstract Neutrophils are known to be able to recruit von Willebrand factor (VWF) through Neutrophil Extracellular Traps (NETs) during inflammation. However, the interaction between neutrophils with plasma VWF at physiological condition remains poorly understood. In this study, we demonstrate that peripheral neutrophils constitutively carry surface-bound VWF in a NETs-independent manner. Depletion of peripheral neutrophils in mice elevated plasma VWF levels without affecting its synthesis or secretion. A similar interaction in human blood was also observed as demonstrated from the analysis of 23 healthy human donors. Human VWF binding to neutrophils is independent of gender and age; however, individuals with non-O blood types exhibit significantly lower VWF levels on neutrophils compared to those with type O blood. Furthermore, we found that the isolated human neutrophils bind stronger to the neuraminidase-treated VWF than untreated VWF. PSGL-1 and β2-integrins, two neutrophil membrane receptors, seemingly mediate this interaction since antibodies targeting both receptors showed a significant attenuating role here. These findings suggest a constitutive interaction between neutrophils and VWF in peripheral blood, in which the desialylation VWF seems play a major role here. Both PSGL-1 and β2-integrins receptors on neutrophil contribute to this interaction. Our study reveals a function of neutrophils beyond their established antimicrobial roles.https://doi.org/10.1038/s41598-025-10321-6 |
| spellingShingle | Yan Wan Min Gao Jueyi Zhu Qingqing Mu Yue Pan Yifan Chen Ting Yang Wei Deng Constitutive interaction between neutrophils and von Willebrand factor in peripheral blood Scientific Reports |
| title | Constitutive interaction between neutrophils and von Willebrand factor in peripheral blood |
| title_full | Constitutive interaction between neutrophils and von Willebrand factor in peripheral blood |
| title_fullStr | Constitutive interaction between neutrophils and von Willebrand factor in peripheral blood |
| title_full_unstemmed | Constitutive interaction between neutrophils and von Willebrand factor in peripheral blood |
| title_short | Constitutive interaction between neutrophils and von Willebrand factor in peripheral blood |
| title_sort | constitutive interaction between neutrophils and von willebrand factor in peripheral blood |
| url | https://doi.org/10.1038/s41598-025-10321-6 |
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