Full-Length Transcriptome Sequencing and hsp Gene Family Analysis Provide New Insights into the Stress Response Mechanisms of <i>Mystus guttatus</i>
<i>Mystus guttatus</i>, a second-class protected species in China, has undergone severe population decline due to anthropogenic and environmental pressures, yet conservation efforts are hindered by limited genomic resources and a lack of mechanistic insights into its stress response syst...
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| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2025-07-01
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| Series: | Biology |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2079-7737/14/7/840 |
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| Summary: | <i>Mystus guttatus</i>, a second-class protected species in China, has undergone severe population decline due to anthropogenic and environmental pressures, yet conservation efforts are hindered by limited genomic resources and a lack of mechanistic insights into its stress response systems. Here, the first full-length transcriptome of <i>M. guttatus</i> was generated via SMRT sequencing. A total of 32,647 full-length transcripts were obtained, with an average length of 1783 bp. After structure and function annotation of full-length transcripts, 30,977 genes, 1670 transcription factors (TF), 918 alternative splicing (AS), and 11,830 simple sequence repeats (SSR) were identified. In order to further explore the stress resistance of <i>M. guttatus</i>, 93 genes belonging to the heat shock protein (HSP) family were identified and categorized into HSP70 and HSP90 subgroups. After phylogenetic analysis and selective stress analysis, it was discovered that the <i>hsp</i> family has suffered purifying selection and gene loss, potentially contributing to a decrease in the stress resilience and population of <i>M. guttatus.</i> Using protein interaction network and molecular docking tools, we observed the intricate interplay among HSPs and discovered HSP70-HOP-HSP90 interaction, which is an essential stress response mechanism. Our study sequenced the first full-length transcriptome of <i>M. guttatus</i> to enhance its genomic resources for its conservation and breeding and provide new insights into the future study of stress response mechanisms on <i>M. guttatus</i>. |
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| ISSN: | 2079-7737 |