Embryonic macrophages orchestrate niche cell homeostasis for the establishment of the definitive hematopoietic stem cell pool

Abstract Embryonic macrophages emerge before the onset of definitive hematopoiesis, seed into discrete tissues and contribute to specialized resident macrophages throughout life. Presence of embryonic macrophages in the bone marrow and functional impact on hematopoietic stem cells (HSC) or the niche...

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Main Authors: Gülce Perçin, Konstantin Riege, Julia Fröbel, Jonas Metz, Stephan Culemann, Mathias Lesche, Susanne Reinhardt, Thomas Höfer, Steve Hoffmann, Claudia Waskow
Format: Article
Language:English
Published: Nature Portfolio 2025-05-01
Series:Nature Communications
Online Access:https://doi.org/10.1038/s41467-025-59059-9
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author Gülce Perçin
Konstantin Riege
Julia Fröbel
Jonas Metz
Stephan Culemann
Mathias Lesche
Susanne Reinhardt
Thomas Höfer
Steve Hoffmann
Claudia Waskow
author_facet Gülce Perçin
Konstantin Riege
Julia Fröbel
Jonas Metz
Stephan Culemann
Mathias Lesche
Susanne Reinhardt
Thomas Höfer
Steve Hoffmann
Claudia Waskow
author_sort Gülce Perçin
collection DOAJ
description Abstract Embryonic macrophages emerge before the onset of definitive hematopoiesis, seed into discrete tissues and contribute to specialized resident macrophages throughout life. Presence of embryonic macrophages in the bone marrow and functional impact on hematopoietic stem cells (HSC) or the niche remains unclear. Here we show that bone marrow macrophages consist of two ontogenetically distinct cell populations from embryonic and adult origin. Newborn mice lacking embryonic macrophages have decreased HSC numbers in the bone marrow suggesting an important function for embryo-derived macrophages in orchestrating HSC trafficking around birth. The establishment of a normal cellular niche space in the bone marrow critically depends on embryonic macrophages that are important for the development of mesenchymal stromal cells, but not other non-hematopoietic niche cells, providing evidence for a specific role for embryo-derived macrophages in the establishment of the niche environment pivotal for the establishment of a normally sized HSC pool.
format Article
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institution Kabale University
issn 2041-1723
language English
publishDate 2025-05-01
publisher Nature Portfolio
record_format Article
series Nature Communications
spelling doaj-art-11353fa0d2e84b8b99c4c4aabe8337ed2025-08-20T03:53:58ZengNature PortfolioNature Communications2041-17232025-05-0116111410.1038/s41467-025-59059-9Embryonic macrophages orchestrate niche cell homeostasis for the establishment of the definitive hematopoietic stem cell poolGülce Perçin0Konstantin Riege1Julia Fröbel2Jonas Metz3Stephan Culemann4Mathias Lesche5Susanne Reinhardt6Thomas Höfer7Steve Hoffmann8Claudia Waskow9Immunology of Aging, Leibniz Institute on Aging - Fritz Lipmann Institute (FLI)Computational Biology of Aging, Leibniz Institute on Aging - Fritz Lipmann Institute (FLI)Immunology of Aging, Leibniz Institute on Aging - Fritz Lipmann Institute (FLI)Theoretical Systems Biology, German Cancer Research CenterImmunology of Aging, Leibniz Institute on Aging - Fritz Lipmann Institute (FLI)DRESDEN-concept Genome Center, c/o CMCB Center for Molecular and Cellular Bioengineering Technology Platform of the TUD Dresden University of TechnologyDRESDEN-concept Genome Center, c/o CMCB Center for Molecular and Cellular Bioengineering Technology Platform of the TUD Dresden University of TechnologyTheoretical Systems Biology, German Cancer Research CenterComputational Biology of Aging, Leibniz Institute on Aging - Fritz Lipmann Institute (FLI)Immunology of Aging, Leibniz Institute on Aging - Fritz Lipmann Institute (FLI)Abstract Embryonic macrophages emerge before the onset of definitive hematopoiesis, seed into discrete tissues and contribute to specialized resident macrophages throughout life. Presence of embryonic macrophages in the bone marrow and functional impact on hematopoietic stem cells (HSC) or the niche remains unclear. Here we show that bone marrow macrophages consist of two ontogenetically distinct cell populations from embryonic and adult origin. Newborn mice lacking embryonic macrophages have decreased HSC numbers in the bone marrow suggesting an important function for embryo-derived macrophages in orchestrating HSC trafficking around birth. The establishment of a normal cellular niche space in the bone marrow critically depends on embryonic macrophages that are important for the development of mesenchymal stromal cells, but not other non-hematopoietic niche cells, providing evidence for a specific role for embryo-derived macrophages in the establishment of the niche environment pivotal for the establishment of a normally sized HSC pool.https://doi.org/10.1038/s41467-025-59059-9
spellingShingle Gülce Perçin
Konstantin Riege
Julia Fröbel
Jonas Metz
Stephan Culemann
Mathias Lesche
Susanne Reinhardt
Thomas Höfer
Steve Hoffmann
Claudia Waskow
Embryonic macrophages orchestrate niche cell homeostasis for the establishment of the definitive hematopoietic stem cell pool
Nature Communications
title Embryonic macrophages orchestrate niche cell homeostasis for the establishment of the definitive hematopoietic stem cell pool
title_full Embryonic macrophages orchestrate niche cell homeostasis for the establishment of the definitive hematopoietic stem cell pool
title_fullStr Embryonic macrophages orchestrate niche cell homeostasis for the establishment of the definitive hematopoietic stem cell pool
title_full_unstemmed Embryonic macrophages orchestrate niche cell homeostasis for the establishment of the definitive hematopoietic stem cell pool
title_short Embryonic macrophages orchestrate niche cell homeostasis for the establishment of the definitive hematopoietic stem cell pool
title_sort embryonic macrophages orchestrate niche cell homeostasis for the establishment of the definitive hematopoietic stem cell pool
url https://doi.org/10.1038/s41467-025-59059-9
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