Production of High Viscosity Chitosan from Biologically Purified Chitin Isolated by Microbial Fermentation and Deproteinization
The objective of this study was to produce high viscosity chitosan from shrimp chitin prepared by using a two-step biological treatment process: decalcification and deproteinization. Glucose was fermented with Lactobacillus pentosus L7 to lactic acid. At a pH of 3.9±0.1, the calcium carbonate of th...
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| Format: | Article |
| Language: | English |
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Wiley
2014-01-01
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| Series: | International Journal of Polymer Science |
| Online Access: | http://dx.doi.org/10.1155/2014/162173 |
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| author | Ekkalak Ploydee Saipin Chaiyanan |
| author_facet | Ekkalak Ploydee Saipin Chaiyanan |
| author_sort | Ekkalak Ploydee |
| collection | DOAJ |
| description | The objective of this study was to produce high viscosity chitosan from shrimp chitin prepared by using a two-step biological treatment process: decalcification and deproteinization. Glucose was fermented with Lactobacillus pentosus L7 to lactic acid. At a pH of 3.9±0.1, the calcium carbonate of the shells was solubilized in 48 hours. The amounts of residual calcium in the form of ash (1.4±0.5%) and crude protein (23.2±2.5%) were further eliminated by the activity of proteolytic Bacillus thuringiensis SA. After decalcification and deproteinization of the shrimp shells, residual calcium and crude protein of shrimp chitin flakes were 1.7±0.4% and 3.8±1.3%, respectively. Chitin was deacetylated with 50% NaOH at 121°C for 5 hours. After deacetylation, the chitosan had residual calcium, crude protein content, and degree of acetylation of 1.6±0.6%, 0.4±0.3%, and 83.2±1.5%, respectively. The viscosity of chitosan prepared from chitin extracted by this two-step biological process was 1,007±14.7 mPa·s, whereas chitosan prepared from chemically processed chitin had a viscosity of 323±15.6 mPa·s, indicating that biologically purified chitin gave chitosan with a high quality. |
| format | Article |
| id | doaj-art-111f55d94e924724965fde42f090ebca |
| institution | OA Journals |
| issn | 1687-9422 1687-9430 |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Polymer Science |
| spelling | doaj-art-111f55d94e924724965fde42f090ebca2025-08-20T02:02:19ZengWileyInternational Journal of Polymer Science1687-94221687-94302014-01-01201410.1155/2014/162173162173Production of High Viscosity Chitosan from Biologically Purified Chitin Isolated by Microbial Fermentation and DeproteinizationEkkalak Ploydee0Saipin Chaiyanan1Department of Microbiology, Faculty of Science, King Mongkut’s University of Technology Thonburi, Pracha Uthit Road, Bangkok 10140, ThailandDepartment of Microbiology, Faculty of Science, King Mongkut’s University of Technology Thonburi, Pracha Uthit Road, Bangkok 10140, ThailandThe objective of this study was to produce high viscosity chitosan from shrimp chitin prepared by using a two-step biological treatment process: decalcification and deproteinization. Glucose was fermented with Lactobacillus pentosus L7 to lactic acid. At a pH of 3.9±0.1, the calcium carbonate of the shells was solubilized in 48 hours. The amounts of residual calcium in the form of ash (1.4±0.5%) and crude protein (23.2±2.5%) were further eliminated by the activity of proteolytic Bacillus thuringiensis SA. After decalcification and deproteinization of the shrimp shells, residual calcium and crude protein of shrimp chitin flakes were 1.7±0.4% and 3.8±1.3%, respectively. Chitin was deacetylated with 50% NaOH at 121°C for 5 hours. After deacetylation, the chitosan had residual calcium, crude protein content, and degree of acetylation of 1.6±0.6%, 0.4±0.3%, and 83.2±1.5%, respectively. The viscosity of chitosan prepared from chitin extracted by this two-step biological process was 1,007±14.7 mPa·s, whereas chitosan prepared from chemically processed chitin had a viscosity of 323±15.6 mPa·s, indicating that biologically purified chitin gave chitosan with a high quality.http://dx.doi.org/10.1155/2014/162173 |
| spellingShingle | Ekkalak Ploydee Saipin Chaiyanan Production of High Viscosity Chitosan from Biologically Purified Chitin Isolated by Microbial Fermentation and Deproteinization International Journal of Polymer Science |
| title | Production of High Viscosity Chitosan from Biologically Purified Chitin Isolated by Microbial Fermentation and Deproteinization |
| title_full | Production of High Viscosity Chitosan from Biologically Purified Chitin Isolated by Microbial Fermentation and Deproteinization |
| title_fullStr | Production of High Viscosity Chitosan from Biologically Purified Chitin Isolated by Microbial Fermentation and Deproteinization |
| title_full_unstemmed | Production of High Viscosity Chitosan from Biologically Purified Chitin Isolated by Microbial Fermentation and Deproteinization |
| title_short | Production of High Viscosity Chitosan from Biologically Purified Chitin Isolated by Microbial Fermentation and Deproteinization |
| title_sort | production of high viscosity chitosan from biologically purified chitin isolated by microbial fermentation and deproteinization |
| url | http://dx.doi.org/10.1155/2014/162173 |
| work_keys_str_mv | AT ekkalakploydee productionofhighviscositychitosanfrombiologicallypurifiedchitinisolatedbymicrobialfermentationanddeproteinization AT saipinchaiyanan productionofhighviscositychitosanfrombiologicallypurifiedchitinisolatedbymicrobialfermentationanddeproteinization |